1、May 2009DEUTSCHE NORM English price group 14No part of this standard may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 13.060.70!$W_1“1526014www.
2、din.deDDIN EN ISO 11348-3Water quality Determination of the inhibitory effect of water samples on the lightPart 3: Method using freeze-dried bacteria (ISO 11348-3:2007)English version of DIN EN ISO 11348-3:2009-05Wasserbeschaffenheit Bestimmung der Hemmwirkung von Wasserproben auf die Lichtemission
3、vonTeil 3: Verfahren mit gefriergetrockneten Bakterien (ISO 11348-3:2007)Englische Fassung DIN EN ISO 11348-3:2009-05SupersedesDIN EN ISO 11348-3:1999-04www.beuth.deemission of Vibrio fischeri (Luminescent bacteria test) Vibrio fischeri (Leuchtbakterientest) Document comprises 31 pagesDIN EN ISO 113
4、48-3:2009-05 This standard is part of the series Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung Testverfahren mit Wasserorganismen (Gruppe L) (German standard methods for the examination of water, waste water and sludge Test methods using water organisms (group L) and desc
5、ribes method L 53. National foreword This standard has been prepared by Technical Committee ISO/TC 147 “Water quality” in collaboration with Technical Committee CEN/TC 230 “Water analysis” (Secretariat: DIN, Germany). The responsible German body involved in its preparation was the Normenausschuss Wa
6、sserwesen (Water Practice Standards Committee), Technical Committees NA 119-01-03-05-01 AK Biotests and NA 119-01-03 AA Wasseruntersuchung. This standard has been published to implement the Water Framework Directive (WFD), Directive 2000/60/EC of the European Parliament and of the Council of 23 Octo
7、ber 2000 establishing a framework for Community action in the field of water policy1). ISO 11348 consists of the following parts, under the general title Water quality Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test): Part 1
8、: Method using freshly prepared bacteria Part 2: Method using liquid-dried bacteria Part 3: Method using freeze-dried bacteria Designation of method L 53 “Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) Part 3: Method using
9、 freeze-dried bacteria”: Method DIN EN ISO 11348-3 L 53 Expert assistance and specialized laboratories will be required to perform the analysis described in this standard. Existing safety regulations are to be taken into account. Depending on the objective of the analysis, a check shall be made on a
10、 case-by-case basis as to whether and to what extent additional conditions will have to be specified. Standard methods published as DIN Standards are obtainable from Beuth Verlag GmbH, either individually or grouped in volumes. The standard methods included in the loose-leaf publication entitled Deu
11、tsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung will continue to be published by Wiley-VCH Verlag and Beuth Verlag GmbH. 1)Registered in the DITR database of DIN Software GmbH, available from: Beuth Verlag GmbH, 10772 Berlin. 2 DIN EN ISO 11348-3:2009-05 All standard methods r
12、elevant to the Abwasserverordnung (Waste Water Regulation) (AbwV) included in the new Regulation on Section 7a of the Gesetz zur Ordnung des Wasserhaushaltes (German Water Management Act) concerning Anforderungen an das Einleiten von Abwasser in Gewsser together with the Abwasserverordnung and the G
13、esetz zur Ordnung des Wasserhaushalts have been published by Beuth Verlag GmbH as a loose-leaf collection Analysenverfahren in der Abwasserverordnung Rechtsvorschriften und Normen (Supplements). Standard methods or draft standards bearing the group title “German standard methods for the examination
14、of water, waste water and sludge” are classified under the following categories (main titles): General information (group A) (DIN 38402) Sensory analysis (group B) (DIN 38403) Physical and physicochemical parameters (group C) (DIN 38404) Anions (group D) (DIN 38405) Cations (group E) (DIN 38406) Sub
15、stance group analysis (group F) (DIN 38407) Gaseous constituents (group G) (DIN 38408) Parameters characterizing effects and substances (group H) (DIN 38409) Biological-ecological methods of analysis (group M) (DIN 38410) Microbiological methods (group K) (DIN 38411) Test methods using water organis
16、ms (group L) (DIN 38412) Individual constituents (group P) (DIN 38413) Sludge and sediments (group S) (DIN 38414) Bio-assays with microorganisms (group T) (DIN 38415) In addition to the methods described in the DIN 38402 to DIN 38415 series of standards, there are a number of European and Internatio
17、nal Standards available as DIN EN, DIN EN ISO and DIN ISO Standards, which also form part of the collection of German standard methods. Information on Parts of these series of standards that have already been published can be obtained from the offices of the Normenausschuss Wasserwesen, telephone +4
18、9 30 2601-2448, or from Beuth Verlag GmbH, Burggrafenstr. 6, 10787 Berlin. The DIN Standards corresponding to the International Standards referred to in the EN are as follows: ISO 5667-16 DIN EN ISO 5667-16 ISO 5814 DIN EN 25814 ISO 10253 DIN EN ISO 10253 3 DIN EN ISO 11348-3:2009-05 Amendments This
19、 standard differs from DIN EN ISO 11348-3:1999-04 as follows: a) The scope has been extended to include: sea water; sediment eluates (fresh water, brackish and sea water); single substances, diluted in water. b) Clause 4 “Interferences” has been revised: the oxygen concentration of readily biodegrad
20、able nutrients is no longer specified; reference is now made to Bibliography 1; specifications regarding interferences due to samples with extreme pH values have been included; when testing salt-water samples, possible inferences as a result of stimulation effects of bioluminescence are to be taken
21、into account. c) Clause 5 “Reagents and materials”: the concentration of the 3,5-Dichlorophenol reference substance has been changed and the purity has been specified; commercially available reference substance solutions may now be used. d) Modifications relating to the preparation of the procedure:
22、 apparatus: the permissible temperature deviation within one test has been changed from 0,2 C to 0,3 C; sampling: samples may now be stored at 18 C for periods of up to two months (instead of two weeks); sample preparation: the oxygen concentration is to be measured in accordance with ISO 5814 and s
23、amples having an oxygen concentration of less than 3 mg/l are to be oxygenated by aeration or stirring; e) Modifications relating to the procedure: two parallel determinations have been specified for the tests; the permissible temperature of the stock suspension has been changed from 3 C 3 C to 4 C
24、3 C. f) A further method for the calculation of the concentration-effect relationship has been included (e.g. as in ISO/TS 20281). g) In Annex B, the volumes of the test and control batches have been modified and a dilution scheme has been included. h) Annex D “Testing salt water samples with the lu
25、minescent bacteria test with freshly cultured bacteria” has been added. i) The standard has been editorially revised. Previous editions DIN 38412-34: 1991-03, 1997-07 DIN EN ISO 11348-3: 1999-04 4 DIN EN ISO 11348-3:2009-05 National Annex NA (informative) Bibliography DIN EN 25814, Water quality Det
26、ermination of dissolved oxygen by the electrochemical probe method DIN EN ISO 5667-16, Water quality Sampling Part 16: Guidance on biotesting of samples DIN EN ISO 10253, Water quality Marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricornutum 5 DIN EN ISO 11348-3:20
27、09-05 6 This page is intentionally blank EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 11348-3 November 2008 ICS 13.060.70 Supersedes EN ISO 11348-3:1998 English Version Water quality Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Lumines
28、cent bacteria test) Part 3: Method using freeze-dried bacteria (ISO 11348-3:2007) Qualit de leau Dtermination de leffet inhibiteur dchantillons deau sur la luminescence de Vibrio fischeri (Essai de bactries luminescentes) Partie 3: Mthode utilisant des bactries lyophilises (ISO 11348-3:2007) Wasserb
29、eschaffenheit Bestimmung der Hemmwirkung von Wasserproben auf die Lichtemission von Vibrio fischeri (Leuchtbakterientest) Teil 3: Verfahren mit gefriergetrockneten Bakterien (ISO 11348-3:2007) This European Standard was approved by CEN on 29 October 2008. CEN members are bound to comply with the CEN
30、/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to
31、 any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as theofficial versions
32、. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain,
33、 Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: rue de Stassart, 36 B-1050 Brussels 2008 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Me
34、mbers. Ref. No. EN ISO 11348-3:2008: EContents Page Foreword 2 Introduction 4 1 Scope . 5 2 Normative references . 5 3 Principle. 6 4 Interferences . 6 5 Reagents and materials . 6 6 Apparatus 77 Sampling and sample pretreatment 8 8 Procedure 8 9 Evaluation 10 10 Expression of results . 12 11 Criter
35、ia of validity 14 12 Precision 14 13 Test report . 14 Annex A (informative) Colour-correction method. 15 Annex B (informative) Dilution level D Preparation of the dilution series . 18 Annex C (informative) Precision data. 21 Annex D (informative) Testing salt water samples with the luminescent bacte
36、ria test with freeze-dried bacteria. 22 Bibliography . 25 DIN EN ISO 11348-3:2009-05 EN ISO 11348-3:2008 (E) 2DIN EN ISO 11348-3:2009-05 EN ISO 11348-3:2008 (E) 3Foreword The text of ISO 11348-3:2007 has been prepared by Technical Committee ISO/TC 147 “Water quality” of the International Organizatio
37、n for Standardization (ISO) and has been taken over as EN ISO 11348-3:2008 by Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement
38、, at the latest by May 2009, and conflicting national standards shall be withdrawn at the latest by May 2009. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or
39、all such patent rights. This document supersedes EN ISO 11348-3:1998. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia
40、, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. Endorsement notice The text of ISO 11348-3:2007 has been approved by CEN
41、as a EN ISO 11348-3:2008 without any modification. Introduction The measurements specified in ISO 11348 can be carried out using freshly prepared bacteria, as well as freeze-dried or liquid-dried bacterial preparations. Standardized work carried out by DIN Normenausschuss Wasserwesen and ISO/TC 147/
42、SC 5/WG 1 has shown that, in special cases, these different techniques may give different results, especially in the presence of heavy metals. Such varying sensitivity is caused by differences in media composition used in the preparation of freeze-dried or liquid-dried bacteria. These protective med
43、ia influence the bioavailability of toxicants and/or the light emission of luminescent bacteria. This means that the origin and type of preparation need to be taken into account when interpreting the results. This may be difficult sometimes, as freeze-dried and liquid-dried bacteria may be obtained
44、from different suppliers. This, in turn, can mean that the composition is not known in detail and therefore cannot be interpreted by the user. For this reason, in addition to toxicity measurements with liquid-dried bacteria (ISO 11348-2) and freshly prepared bacteria (ISO 11348-1), a procedure with
45、freeze-dried bacteria is described in this part of ISO 11348, the performance of which can be interpreted by the user in every detail. The laboratories responsible for the results have the opportunity to select the most suitable technique based on expert judgement and information about the water sam
46、ple to be tested. DIN EN ISO 11348-3:2009-05 EN ISO 11348-3:2008 (E) 4WARNING Persons using this part of ISO 11348 should be familiar with normal laboratory practice. This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of th
47、e user to establish appropriate safety and health practices and to ensure compliance with any national regulatory conditions. IMPORTANT It is absolutely essential that tests conducted in accordance with this part of ISO 11348 be carried out by suitably trained staff. 1 Scope ISO 11348 describes thre
48、e methods for determining the inhibition of the luminescence emitted by the marine bacterium Vibrio fischeri (NRRL B-11177). This part of ISO 11348 specifies a method using freeze-dried bacteria. This method is applicable to: waste water; aqueous extracts and leachates; fresh water (surface and grou
49、nd water); sea and brackish water; eluates of sediment (freshwater, brackish and sea water); pore water; single substances, diluted in water. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 5667-16, Water quality Sampling
