1、BRITISH STANDARDBS EN1390:2006Wood preservatives Determination of the eradicant action against Hylotrupes bajulus (Linnaeus) larvae Laboratory methodThe European Standard EN 1390:2006 has the status of a British StandardICS 71.100.50g49g50g3g38g50g51g60g44g49g42g3g58g44g55g43g50g56g55g3g37g54g44g3g5
2、1g40g53g48g44g54g54g44g50g49g3g40g59g38g40g51g55g3g36g54g3g51g40g53g48g44g55g55g40g39g3g37g60g3g38g50g51g60g53g44g42g43g55g3g47g36g58BS EN 1390:2006This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 July 2006 BSI 2006ISBN 0 580 49013 0Nationa
3、l forewordThis British Standard is the official English language version of EN 1390:2006. It supersedes DD ENV 1390:1995 which is withdrawn.The UK participation in its preparation was entrusted by Technical Committee B/515, Wood preservatives, to Subcommittee B/515/1, Preservative preconditioning an
4、d biological testing, which has the responsibility to: A list of organizations represented on this subcommittee can be obtained on request to its secretary.Cross-referencesThe British Standards which implement international or European publications referred to in this document may be found in the BS
5、I Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue or of British Standards Online.This publication does not purport to include all the necessary provisions of a contract. Users are responsible for i
6、ts correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep UK interests
7、informed; monitor related international and European developments and promulgate them in the UK.Summary of pagesThis document comprises a front cover, an inside front cover, the EN title page, pages 2 to 20, an inside back cover and a back cover.The BSI copyright notice displayed in this document in
8、dicates when the document was last issued.Amendments issued since publicationAmd. No. Date CommentsEUROPEAN STANDARDNORME EUROPENNEEUROPISCHE NORMEN 1390June 2006ICS 71.100.50 Supersedes ENV 1390:1994 English VersionWood preservatives - Determination of the eradicant actionagainst Hylotrupes bajulus
9、 (Linnaeus) larvae - LaboratorymethodProduits de prservation du bois - Dtermination de lactioncurative contre les larves dHylotrupes bajulus (Linnaeus) -Mthode de laboratoireHolzschutzmittel - Bestimmung der bekmpfeden Wirkunggegenber Larven von Hylotrupes bajulus (Linnaeus) -LaboratoriumsverfharenT
10、his European Standard was approved by CEN on 24 May 2006.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references
11、 concerning such nationalstandards may be obtained on application to the Central Secretariat or to any CEN member.This European Standard exists in three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its o
12、wn language and notified to the Central Secretariat has the same status as the officialversions.CEN members are the national standards bodies of Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France,Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembour
13、g, Malta, Netherlands, Norway, Poland, Portugal, Romania,Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATIONCOMIT EUROPEN DE NORMALISATIONEUROPISCHES KOMITEE FR NORMUNGManagement Centre: rue de Stassart, 36 B-1050 Brussels 2006 CEN All rights of e
14、xploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN 1390:2006: EEN 1390:2006 (E) 2 Contents Page Foreword3 Introduction .4 1 Scope 5 2 Normative references 5 3 Terms and definitions .5 4 Principle5 5 Test materials.6 6 Sampling.7 7 Test specimens7 8 Proced
15、ure .9 9 Validity of the test. 12 10 Expression of results . 12 11 Test report . 13 Annex A (informative) Example of a test report 14 Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus 16 Annex C (informative) Environmental, health and safety precautions within chemical/biolo
16、gical laboratory 19 Bibliography. 20 EN 1390:2006 (E) 3 Foreword This document (EN 1390:2006) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and derived materials”, the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national stand
17、ard, either by publication of an identical text or by endorsement, at the latest by December 2006, and conflicting national standards shall be withdrawn at the latest by December 2006. This document supersedes ENV 1390:1994. Significant technical differences between this standard and ENV 1390:1994 a
18、re as follows: a) introduction of new harmonised specifications for the test specimens used in the diverse biological tests; b) separation of the method according to the expected test periods for fast and slow acting preservatives and for deferred acting preservatives respectively; c) admission of t
19、he terms given in EN 1001-1and the definitions of EN 1001-2; d) introduction of an informative Annex to take account of consideration for minimisation of environmental and health hazards caused by the use of this biological test. According to the CEN/CENELEC Internal Regulations, the national standa
20、rds organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Rom
21、ania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EN 1390:2006 (E) 4 Introduction This document describes a laboratory method of testing which gives a basis for the assessment of the eradicant action of fast and slow acting wood preservatives and of deferred acting wood preser
22、vatives against Hylotrupes bajulus. It allows determination of the lethal effect of a surface application of a preservative product on a population of large larvae previously introduced into the test specimens. The method simulates conditions in practice where a beam is treated, which is only slight
23、ly attacked and where cutting away has not exposed insect tunnels. This represents a severe test of the product. In some particular instances, for example where the preservative is to be used on timbers of large dimensions, laminated beams, blockboard, plywood and other panel products, other test me
24、thods can be used to obtain complementary information on the effectiveness of the eradicant action of a product. Such methods lie outside the scope of this document. This laboratory method provides one criterion by which the value of a product can be assessed. In making this assessment the methods b
25、y which the preservative may be applied should be taken into account. lt is further recommended that results from this test should be supplemented by those from other appropriate tests, and above all by comparison with practical experience. When products that are very active at low concentrations ar
26、e used it is very important to take suitable precautions to isolate and separate, as far as possible, operations involving chemical products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms, extraction
27、 facilities, conditioning chambers and special training for personnel, (see also Annex C for environmental, health and safety precautions). EN 1390:2006 (E) 5 1 Scope This document specifies a method for the determination of the eradicant action of a surface application of a fast and a slow acting w
28、ood preservative product or a deferred acting wood preservative product on timber infested with larvae of Hylotrupes bajulus (Linnaeus). This method is applicable to: organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates, or organic water-dispersible formulat
29、ions, as supplied or as prepared in the laboratory by dilution of concentrates, or water-soluble products, for example, salts. NOTE An ageing procedure cannot be combined with this method. 2 Normative references The following referenced documents are indispensable for the application of this documen
30、t. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN ISO 3696,Water for analytical laboratory use - Specification and test methods (ISO 3696:1987) ISO 835-1:1981, Laboratory glassware - G
31、raduated pipettes - Part 1: General requirements 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample with physical and/or chemical characteristics identical to the volumetric average characteristics of the total volum
32、e being sampled EN 1001-2:2005, 4.71 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to be tested) Adapted from EN 1001-2:2005, 4.83 4 Principle Insertion of larvae of Hylotrupes bajulus into test specimens. After a period of time to allow the larvae to
33、establish themselves in the test specimens, treatment of these test specimens by brushing or pipetting of the test preservative product. After the time necessary for the preservative to act effectively, assessment of the mortality of the larvae compared with that of larvae in untreated control test
34、specimens. EN 1390:2006 (E) 6 5 Test materials 5.1 Biological material 5.1.1 Hylotrupes bajulus (Linnaeus) larvae 5.1.2 Source of larvae. The larvae shall preferably be obtained from cultures reared according to the method described in Annex B. NOTE Larvae can also be taken from naturally infested w
35、ood, in which case they should be transferred into sapwood of pine and stored for at least four weeks under the rearing conditions specified in Annex B. Do not use the larvae in the test if they have not fed normally during this storage period. 5.1.3 Provision of larvae Carefully split or crumble in
36、fested blocks to extract larvae. Keep the larvae separate from one another in glass receptacles for two or three days in the culturing chamber (5.3.1) to check they are healthy. 5.1.4 Choice of larvae Use only healthy larvae in the test. NOTE 1 A healthy larva can be recognized by ivory-white colour
37、, its firm consistency and rounded appearance, and by the absence of wounds or bites, which show up as dark marks. Healthy larvae react to the touch by vigorous movement and attempts to bite. Reject any larvae, which are shrunken or aged which have recently moulted, or which are in a pre-pupal stage
38、. Weigh each larva and place it in a glass receptacle marking the receptacle with the weight of the larva. Make up two groups with the weight ranges: - 51 mg to 100 mg and -101 mg to 150 mg. NOTE 2 Larvae with a mass larger than 150 mg in mass are unsuitable as they can pupate during the course of t
39、he test. 5.2 Products and reagents 5.2.1 Paraffin wax, for sealing the relevant surfaces of specimens to be treated with solutions in which water is the continuous phase. NOTE Paraffin wax with a setting point of 52 C to 54 C has been found suitable. 5.2.2 Gelatine, for sealing the relevant surfaces
40、 of specimens to be treated with solutions in which an organic solvent is the continuous phase. 5.2.3 Water, complying with grade 3 of EN ISO 3696. 5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the pos
41、t-treatment conditioning period that has a toxic effect on the insects. CAUTION Do not use benzene or other solvents which pose a health risk. EN 1390:2006 (E) 7 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, controlled at (28 2) C and at a relative humidity of (70 5) %. 5.3.2 Ventilat
42、ed fume cupboard, in which the specimens are treated with an input air temperature of (20 5) C and a maximum air speed, measured at the input opening with the sash in the approximate operating position, of 0,5 m/s. CAUTION - It is essential to follow safety procedures for handling flammable and toxi
43、c materials. Avoid excessive exposure of operators to solvents or their vapours. 5.3.3 Testing chamber, ventilated and controlled at (21 2) C and at a relative humidity of (75 5) %. 5.3.4 Drill and twist drills, with 3 mm, 4 mm and 5 mm diameters. 5.3.5 Pipettes as specified in ISO 835-1:1981, Class
44、 B - graduated pipette with no waiting time, with a capacity 5 ml and an accuracy of 0,05 ml. 5.3.6 Safety equipment and protective clothing, appropriate for the test product and the test, to ensure the safety of the operator. 5.3.7 Ordinary laboratory equipment, including a balance capable of weigh
45、ing to an accuracy of 1 mg. 5.3.8 Rectangular cover with sides, constructed either of glass, plastics, plywood and of a height not less than 200 mm and with an open face of sufficient size to cover all the treated specimens from a single test. 5.3.9 X-ray apparatus (optional) with tungsten target an
46、d beryllium window, with voltage and current continuously variable in the ranges: -voltage: 10 kV to 50 kV; -current: 0 mA to 15 mA. 5.3.10 Protective gloves 6 Sampling The sample of preservative shall represent the product to be tested. Samples shall be stored and handled in accordance with any wri
47、tten recommendations from the supplier. NOTE For the sampling of preservatives from bulk supplies, the procedure given in EN 212 should be used. 7 Test specimens 7.1 Species of wood The reference species is Scots pine (Pinus sylvestris Linnaeus)1). NOTE Additional tests may be carried out using othe
48、r species but, if so, this should be stated in the test report. 1) In southern European countries the species of pine most frequently infested by Hylotrupes bajulus may be used as an alternative, provided that the suitability of the species for use in the tests specified in this standard has been de
49、monstrated in all aspects (development of larvae, resistance to impregnation, etc.). EN 1390:2006 (E) 8 7.2 Wood quality The wood shall be free from visible cracks, stain, decay, insect damage and other defects. The wood shall not have been water-stored, floated, chemically treated or steamed. The wood shall originate from trees preferably felled in winter. The wood shall not have been stored for more than five years. NOTE 1 Wood that has been kiln dried at temperatures below 60 C may be used. The wood shall be exclusively sapwood containing li
