1、BSI Standards PublicationSoil quality Risk-based petroleum hydrocarbonsPart 2: Determination of aliphatic and aromatic fractions of semi-volatile petroleum hydrocarbons using gas chromatography with flame ionization detection (GC/FID)PD CEN ISO/TS 16558-2:2015National forewordThis Published Document
2、 is the UK implementation of CEN ISO/TS 16558-2:2015.The UK participation in its preparation was entrusted to TechnicalCommittee EH/4, Soil quality.A list of organizations represented on this committee can be obtained onrequest to its secretary.This publication does not purport to include all the ne
3、cessary provisions ofa contract. Users are responsible for its correct application. The British Standards Institution 2015.Published by BSI Standards Limited 2015ISBN 978 0 580 76263 5ICS 13.080.10Compliance with a British Standard cannot confer immunity fromlegal obligations.This Published Document
4、 was published under the authority of theStandards Policy and Strategy Committee on 30 September 2015.Amendments/corrigenda issued since publicationDate Text affectedPUBLISHED DOCUMENTPD CEN ISO/TS 16558-2:2015TECHNICAL SPECIFICATION SPCIFICATION TECHNIQUE TECHNISCHE SPEZIFIKATION CEN ISO/TS 16558-2
5、 September 2015 ICS 13.080.10 English Version Soil quality - Risk-based petroleum hydrocarbons - Part 2: Determination of aliphatic and aromatic fractions of semi-volatile petroleum hydrocarbons using gas chromatography with flame ionization detection (GC/FID) (ISO/TS 16558-2:2015) Qualit du sol - H
6、ydrocarbures de ptrole risque - Partie 2: Dtermination des fractions aliphatiques et aromatiques des hydrocarbures de ptrole semi-volatiles par chromatographie en phase gazeuse avec dtection dionisation de la flamme (ISO/TS 16558-2:2015) Bodenbeschaffenheit - Minerallkohlenwasserstoffe fr die Risiko
7、beurteilung - Teil 2: Teil 2: Bestimmung aliphatischer und aromatischer Fraktionen schwerflchtiger Minerallkohlenwasserstoffe mittels Gaschromatographie mit Flammenionisationsdetektion (GC/FID) (ISO/TS 16558-2:2015) This Technical Specification (CEN/TS) was approved by CEN on 13 July 2015 for provis
8、ional application. The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit their comments, particularly on the question whether the CEN/TS can be converted into a European Standard. CEN members are required to announce
9、 the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS available promptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS) until the final decision about the possible conversion of the C
10、EN/TS into an EN is reached. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta
11、, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey andUnited Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2015 CEN A
12、ll rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. CEN ISO/TS 16558-2:2015 EPD CEN ISO/TS 16558-2:2015CEN ISO/TS 16558-2:2015 (E) 2 Contents Page European foreword . 3 PD CEN ISO/TS 16558-2:2015CEN ISO/TS 16558-2:2015 (E) 3 European foreword
13、This document (CEN ISO/TS 16558-2:2015) has been prepared by Technical Committee ISO/TC 190 “Soil quality” in collaboration with Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN. Attention is drawn to the possibility that some of the elements of this
14、 document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to announce this Technical Speci
15、fication: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia
16、, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO/TS 16558-2:2015 has been approved by CEN as CEN ISO/TS 16558-2:2015 without any modification. PD CEN ISO/TS 16558-2:2015ISO/TS 16558-2:2015(E)Foreword ivIntroduction v1 Scope . 12 Normative references 13
17、Terms and definitions . 24 Interferences 25 Principle 26 Reagents 37 Apparatus . 48 Sampling, sample conservation, and pretreatment . 59 Procedure. 59.1 Blank 59.2 Extraction 69.2.1 Total petroleum hydrocarbons 69.2.2 Split into aliphatic and aromatic fractions 69.3 Determination by gas chromatograp
18、hy . 79.3.1 Test of the performance of the gas chromatographic system . 79.3.2 Repeatability test . 79.3.3 Calibration . 79.3.4 Validity check of the calibration function . 79.3.5 Measurement 79.3.6 Integration . 79.3.7 Calculation of the total petroleum hydrocarbons 99.3.8 Calculation of the indivi
19、dual extractable fractions . 99.4 Quality control . 99.4.1 Suitability check of the split procedure . 910 Expression of results .1011 Test report 10Annex A (informative) Examples of gas chromatograms of total extractable petroleum hydrocarbon and aliphatic and aromatic fractions in a standard soluti
20、on and in soil samples 11Annex B (informative) Determination of the boiling range of mineral oil hydrocarbons from the gas chromatogram .14Annex C (informative) Information on split of aliphatic and aromatic fractions using silicagel 16Bibliography .20 ISO 2015 All rights reserved iiiContents PagePD
21、 CEN ISO/TS 16558-2:2015ISO/TS 16558-2:2015(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each memb
22、er body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Ele
23、ctrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types
24、of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www.iso.org/directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be hel
25、d responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www.iso.org/patents).Any trade name used in this document is information
26、 given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the WTO principles in the Technical Barriers to Trade (TBT) see the foll
27、owing URL: Foreword - Supplementary informationThe committee responsible for this document is ISO/TC 190, Soil quality, Subcommittee SC 3, Chemical methods and soil characteristics.ISO 16558 consists of the following parts, under the general title Soil quality Risk-based petroleum hydrocarbons: Part
28、 1: Determination of aliphatic and aromatic fractions of volatile petroleum hydrocarbons using gas chromatography (static headspace method) Part 2: Determination of aliphatic and aromatic fractions of semi-volatile petroleum hydrocarbons using gas chromatography with flame ionization detection (GC/F
29、ID) Technical Specificationiv ISO 2015 All rights reservedPD CEN ISO/TS 16558-2:2015ISO/TS 16558-2:2015(E)IntroductionISO 11504 establishes a basis for the choice of fractions and individual compounds when carrying out analysis for petroleum hydrocarbons in soils and soil-like materials including se
30、diments. It provides guidance for the appropriate use of the analytical results in risks assessment. This part of ISO 16558 specifies methods for the quantitative determination of the appropriate fractions of aliphatic and aromatic compounds. The methods described in this part of ISO 16558 are based
31、 on existing standards mineral oil (ISO 16703) and volatile hydrocarbons (ISO 22155). The general use and relation between the two different parts of ISO 16558 are given in Figure 1.Keya Florisilbclean-up: Only to be applied in case the test according to ISO 16703 is carried out. If the aliphatic an
32、d aromatic fractions have to be analysed, Florisil clean-up is not to be carried out. Florisil is a trade name for a prepared diatomaceous substance, mainly consisting of anhydrous magnesium silicate.b Florisil is an example of a suitable product available commercially. This information is given for
33、 the convenience of users of this part of ISO 16558 and does not constitute an endorsement by ISO of this product.Figure 1 Use of different analytical International Standards during risk assessment of petroleum hydrocarbons ISO 2015 All rights reserved vPD CEN ISO/TS 16558-2:2015Soil quality Risk-ba
34、sed petroleum hydrocarbons Part 2: Determination of aliphatic and aromatic fractions of semi-volatile petroleum hydrocarbons using gas chromatography with flame ionization detection (GC/FID)1 ScopeThis part of ISO 16558 specifies a method for the quantitative determination of the total extractable s
35、emi-volatile, aliphatic, and aromatic fractions of petroleum hydrocarbon content in field moist soil samples by gas chromatography.The results of the test carried out can be used for risk assessment studies related to contaminations with petroleum hydrocarbons.This part of ISO 16558 provides a metho
36、d applicable to petroleum hydrocarbon contents from about 100 mg/kg soil expressed as dry matter for the whole aliphatic fraction C10to C40,as well as the aromatic fraction C10to C40. For sub-fractions, lower limits of determination can be reached.If lower detection limits are required, large volume
37、 injection can be used or concentration of the final extract can be carried out.NOTE 1 Low concentrations of aliphatic and aromatic compounds can be found in natural uncontaminated organic rich soils like peat soils.With this method, all hydrocarbons with a boiling range of 174 C to 525 C, n-alkanes
38、 between C10H22to C40H82, isoalkanes, cycloalkanes, alkyl benzenes, and alkyl naphthalenes and polycyclic aromatic compounds are determined as total extractable semi-volatile petroleum hydrocarbons C10to C40; besides that, semi-volatile aliphatic and aromatic fractions are specified.For the determin
39、ation of volatile aliphatic and aromatic fractions of petroleum hydrocarbons in soil samples, see ISO 16558-1.NOTE 2 The sub-fractions proposed in this part of ISO 16558 have shown to be suitable for risk assessment studies. However, other sub-fractions between C10H22to C40H82can also be determined
40、in conformity with this part of ISO 16558.On the basis of the peak pattern of the gas chromatogram and of the boiling points of the individual n-alkanes listed in Annex B, the approximate boiling range of the mineral oil and some qualitative information on the composition of the contamination can be
41、 achieved.2 Normative referencesThe following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including
42、any amendments) applies.ISO 8466-1, Water quality Calibration and evaluation of analytical methods and estimation of performance characteristics Part 1: Statistical evaluation of the linear calibration functionISO 10381-1, Soil quality Sampling Part 1: Guidance on the design of sampling programmesTE
43、CHNICAL SPECIFICATION ISO/TS 16558-2:2015(E) ISO 2015 All rights reserved 1PD CEN ISO/TS 16558-2:2015ISO/TS 16558-2:2015(E)ISO 11465, Soil quality Determination of dry matter and water content on a mass basis Gravimetric methodISO 14507, Soil quality Pretreatment of samples for determination of orga
44、nic contaminantsISO 16703, Soil quality Determination of content of hydrocarbon in the range C10 to C40 by gas chromatographyISO 18512, Soil quality Guidance on long and short term storage of soil samples3 Terms and definitionsFor the purposes of this document, the following terms and definitions ap
45、ply.3.1total extractable semi-volatile petroleum hydrocarbon content by gas chromatographysum of compounds extractable with acetone/n-heptane (2+1) that can be detected with a flame ionization detector and chromatographed on a non-polar capillary column with retention times between those of n-decane
46、 (C10H22) and n-tetracontane (C40H82)Note 1 to entry: Substances that comply with that definition are mainly long chain or branched aliphatic, alicyclic, lower polycyclic, or alkyl substituted aromatic hydrocarbons.3.2semi-volatile aliphatic fraction of petroleum hydrocarbonsfraction of the total se
47、mi-volatile petroleum hydrocarbons which are eluted with pentane, hexane, or heptane after adsorption on silicagelNote 1 to entry: This is a method defined in this part of ISO 16558. It is unknown and unpredictable if the same compounds will elute from the silicagel with other solvents.3.3semi-volat
48、ile aromatic fraction of petroleum hydrocarbonsfraction of the total semi-volatile petroleum hydrocarbons which are eluted with dichloromethane or with a 1:1 mixture of dichloromethane and n-heptane after adsorption on silicagelNote 1 to entry: This is a method defined in this part of ISO 16558. It
49、is unknown and unpredictable if the same compounds will elute from the silicagel with other solvents.4 InterferencesCompounds not related to petroleum hydrocarbon contaminations with a boiling point between C10and C40(e.g. halogenated hydrocarbons) might interfere with the determination.5 PrincipleA known amount of the homogenized soil sample is extracted by mechanical shaking or sonication with acetone/n-heptane. The organic layer is separated and washed twice with water. An aliquot of the extract is analysed
