1、 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS 2006 10 27 http:/www.kats.go.krKS I ISO 23753 2 2 ： Iodoterazolium chloride(INT) KS I ISO 23753 2： 2006 (2011 ) I ISO 23753 2： 2006 : ( ) ( ) KIST ( ) : (http:/www.standard.go.kr) ： ：2006 10 27 ：2011 12 26 2011-0622 ： ： ( 02- 509-7270) (http:/www.kats.go.kr
2、). 10 5 , . ICS 13.080.30 KS I ISO 2 ： Iodoterazolium chloride(INT) 23753 2： 2006(2011 ) Soil quality Determination of dehydrogenase activity in soils Part 2： Method using iodotetrazolium chloride(INT) 2005 1 ISO 23753 2 Soil quality Determination of dehydrogenase activity in soils Part 2： Method us
3、ing iodotetrazolium chloride(INT) . 1. 2 (4 iodophenyl) 3 (4 nitrophenyl) 5 phenyltetrazolium chloride(INT) . INT O2 , KS M ISO 23753 1 TTC . ( ) (L, F, H ) ( ) . 2. . . KS M ISO 10381 6 ( , ) KS M ISO 10390 pH KS M ISO 11259 KS M ISO 11465 ( ) 3. INT 30 18 . iodonitrotetrazolium formazan(INTF) ( )
4、485 nm . 1. 1 . 2. , . ( ) 3. (II) INT . 4. 4.1 KS M ISO 10381 6 . 4 3 . KS M ISO 11465 I ISO 23753 2： 2006 2 . , . 4.2 c(HCl) 1 mol/L 4.3 (Tris) c 0.1 mol/L tris(hydroxymethyl)aminomethane 12.11 g 600 mL pH . a) 2 % 7.7 b) 2 % 7.9 1 mol/L pH 1 000 mL . pH KS M ISO 10390 . 4.4 (INT) 2 (4 iodophenyl)
5、 3 (4 nitrophenyl) 5 phenyltetrazolium chloride 900 mg 100 mL (4.3) 100 mL . 1 2 . INT . . KS M ISO 11259 . pH 7.7 INT 5 mg/mL . INT 7 mg/mL . 9 mg/mL . 4.5 4.6 INTF 4.6.1 INTF 100 mL iodonitrotetrazolium formazan 50 mg (4.5) 100 mL . 4.6.2 INTF 50 mL 0 mL, 0.5 mL, 1.0 mL, 2.0 mL, 3.0 mL, 4.0 mL ( .
6、) 10 mL . mL INTF 0 g, 5 g, 10 g, 20 g, 30 g, 40 g . INT INTF . 5. 5.1 5.2 pH 5.3 2 cm 30 mL ( 5 g, 6. ). 5.4 (301) . 5.5 , , , 5.6 (90 100 ). 5.7 5.8 6. 4 (5.3) 2.00 5.00 g . 3 (4.4) 2 5 mL . (4.4) (4.3) 2 5 mL . 30 18 I ISO 23753 2： 2006 3 . triphenylformazan , (4.5) 20 mL 2 . , 1 2 . . 1 485 nm .
7、 7. ( ) . tmVa=DM100)(bscs a： (g/g) cs ： INTF (g/mL) bs ： INTF (g/mL) V： (5 mL) (25 mL) (mL) m： (g) DM： KS M ISO 11465 (%) t： ( 18 )(h) 8. . a) b) (4.1) c) d) ( 7 ) e) f) I ISO 23753 2： 2006 4 1 SPOTHELFER MAGANA, J., THALMANN, A. and SCHWEIKLE, V.： Methode zur Bestimmung der Dehydroge naseaktivitt
8、von Bden unter Einsatz von Iodotetrazoliumchlorid(INT)： Chemische Reduktion in autoklavierten sowie bestrahlten Bden sowie der Einflu der Inkubationstemperatur und zeit. Agribiol. Res., 46, 1993, pp. 250 268 2 THALMANN, H.： Zur Methodik der Bestimmung der Dehydrogenaseaktivitt im Boden mittels Triph
9、enyltetrazoliu mchlorid(TTC). Landwirtsch. Forsch., 21, 1968, pp. 243 258 3 GLATHE, H. and THALMANN, H.： Microbial activity and its relationship to fertility characteristics of some agricultural soils, with particular reference to dehydrogenase activity. Part 2. Zbl Bakt II, 124, 1970, pp. 24 36 4 M
10、ERSI, W. VON and SCHINNER, F.： An improved and accurate method for determining the activity of soils with iodotetrazolium chloride. Bio. Fertil. Soils, 11, 1991, pp. 216 220 5 HLINGER, R.： Determination of dehydrogenase activity using TTC. In： Methods in soil biology. (SCHINNER, F., HLINGER, R., KAN
11、DELER, E. and MARGESIN, R. eds.). Springer Verlag, Berlin/Heidelberg/New York, 2nd edn., 1993 6 FUCHS, M., KOCH, C. and WILKE, B. M.： Modification of the determination of dehydrogenase activity using tetrazolium chloride for soils contaminated by heavy metals. VDLUFA Schriftenreihe, 38, 1994, pp. 89
12、9 902 7 KS M ISO 10693 ( ) 2 ： Iodoterazolium chloride(INT) 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ KSKSKSSKSKS KSKS SKS KSKS SKSKS KSKSKSKorean Agency for Technology and Standards http:/www.kats.go.kr KS I ISO 23753 2： 2006 Soil qualityDetermination of dehydrogenase activity in soilsPart 2： Method using iodotetrazolium chloride(INT) ICS 13.080.30
