1、BRITISH STANDARDBS EN ISO 8192:2007Water quality Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidationThe European Standard EN ISO 8192:2007 has the status of a British StandardICS 13.060.30; 13.060.70g49g50g3g38g50g51g60g44g49g42g3g58g44g55g43g50g56g5
2、5g3g37g54g44g3g51g40g53g48g44g54g54g44g50g49g3g40g59g38g40g51g55g3g36g54g3g51g40g53g48g44g55g55g40g39g3g37g60g3g38g50g51g60g53g44g42g43g55g3g47g36g58BS EN ISO 8192:2007This British Standard was published under the authority of the Standards Policy and Strategy Committee on 31 August 2007 BSI 2007ISB
3、N 978 0 580 54191 9National forewordThis British Standard was published by BSI. It is the UK implementation of EN ISO 8192:2007. It supersedes BS EN ISO 8192:1995 (BS 6068-5.7:1987), which are withdrawn. The UK participation in its preparation was entrusted by Technical Committee EH/3, Water quality
4、, to Subcommittee EH/3/5, Biological methods.A list of organizations represented on this committee can be obtained on request to its secretary.ISO 8192:2007, which is a revision of ISO 8192:1986, was instigated following a UK initiative to modify the procedure to facilitate parallel investigations o
5、f inhibition of carbonaceous oxidation and nitrification within the same test.The UK, as a member of CEN, is obliged to publish EN ISO 8192:2007 as a British Standard. However, attention is drawn to the fact that the UK voted against its approval at the final stage of revision of the International S
6、tandard.The reason for this disapproval was that changes introduced to the text at the final pre-approval stage have obscured the meaning of certain sections and introduced ambiguities, contradictions and some minor technical errors. Corrections requested by UK technical committee EH/3/5 at this fin
7、al pre-approval stage were not included in the published ISO Standard and as a consequence the same deficiencies also appear in EN ISO 8192:2007. A list of the changes introduced and possible solutions are given in National annex NA.This publication does not purport to include all the necessary prov
8、isions of a contract. Users are responsible for its correct application.Compliance with a British Standard cannot confer immunity from legal obligations.Amendments issued since publicationAmd. No. Date CommentsEUROPEAN STANDARDNORME EUROPENNEEUROPISCHE NORMEN ISO 8192February 2007ICS 13.060.70 Super
9、sedes EN ISO 8192:1995 English VersionWater quality Test for inhibition of oxygen consumption byactivated sludge for carbonaceous and ammonium oxidation(ISO 8192:2007)Qualit de leau Essai dinhibition de la consommationdoxygne par des boues actives pour loxydation ducarbone et de lammonium (ISO 8192:
10、2007)Wasserbeschaffenheit Bestimmung der Hemmung desSauerstoffverbrauchs von Belebtschlamm nachKohlenstoff- und Ammonium-Oxidation (ISO 8192:2007)This European Standard was approved by CEN on 29 December 2006.CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate t
11、he conditions for giving this EuropeanStandard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such nationalstandards may be obtained on application to the CEN Management Centre or to any CEN member.This European Standard exists in
12、 three official versions (English, French, German). A version in any other language made by translationunder the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as theofficial versions.CEN members are the national standards bodies of
13、 Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland,France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN CO
14、MMITTEE FOR STANDARDIZATIONCOMIT EUROPEN DE NORMALISATIONEUROPISCHES KOMITEE FR NORMUNGManagement Centre: rue de Stassart, 36 B-1050 Brussels 2007 CEN All rights of exploitation in any form and by any means reservedworldwide for CEN national Members.Ref. No. EN ISO 8192:2007: E2 Foreword This docume
15、nt (EN ISO 8192:2007) has been prepared by Technical Committee ISO/TC 147 “Water Quality“ in collaboration with Technical Committee CEN/TC 230 “Water Analysis“, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of
16、an identical text or by endorsement, at the latest by August 2007, and conflicting national standards shall be withdrawn at the latest by August 2007. This document supersedes EN ISO 8192:1995. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following c
17、ountries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia,
18、Spain, Sweden, Switzerland and United Kingdom. Endorsement notice The text of ISO 8192:2007 has been approved by CEN as EN ISO 8192:2007 without any modifications. EN ISO 8192:2007Reference numberISO 8192:2007(E)INTERNATIONAL STANDARD ISO8192Second edition2007-02-01Water quality Test for inhibition
19、of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation Qualit de leau Essai dinhibition de la consommation doxygne par des boues actives pour loxydation du carbone et de lammonium EN ISO 8192:2007ii iiiContents Page Foreword iv Introduction v 1 Scope . 1 2 Normative refere
20、nces . 1 3 Terms and definitions. 1 4 Principle. 2 5 Reagents, media and inoculum. 3 6 Apparatus 4 7 Test environment 5 8 Procedure 5 9 Calculation and expression of results 8 10 Validity of the results 11 11 Test report . 13 Annex A (informative) Examples of measuring units . 14 Annex B (informativ
21、e) Apparatus for culturing nitrifying activated sludge . 16 Annex C (informative) Overview of the test procedure 18 Annex D (informative) Mixtures for the preliminary test 19 Annex E (informative) Example of an inhibition curve . 20 Bibliography . 21 EN ISO 8192:2007Annex NA (informative) UK recomme
22、ndations to clarifiy text of ISO 8192.22iv Foreword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member
23、 body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Elect
24、rotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopte
25、d by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent
26、 rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 8192 was prepared by Technical Committee ISO/TC 147, Water quality, Subcommittee SC 5, Biological methods. This second edition cancels and replaces the first edition (ISO 8192:1986), which has been technica
27、lly revised. EN ISO 8192:2007vIntroduction Information generated by this method for assessing the potential toxicity of substances, mixtures and waste waters to activated sludge may be helpful in estimating the effect of a test material on mixed bacterial communities in the aquatic environment, espe
28、cially in aerobic biological treatment systems. The susceptibility of oxygen uptake by different sub-populations of the bacterial communities to inhibition by chemicals and waste waters is not necessarily uniform and selective effects may profoundly influence the outcome of the test. There are two p
29、rincipal groups of microorganisms contributing to the total oxygen consumption by activated sludge: heterotrophic organisms mainly responsible for the breakdown of carbon-based substrates (carbonaceous oxidation) and autotrophic nitrifying organisms causing the oxidation of ammonium to nitrate (nitr
30、ification). This International Standard may be used to assess the toxicity of substances on total oxygen uptake (i.e. carbonaceous oxidation and nitrification combined) or, by deliberately adding a specific inhibitor of nitrification, also to assess toxicity of substances to the carbonaceous and nit
31、rification components separately. For the determination of the nitrification inhibition with this method, a sufficiently nitrifying activated sludge is required. Indications of nitrification may be investigated further by application of ISO 95094. The user of this method should be aware that particu
32、lar problems could require the specification of additional marginal conditions. The inhibitory effect of a test material may be exerted on both components or it may be exerted predominantly on only one of them. Nitrification is the process more commonly prone to selective inhibition. EN ISO 8192:200
33、7blank ISO 2007 All rights reserved 1Water quality Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation WARNING Persons using this International Standard should be familiar with normal laboratory practice. This International Standard does not purport
34、to address all of the safety problems, if any, associated with its use. It is the responsibility of the user to establish appropriate safety and health practices and to ensure compliance with any national regulatory conditions. IMPORTANT It is absolutely essential that tests conducted according to t
35、his International Standard be carried out by suitably trained staff. 1 Scope This International Standard specifies a method for assessing the inhibitory effect of a test material on the oxygen consumption of activated sludge microorganisms. This method is intended to represent the conditions in biol
36、ogical waste-water treatment plants. It gives information on inhibitory or stimulatory effects after a short exposure (usually 30 min up to 180 min or even more) of the test material on activated sludge microorganisms. This method is applicable for testing waters, waste waters, pure chemicals and mi
37、xtures of chemicals. Concerning the chemicals, the method refers to those which are soluble under the test conditions. Special care is necessary with materials of low water solubility, high volatility and with materials abiotically consuming or producing oxygen. 2 Normative references The following
38、referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO 3696, Water for analytical laboratory use Specification and
39、 test methods 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 activated sludge accumulated biological mass (floc) produced in the treatment of waste water by the growth of bacteria and other microorganisms in the presence of oxygen (ISO 6107-
40、1:2004 3, definition 2) 3.2 concentration of suspended solids of an activated sludge amount of solids obtained by filtration or centrifugation of a known volume of activated sludge and drying at about 105 C to constant mass (ISO 9888:1999 6, definition 3.4) EN ISO 8192:20072 3.3 oxygen consumption r
41、ate uptake of oxygen by activated sludge microorganisms per unit volume of sludge, in unit time NOTE This quantity is expressed in milligrams per litre per hour mg/(lh). 3.4 specific oxygen consumption rate uptake of oxygen by activated sludge microorganisms per unit mass of dry sludge (suspended so
42、lids), in unit time NOTE This quantity is expressed in milligrams per gram per hour mg/(gh). 3.5 inhibition of oxygen consumption decrease of the oxygen consumption rate of an activated sludge plus (a) degradable substance(s) in the presence of the test material, compared with that of a similar mixt
43、ure without test material NOTE 1 This quantity is expressed as a percentage. NOTE 2 In the absence of a substrate, some chemicals (e.g. uncouplers of phosphorylation) can increase oxygen uptake. 3.6 toxic range range of concentration of a test material over which 0 % to 100 % inhibition occurs 3.7 E
44、C50effective concentration of the test material giving a calculated or interpolated inhibition of oxygen consumption of 50 % compared with a blank control 3.8 nitrification oxidation of ammonium compounds by bacteria NOTE Usually the intermediate product is nitrite and the end product is nitrate ISO
45、 6107-1:2004 3,definition 49. 4 Principle In the presence of easily biodegradable substances, activated sludge consumes oxygen at a higher rate than in their absence, depending on, among other factors, the concentration of microorganisms. Addition of a toxic concentration of a test material results
46、in a decrease in the oxygen consumption rate. The rates are measured using an oxygen electrode. The percentage inhibition of the oxygen consumption is estimated by comparison of the rate with that of a control mixture containing no test material. The sensitivity of the activated sludge may be checke
47、d with a suitable reference substance. The inhibition of the oxygen uptake by all sludge microorganisms, heterotrophic microorganisms and the oxidation of ammonium salts by nitrifying microorganisms may be separately expressed from measurements of the rate of uptake in the absence and presence of N-
48、allylthiourea (ATU), a specific inhibitor of the oxidation of ammonium to nitrite by first-stage nitrifiers. The difference between the two oxygen values is due to nitrification and the residual value in the presence of allylthiourea is due to the heterotrophs. Any oxygen consumption due to abiotic
49、processes may be detected by determining the rate in mixtures of the test material, synthetic medium and water, but omitting activated sludge. EN ISO 8192:20073Under certain (rare) circumstances, a test substance with strong reducing properties may cause measurable abiotic oxygen consumption. In such cases, abiotic controls are necessary to discriminate between oxygen uptake by the test substance and microbial respiration. Abiotic controls may be prepared either by omitting the inoculum from test mixtures, or by poisoning the
