1、BRITISH STANDARD BS EN ISO 10707:1998 BS6068-5.16: 1995 Incorporating Amendment No.1 to BS6068-5.16:1995 (renumbers the BS as BS EN ISO 10707:1998) Water quality Evaluation in an aqueous medium of the “ultimate” aerobic biodegradability of organic compounds Methodbyanalysis of biochemical oxygen dem
2、and (closed bottle test) The European Standard EN ISO10707:1997 has the status of a British Standard ICS 13.060.01BSEN ISO 10707:1998 This British Standard, having been prepared under the directionof the Health and Environment Sector Board (H/-), was published under the authorityof the Standards Boa
3、rdand comes into effect on 15March1995 BSI 04-1999 The following BSI references relate to the work on this standard: Committee reference EPC/44 Draft for comment 93/506821 DC ISBN 0 580 23944 6 Committees responsible for this British Standard The preparation of this British Standard was entrusted to
4、 Technical Committee EPC/44, Water quality, upon which the following bodies were represented: Association of Consulting Scientists British Association for Chemical Specialists British Gas plc Chemical Industries Association Convention of Scottish Local Authorities Department of the Environment (Wate
5、r Directorate) Department of the Environment for Northern Ireland Department of Trade and Industry (Laboratory of the Government Chemist) Electricity Association Industrial Water Society Institute of Gas Engineers Institution of Water Officers Institution of Water and Environmental Management Nation
6、al Rivers Authority Royal Institute of Health and Hygiene Royal Society of Chemistry Scottish Association of Directors of Water and Sewerage Services Soap and Detergent Industry Association Water Companies Association Water Research Centre Water Services Association of England and Wales The followin
7、g bodies were also represented in the drafting of the standard, through subcommittees and panels: BLWA Ltd. (The Association of the Laboratory Supply Industry) Department of Economic Development (Northern Ireland) Department of Health Freshwater Biological Association Scottish National Heritage Amen
8、dments issued since publication Amd. No. Date Comments 9903 March 1998 see national forewordBSEN ISO 10707:1998 BSI 04-1999 i Contents Page Committees responsible Inside front cover National foreword ii Foreword 2 1 Scope 3 2 Normative references 3 3 Definitions 3 4 Principle 4 5 Test environment 4
9、6 Reagents 4 7 Apparatus 5 8 Procedure 5 9 Calculation and expression of results 6 10 Validity of the test 7 11 Test report 7 Annex A (informative) Determination of the theoretical oxygen demand (ThOD) 8 Annex B (informative) Determination of the chemical oxygen demand (COD) 8 Annex C (informative)
10、Correction of oxygen uptake for interference by nitrification 9 Annex ZA (normative) Normative references to international publications with their relevant European publications 10BSEN ISO 10707:1998 ii BSI 04-1999 National foreword This British Standard is the English language version of EN ISO1070
11、7:1997. It is identical with ISO10707:1994. The UK participation in its preparation was entrusted by Technical Committee EH/3, Water quality, to Subcommittee EH/3/5, Biological methods, which has the responsibility to: aid enquirers to understand the text; present to the responsible international co
12、mmittee any enquiries on interpretation, or proposals for change, and keep UK interests informed; monitor related international and European developments and promulgate them in the UK. A list of organizations represented on this subcommittee can be obtained on request to its secretary. BS EN ISO1070
13、7 is one of a series of standards on water quality, others of which have been, or will be, published as Sections of BS6068. This standard has therefore been given the secondary identifier BS6068-5.16. The various Sections of BS6068 are comprised within Parts1 to7, which, together with Part0, are lis
14、ted below. Part 0: Introduction; Part 1: Glossary; Part 2: Physical, chemical and biochemical methods; Part 3: Radiological methods; Part 4: Microbiological methods; Part 5: Biological methods; Part 6: Sampling; Part 7: Precision and accuracy. Amendment No.1 The contents page and national foreword h
15、ave been amended to reflect the implementation of EN ISO10707:1997 and the EN title page, EN foreword and Annex ZA have been introduced. Cross-references Attention is drawn to the fact that CEN and CENELEC Standards normally include an annex which lists normative references to international publicat
16、ions with their corresponding European publications. The British Standards which implement these international or European publications may be found in the BSIStandards catalogue under the section entitled “International Standards Correspondence Index”, or using the “Find” facility of the BSI Standa
17、rds Electronic Catalogue. A British Standard does not purport to include all the necessary provisions of a contract. Users of British Standards are responsible for their correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of page
18、s This document comprises a front cover, an inside front cover, pages i and ii, theEN ISO title page, pages 2 to10 and a back cover. This standard has been updated (see copyright date) and may have had amendments incorporated. This will be indicated in the amendment table on theinside front cover.EU
19、ROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO10707 November 1997 ICS13.060.40 Descriptors: See ISO document English version Water quality Evaluation in an aqueous medium of the “ultimate” aerobic biodegradability of organic compounds Method by analysis of biochemical oxygen demand (closedbo
20、ttle test) (ISO10707:1994) Qualit de leau Evaluation en milieu aqueux de la biodgradabilit arobie “ultime” des composs organiques Mthode par analyse de la demande biochimique en oxygne (essaienfiole ferme (ISO10707:1994) Wasserbeschaffenheit Bestimmung der vollstndigen aeroben biologischen Abbaubark
21、eit organischer Stoffe in einem wrigen Medium Verfahren mittels Bestimmung des biochemischen Sauerstoffbedarfs (Geschlossener Flaschentest) (ISO10707:1994) This European Standard was approved by CEN on30 October1997. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which sti
22、pulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the Central Secretariat or to any CEN member. This European Standard
23、exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the Central Secretariat has the same status as the official versions. CEN members are the national standards
24、 bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and United Kingdom. CEN European Committee for Standardization Comit Europen de Normalisation Europisches Komitee fr
25、Normung Central Secretariat: rue de Stassart 36, B-1050 Brussels 1997 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members Ref. No. EN ISO10707:1997 EENISO 10707:1997 BSI 04-1999 2 Foreword The text of the International Standard from Technical Commi
26、ttee ISO/TC147, Water quality, of the International Organization for Standardization (ISO) has been taken over as a European Standard by Technical Committee CEN/TC230, Water analysis, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, ei
27、ther by publication of an identical text or by endorsement, at the latest by May1998, and conflicting national standards shall be withdrawn at the latest by May1998. According to CEN/CENELEC Internal Regulations, the following countries are bound to implement this European Standard: Austria, Belgium
28、, CzechRepublic, Denmark, Finland, France, Germany, Greece, Iceland, Ireland, Italy, Luxembourg, Netherlands, Norway, Portugal, Spain, Sweden, Switzerland and the United Kingdom. NOTENormative references to International Standards are listed in Annex ZA (normative).ENISO10707:1997 BSI 04-1999 3 WARN
29、ING SAFETY PRECAUTIONS Activated sludge and sewage may contain potentially pathogenic organisms. Therefore appropriate precautions should be taken when handling them. Toxic test compounds and those whose properties are unknown should be handled with care. 1 Scope This International Standard specifie
30、s a method, by analysis of biochemical oxygen demand, for the evaluation in an aqueous medium of the “ultimate” biodegradability of organic compounds at a given concentration by aerobic microorganisms. The conditions described in this International Standard do not necessarily always correspond to th
31、e optimal conditions for allowing the maximum value of bio-degradation to occur. The method applies to all organic compounds which are sufficiently water soluble to prepare a stock solution or poorly water soluble when using special dosing techniques. Due to the low concentration of test compound at
32、 the beginning of the test, normally no special precautions for the toxicity of the test compound to the microorganisms of the inoculum is necessary; if required a parallel inhibition test can be performed. 2 Normative references The following standards, contain provisions which, through reference i
33、n this text, constitute provisions of this International Standard. At the time of publication, the editions indicated were valid. All standards are subject to revision, and parties to agreements based on this International Standard are encouraged to investigate the possibility of applying the most r
34、ecent editions of the standards indicated below. Members of IEC and ISO maintain registers of currently valid International Standards. ISO5813:1983, Water quality Determination of dissolved oxygen Iodometric method. ISO5814:1990, Water quality Determination of dissolved oxygen Electrochemical probe
35、method. ISO6060:1989, Water quality Determination of the chemical oxygen demand. ISO9887:1992, Water quality Evaluation of the aerobic biodegradability of organic compounds in an aqueous medium Semi-continuous activated sludge method (SCAS). ISO9888:1991, Water quality Evaluation of the aerobic biod
36、egradability of organic compounds in an aqueous medium Static test (Zahn-Wellens method). ISO10304-2: 1) , Water quality Determination of dissolved anions by liquid chromatography of ions Part2: Determination of bromide, chloride, nitrate, nitrite, orthophosphate and sulfite in waste water. ISO10634
37、 1) , Water quality Guidance for the evaluation in an aqueous medium of the “ultimate” biodegradability of poorly soluble organic compounds. 3 Definitions For the purposes of this International Standard, the following definitions apply. 3.1 ultimate biodegradation the level of degradation achieved w
38、hen the test compound is totally utilized by microorganisms resulting in the production of carbon dioxide, water, mineral salts and new microbial cellular constituents (biomass) 3.2 biochemical oxygen demand (BOD) the mass concentration of dissolved oxygen consumed under specified conditions by the
39、biological oxidation of organic and/or inorganic matter in water and is expressed in this case as milligrams of oxygen uptake per milligram or gram of test compound 3.3 chemical oxygen demand (COD) the amount of oxygen consumed during oxidation of a test compound with hot, acidic dichromate. It prov
40、ides a measure of the amount of oxidizable matter present and is expressed in this case as milligrams of oxygen consumed per milligram or gram of test compound 3.4 theoretical oxygen demand (ThOD) the total amount of oxygen required to oxidize a chemical completely. It is calculated from the molecul
41、ar formula and is expressed in this case as milligrams of oxygen required per milligram or gram of test compound 1) To be published.EN ISO 10707:1997 4 BSI 04-1999 3.5 pre-exposure (or pre-adaptation) the pre-incubation of an inoculum in the presence of the test compound, with the aim of enhancing t
42、he ability of the inoculum to degrade the test compound. If the aim is achieved, the inoculum is said to be adapted 3.6 pre-conditioning (or pre-acclimatization) the pre-incubation of an inoculum under the conditions of the test in the absence of the test compound, to improve the performance of the
43、test 4 Principle A solution of the organic test compound in a mineral medium as the sole source of carbon and energy is inoculated with a relatively small number of micro-organisms from a mixed population and kept in completely full, closed bottles in the dark at a constant temperature. Biodegradati
44、on is followed by analysis of dissolved oxygen over a period of28d. The amount of oxygen taken up by the test chemical (BOD), corrected for uptake by the blank inoculum run in parallel, is expressed as percentage of ThOD or COD. 5 Test environment Incubation shall take place in the dark in an enclos
45、ure which is maintained at a constant temperature (within 1 C) between20C and25C. 6 Reagents Use only reagents of recognized analytical grade. 6.1 Water Distilled or deionized water free from inhibitory concentrations of toxic substances containing less than10% of the initial DOC content introduced
46、by the compounds to be tested. For each series of tests, use only one batch of water. 6.2 Test medium 6.2.1 Composition 6.2.1.1 Solution a) Dissolve the ingredients in water(6.1) and make up to1000ml. NOTE 1The correct composition of the medium can be checked by the measurement of the pH-value, whic
47、h should be7.4. 6.2.1.2 Solution b) Dissolve22,5g of magnesium sulfate heptahydrate (MgSO 4 .7H 2 O) in water(6.1) and dilute to1000ml. 6.2.1.3 Solution c) Dissolve27,5g of anhydrous calcium chloride (CaCl 2 ) or36,4g of calcium chloride dihydrate (CaCl 2 .2H 2 O) in water(6.1) and dilute to1000ml.
48、6.2.1.4 Solution d) Dissolve0,25g of iron(III) chloride hexahydrate (FeCl 3 .6H 2 O) in water(6.1) and dilute to1000ml. In order to avoid having to prepare this solution immediately before use, add one drop of concentrated hydrochloric acid (HCl) or0,4g of ethylenediaminetetraacetic acid (EDTA) (dis
49、odiumsalt) per litre. 6.2.2 Preparation of the test medium For1 litre of test medium, add1ml of each of the solutions a) to d)(6.2.1) to about500ml of water(6.1) and adjust the volume to1000ml. Strongly aerate the test medium for at least20min. Carry out each series of tests with test medium derived from the same batch. Generally, the medium is ready for use after standing for20h at the test temperature. Determine the concentration of dissolved oxygen for control purposes; the value should be
