1、 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS 2006 10 31 http:/www.kats.go.krKS I ISO 16712 KS I ISO 16712： 2006 (2011 )I ISO 16712： 2006 : ( ) ( ) KIST ( ) : (http:/www.standard.go.kr) ： ：2006 10 31 ：2011 12 26 2011-0623 ： ： ( 02- 509-7270) (http:/www.kats.go.kr). 10 5 , . ICS 13.080.30 KS I ISO 16712
2、： 2006(2011 ) Water quality Determination of acute toxicity of marine or estuarine sediment to amphipods 2005 1 ISO 16712 Water quality Determination of acute toxicity of marine or estuarine sediment to amphipods . 1. a), b), c) 10 . a) b) , , c) 2. 10 . (%) , 175 mL 1 L . . 3. 3.1 , , . 3.2 5001 00
3、0 lx . , 24 39. 4. 4.1 4.1.1 B , . 18 . 4.1.2 , B . B ( ) . 4.1.3 . (background levels) I ISO 16712： 2006 2 11, 12, 48.4.1.4 , (1), . , (1 cm) . . ( B ), 85 % . / , . / . 4 . . , ( , , ), ( ). (total volatile residue), (AVS)/ (SEM), , , , , , , 3, 18. pH, , . ( peepers) ( ) 1, 19, 35. , 175 mL (6.3
4、). ( 2 cm) . 3 . 1 . . . (46 mm , ) 3, 18. 900 mL ( ) . . . I ISO 16712： 2006 6 , , , ( 1, 18, 20 ). 9. . 7. 7.1 , , , . . , 1 3, 18. ( ) , . , 24 cm 2 . . 7.2 20 ( , 20 ). 0.5 mm ( . B ) (4.1.5 ) . , 150 mL . , 1 2 20 . . . 6.4 . . 900 mL ( ), , , . . , , 1 . 1 . 7.3 ( ) ( ). 10 . ( B ). 900 mL 2 c
5、m (175 mL) . . 5001 000 lx . , 5 3 . , 20 3 . I ISO 16712： 2006 7 7.4 , . 1 , , pH . . . . 8, 39. , 1 pH . , 1, 19. ( ) AVS/SEM . . , , , . 10 , 0.5 mm . (2 ) . . ( .). 7.5 10 ( ) . . 7.6 10 3, 18, 39, 45, . 2 cm ( B ) 0.5 mm ( 2 cm) . 1 . ( Monoporeia affinis), . , 10 ( ) ( ) . 7.7 85 % 10 80 % . 8
6、. 8.1 . , (Shapiro-wilks tes) , (Bartletts test) 46 16, 36 . I ISO 16712： 2006 8 t (Students t-test)37 . , . , (Wilcoxon Rank Sum test)46 . (Bartletts test) F (Hartleys F tes) . , , (Dunnetts test), (Williams test)50, 51 (ANOVA) 3, 18, 21, 37, 46. ISO/TS 2028155 23 . 8.2 . , , 9, 25, 26, 30, 43, B 2
7、1, 46. . . , 21, 43. 9. 17, 20. ( ), ( ), ( ) 3, 18, 20, 33, 41, 46. , 14, 28, 39, 42. ( ) (pH 8.00.5 ) 3 LC50 33. 4 LC50 , 6.3 . 3 ( 1 ) . 2 10 ( 20 ) . , 10 LC50 20. 10 1 . 7, 20. , 90 % . , , pH, AV S ( ) . I ISO 16712： 2006 9 . . 1： 4 ( ) . 4 . , . / , 4 20. . 6. . . 2 17, 20. , , . 10. C( ) . 1
8、1. . a) b) c) ( ) d) ( ) e) ( , , AVS/SEM, , , (BOD), (SOD), pH, , ) f) , , , g) , h) i) , , j) ( , , , ) k) l) m) ( , , ) n) o) p) ( , , pH, , TOC/DOC, , , ) q) r) ; , LC50 s) t) I ISO 16712： 2006 10 A() ASTM E729： 1996 . A.1 ( ) 890 mL . . A.1 NaF 3 mgSrCl2 6H2O 20 mg H3BO330 mg KBr 100 mg KCl 700
9、 mg CaCl2 2H2O 1.47 g Na2SO44.00 MgCl2 6H2O 10.78 mg NaCl 23.50 mg Na2SiO3 9H2O 20 mg Na4EDTA(1) 1 mg NaHCO3200 mg (1) , . , , , , EDTA , 15. 1 L (340.5) g/kg pH (8.00.2) . , . I ISO 16712： 2006 11 B() , B.1 . B.1 mm g/kg Amphiporeia virginiana 5 1535 (102) Corophium volutator 12 1030 (152) Gammurus
10、 locusta 5 2035 (152) Ampelisca abdita 8 1030 (202) Leptocheirus plumulosus 5 1.532 (252) Corophium arenarium 10 2634 (152) Monoporeia affinis 220 (42) Eohaustorius estuarius 5 434 (152) Eohaustorius washingtonianus 5 1535 (152) Foxiphalus xiximeus 6 2535 (152) Rhepoxynius abronius 5 2535 (152) Gran
11、diderella japonica 6 3035 (176) Ampelisca abdita 8 1035 (202) Corophium orientale 4 0 36 (152) 3, 13, 18, 21, 24, 27, 32, 33, 38, 46 . (4.1.2 ). I ISO 16712： 2006 12 C() BEQUACM , 1416 Corophium volutator 10 Bioban(1) lvermectin(1) 2 . C.129 (1)Bioban lvermectin . , ISO . C.1 Corophium volututor % %
12、 BiobanTMbactericide 12 7.9 24.2 IvermectinTM-Round 1 14 6.7 28.0 IvermectinTM-Round 2 14 8.8 20.2 10 1995 10 . Black Rock Harbour . Ampelisca abdita, Eohaustorius estuarius Leptocheirus plumulosus 3 . 7 % Black Rock Harbour C.234 . C.2 3 % % Ampelisca abdita 6 70.7 27.0 38.9 Eohaustorius estuarius
13、8 68.1 14.7 21.6 Leptocheirus plumulo 7 46.3 27.7 59.8 1991 Eohaustorius estuarius, Amphiporeia virginiana, Corophium volutator, Eohaustorius washingtonianus, Foxiphalus xiximeus Rhepoxynius abronius 6 3 . 28. I ISO 16712： 2006 13 I ISO 16712： 2006 14 I ISO 16712： 2006 15 I ISO 16712： 2006 16 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ KSKSKSSKSKS KSKS SKS KSKS SKSKS KSKSKSKorean Agency for Tec
