1、BSI Standards PublicationBS EN ISO 20743:2013Textiles Determination ofantibacterial activity of textileproducts (ISO 20743:2013)BS EN ISO 20743:2013 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of EN ISO20743:2013. It supersedes BS EN ISO 20743:2007 which is withdr
2、awn.The UK participation in its preparation was entrusted to TechnicalCommittee TCI/80, Chemical testing of textiles.A list of organizations represented on this committee can beobtained on request to its secretary.This publication does not purport to include all the necessaryprovisions of a contract
3、. Users are responsible for its correctapplication. The British Standards Institution 2013. Published by BSI StandardsLimited 2013ISBN 978 0 580 75226 1ICS 07.100.99; 59.080.01Compliance with a British Standard cannot confer immunity fromlegal obligations.This British Standard was published under th
4、e authority of theStandards Policy and Strategy Committee on 31 July 2013.Amendments issued since publicationDate Text affectedEUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 20743 July 2013 ICS 07.100.99; 59.080.01 Supersedes EN ISO 20743:2007English Version Textiles - Determination of ant
5、ibacterial activity of textile products (ISO 20743:2013) Textiles - Dtermination de lactivit antibactrienne des produits textiles (ISO 20743:2013) Textilien - Bestimmung der antibakteriellen Wirkung antibakteriell behandelter Erzeugnisse (ISO 20743:2013) This European Standard was approved by CEN on
6、 22 June 2013. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may b
7、e obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to
8、the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, I
9、reland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Aven
10、ue Marnix 17, B-1000 Brussels 2013 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 20743:2013: EBS EN ISO 20743:2013EN ISO 20743:2013 (E) 3 Foreword This document (EN ISO 20743:2013) has been prepared by Technical Committee ISO
11、/TC 38 “Textiles“ in collaboration with Technical Committee CEN/TC 248 “Textiles and textile products” the secretariat of which is held by BSI. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by Janu
12、ary 2014, and conflicting national standards shall be withdrawn at the latest by January 2014. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent
13、 rights. This document supersedes EN ISO 20743:2007. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finlan
14、d, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO
15、 20743:2013 has been approved by CEN as EN ISO 20743:2013 without any modification. BS EN ISO 20743:2013ISO 20743:2013(E) ISO 2013 All rights reserved iiiContents PageForeword ivIntroduction v1 Scope . 12 Normative references 13 Terms and definitions . 14 Safety precaution . 25 Apparatus . 26 Reagen
16、ts and culture media . 46.1 Water . 46.2 Tryptone soya broth (TSB) . 46.3 Tryptone soya agar (TSA) 46.4 Agar for transfer 56.5 Nutrient broth (NB) 56.6 Peptone salt solution . 56.7 Physiological saline . 56.8 SCDLP medium . 66.9 Dilution buffer for shake-out bacterial suspension. 66.10 Neutralizing
17、solution . 66.11 Enumeration agar (EA) 76.12 Agar for printing 76.13 Cryoprotective solution for bacterial species . 76.14 Stock solution of ATP standard reagent 76.15 Buffer solution for ATP luminescent reagent . 86.16 ATP luminescent reagent . 86.17 ATP extracting reagent . 86.18 ATP eliminating r
18、eagent . 86.19 SCDLP or other medium for preparing ATP reference solution . 96.20 Shake-out physiological saline 97 Reference strains . 97.1 Strains . 97.2 Storage of strains . 108 Test procedures 118.1 Absorption method (see Annex E) 118.2 Transfer method (see Annex E) . 158.3 Printing method (see
19、Annex E) 189 Test report 22Annex A (normative) Strain numbers 23Annex B (normative) Shaking method 24Annex C (normative) Quantitative measurement by plate count method 25Annex D (normative) Quantitative measurement by luminescence method 26Annex E (informative) Testing examples .28Annex F (informati
20、ve) Efficacy of antibacterial activity 31Bibliography .32BS EN ISO 20743:2013ISO 20743:2013(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried
21、 out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO
22、 collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different a
23、pproval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2. www.iso.org/directivesAttention is drawn to the possibility that some of the elements of this document may be the subj
24、ect of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received. www.iso.org/patentsAny trade name
25、used in this document is information given for the convenience of users and does not constitute an endorsement.The committee responsible for this document is ISO/TC 38, Textiles.This second edition cancels and replaces the first edition (ISO 20743:2007), which has been technically revised.iv ISO 201
26、3 All rights reservedBS EN ISO 20743:2013ISO 20743:2013(E)IntroductionSpeciality products of antibacterial-treated textiles have been introduced in the market and are expanding year by year in various applications. Those textiles certainly meet the consumers requirement to seek prevention and protec
27、tion from the negative effects caused by bacteria and to secure the quality of life.In this situation, the test methods to determine the antibacterial activity for antibacterial textile products were expected to be established in order to address the substantial need for an International Standard.Th
28、e test method for antibacterial activity was developed as ISO 20645 which was a qualitative test method. There are no testing standards for the quantitative method which gives more objective information for the antibacterial activity of the textile products.There are several practical test methods t
29、o determine the quantitative antibacterial activity specified in this International Standard. The test methods are composed of 2 major steps, such as inoculation of bacteria and quantitative measurement of bacteria.The methods for the inoculation of bacteria specified in this International Standard
30、are the absorption method, transfer method and printing method.The methods of the quantitative measurement of bacteria specified in this International Standard are colony plate count method and ATP luminescence methods.Although there are 6 ways for the combination of inoculation methods and quantita
31、tive measurements to execute this test, the choice of the ways depends on the users availability and consensus between the concerned parties. ISO 2013 All rights reserved vBS EN ISO 20743:2013BS EN ISO 20743:2013Textiles Determination of antibacterial activity of textile products1 ScopeThis Internat
32、ional Standard specifies quantitative test methods to determine the antibacterial activity of all antibacterial textile products including nonwovens.This International Standard is applicable to all textile products, including cloth, wadding, thread and material for clothing, bedclothes, home furnish
33、ings and miscellaneous goods, regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) or the method of application (built-in, after-treatment or grafting).Based on the intended application and on the environment in which the textile product is to be used and also
34、 on the surface properties of the textile properties, the user can select the most suitable of the following three inoculation methods on determination of antibacterial activity:a) absorption method (an evaluation method in which the test bacterial suspension is inoculated directly onto specimens);b
35、) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto specimens);c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto specimens).The colony plate count method and the ATP (ATP = Adenosine Tri
36、-phosphate) luminescence method are also specified for measuring the enumeration of bacteria.2 Normative referencesThe following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest editi
37、on of the referenced document (including any amendments) applies.ISO 6330, Textiles Domestic washing and drying procedures for textile testing3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.3.1control fabricfabric used to validate the growth condit
38、ion of test bacteria and validate the testNote 1 to entry: The same fabric as the fabric to be tested but without antibacterial treatment or a 100 % cotton fabric without fluorescent brighteners or other finish can be used.3.2antibacterial agentproduct designed to prevent or mitigate the growth of b
39、acteria, to reduce the number of bacteria or to kill bacteriaINTERNATIONAL STANDARD ISO 20743:2013(E) ISO 2013 All rights reserved 1BS EN ISO 20743:2013ISO 20743:2013(E)3.3antibacterial finishtreatment designed to prevent or mitigate the growth of bacteria, to reduce the number of bacteria or to kil
40、l bacteria3.4antibacterial activityactivity of an antibacterial finish used to prevent or mitigate the growth of bacteria, to reduce the number of bacteria or to kill bacteria3.5plate count methodmethod in which the number of bacteria present after incubation is calculated by counting the number of
41、colonies according to a ten-time dilution methodNote 1 to entry: The results are expressed in “CFU (Colony Forming Unit)”.3.6luminescence methodmethod in which the amount of ATP contained in bacterial cells is measuredNote 1 to entry: The results are expressed in “moles of ATP”.3.7neutralizerchemica
42、l agents used to inactivate, neutralize or quench the antibacterial properties of antibacterial agents4 Safety precautionThe test methods specified in this International Standard require the use of bacteria.These tests should be carried out by persons with training and experience in the use of micro
43、biological techniques.Appropriate safety precautions should be observed with due consideration given to country-specific regulations.5 ApparatusUsual laboratory apparatus and, in particular, the following.5.1 Spectrophotometer, capable of measuring at a 620 nm to 660 nm wavelength, or McFarlands nep
44、helometer.5.2 Incubator, capable of maintaining a constant temperature of 37 C 2 C.5.3 Water baths, one capable of maintaining a constant temperature of 46 C 2 C and another capable of maintaining a temperature of 70 C to 90 C.5.4 Mixer, producing a vortex shaking action.5.5 Stomacher, capable of sp
45、eeds of 6 blows per second to 8 blows per second, with the corresponding disposable containers.5.6 Clean bench, for microbial test.5.7 Washing machine, in accordance with the specifications of ISO 6330.2 ISO 2013 All rights reservedBS EN ISO 20743:2013ISO 20743:2013(E)5.8 Humidity chamber, tropical
46、chamber or other container capable of maintaining a high-humidity more than 70 %RH atmospheric condition.5.9 Luminescence photometer, capable of measuring ATP of 1012mol/l to 107mol/l at 300 nm to 650 nm with a luminescence-measuring reagent.5.10 Printing apparatus, capable of applying a 4 N load to
47、 a test specimen and rotating the specimen 180 in one direction for a period of 3,0 s.5.11 Refrigerator, capable of maintaining a temperature of between 2 C and 8 C.5.12 Freezers, one adjustable to a temperature below 70 C and another to a temperature below 20 C.5.13 Balance, which can be read to th
48、e nearest 0,01 g.5.14 Filtering apparatus, consisting of an upper container equipped with a membrane filter and a lower container equipped with a suction opening.5.15 Pipette, having the most suitable volume for each use, with a tip made of glass or plastic, and with a tolerance of 0,5 % or less.5.1
49、6 Vials, 30 ml glass bottles, with screw openings, polytetrafluoroethylene or silicone packing and caps made of polypropylene, polycarbonate or another suitable material.5.17 Petri dishes, that have been sterilized, made of glass or plastic, in diameter sizes of 90 mm to 100 mm or 55 mm to 60 mm.5.18 Glass rod, with a diameter of approximately 18 mm.5.19 Anti-bumping granules (glass beads), with a diameter of 3 mm to 4 mm.5.20 Erlenmeyer flask, of capacity 100 ml.5.21 Cutting template, made of a sterilizable material (stainless steel
