1、 Collection of SANS standards in electronic format (PDF) 1. Copyright This standard is available to staff members of companies that have subscribed to the complete collection of SANS standards in accordance with a formal copyright agreement. This document may reside on a CENTRAL FILE SERVER or INTRA
2、NET SYSTEM only. Unless specific permission has been granted, this document MAY NOT be sent or given to staff members from other companies or organizations. Doing so would constitute a VIOLATION of SABS copyright rules. 2. Indemnity The South African Bureau of Standards accepts no liability for any
3、damage whatsoever than may result from the use of this material or the information contain therein, irrespective of the cause and quantum thereof. ISBN 978-0-626-22319-9 SANS 5811:2009Edition 2SOUTH AFRICAN NATIONAL STANDARD Pesticides Rearing and handling of the lesser grain borer (Rhizopertha domi
4、nica F.) Published by SABS Standards Division 1 Dr Lategan Road Groenkloof Private Bag X191 Pretoria 0001Tel: +27 12 428 7911 Fax: +27 12 344 1568 www.sabs.co.za SABS SANS 5811:2009 Edition 2 Table of changes Change No. Date Scope Foreword This South African standard was approved by National Committ
5、ee SABS SC 1028F, Pesticides Laboratory facilities and rearing and handling of test animals, in accordance with procedures of the SABS Standards Division, in compliance with annex 3 of the WTO/TBT agreement. This document was published in February 2009. This document supersedes SABS SM 811:1978 (fir
6、st edition). SANS 5811:2009 Edition 2 1 Pesticides Rearing and handling of the lesser grain borer (Rhizopertha dominica F.) 1 Scope This standard specifies a method for the rearing and handling of the lesser grain borer (Rhizopertha dominica F.). 2 Normative references The following referenced docum
7、ents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. Information on currently valid national and international standards can be obta
8、ined from the SABS Standards Division. SANS 5808, Pesticides General conditions and equipment for the rearing and handling of test insects. SANS 3310-1/ISO 3310-1, Test sieves Technical requirements and testing Part 1: Test sieves of metal wire cloth. 3 Rearing conditions Maintain the rearing condit
9、ions prescribed in SANS 5808. 4 Rearing equipment 4.1 Rearing containers and lids, type A rearing containers and type A rearing container lids as described in SANS 5808, or any other suitable round, square or rectangular containers. 4.2 Sieves, two type A sieves as described SANS 5808 of nominal ape
10、rture sizes 850 m and 500 m, with the former placed on top of the latter, and that comply with the relevant requirements of SANS 3310-1. 5 Rearing medium Unground barley, heat-sterilized at 60 C 5 C for 30 min. NOTE Crushed maize or wheat may be used as an alternative. 6 Procedure 6.1 Place 10 mL of
11、 adult lesser grain borers on 1 kg of the rearing medium (see clause 5) in a rearing container (see 4.1). SANS 5811:2009 Edition 2 2 6.2 After 2 weeks, transfer the entire contents of the rearing container on to the sieves (see 4.2) and sift gently. The barley will be retained on the 850 m sieve, an
12、d the adult insects on the 500 m sieve. Retain the adults for use as laid down in 6.4. 6.3 Return the barley (containing the eggs) to the rearing container. The adults will emerge after approximately four weeks. 6.4 Rear new colonies by repeating the procedure in 6.1 to 6.3 (inclusive), but using fo
13、r each colony 5 mL of the adult grain borers retained in 6.2 and 5 mL of freshly-emerged adults (see 6.3). SABS SABS Standards Division The objective of the SABS Standards Division is to develop, promote and maintain South African National Standards. This objective is incorporated in the Standards A
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