1、BRITISH STANDARD BS ISO 8899:2003 Oil of lemon petitgrain Citrus limon (L.) Burm. f. ICS 71.100.60 BS ISO 8899:2003 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 11 December 2003 BSI 11 December 2003 ISBN 0 580 43072 3 National foreword Thi
2、s British Standard reproduces verbatim ISO 8899:2003 and implements it as the UK national standard. The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oils, which has the responsibility to: A list of organizations represented on this committee can be obtain
3、ed on request to its secretary. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Ele
4、ctronic Catalogue or of British Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. aid enquirers to
5、 understand the text; present to the responsible international/European committee any enquiries on the interpretation, or proposals for change, and keep the UK interests informed; monitor related international and European developments and promulgate them in the UK. Summary of pages This document co
6、mprises a front cover, an inside front cover, the ISO title page, pages ii and iii, a blank page, pages 1 to 8, an inside back cover and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. Date Co
7、mments Reference number ISO 8899:2003(E) OSI 3002INTERNATIONAL STANDARD ISO 8899 Second edition 2003-12-01 Oil of lemon petitgrain Citrus limon (L.) Burm. f. Huile essentielle de petitgrain citronnier Citrus limon (L.) Burm. f. BSISO8899:2003IS:9988 O3002(E) DPlcsid Fremia ihTs PDF file mya cnotiae
8、nmdebt dedyfepcaes. In ccacnadroe witA hebods licnesilop gnic,y tihs file mirp eb yantiv ro deweb detu slahl ont ide ebtlnu desse tt ehypfecaes wihce era hml era deddebicsnede ti dna onstallno de tc ehomuptfrep reromign tide ehtin.g In wodlnidaot gnihs fil,e trapies tpecca tiereht ner ehssnopiiblito
9、 yf nto ifnirigngn Aebodl sicsneilop gnic.y ehT IStneC Olar Secrteiraat caceptl on siibality in this .aera Ai ebods a tedarmfo kra Aebod SystemI snctaropro.de teDials fo teh sfotwcudorp erats sut deo crtaee tihs PDF file cna f ebi dnuon tlareneG eh Ifno lertait evo tf ehil;e teh PDc-Frtaeino marapte
10、res wtpo ereimizf deirp rotni.gn Evc yresah era neeb tkane tsne ot erutah teh fili es siutlbae fosu rI yb eSO memdob rebeis. nI tlnu ehikletneve y ttah lborp aem lertait gno it is f,dnuo plsaee ifnrom ttneC ehlar Secterirata ta teh serddaig sleb nevwo. ISO 3002 All irthgs erse.devr lnUeto sswrehise
11、specified, on trap fo this lbupictaion maeb y cudorperro de tuilizi den yna form ro na ybm ynae,s lecetrinoc ro mceinahcla, incliduntohp gcoiypodna gn micrfoilm, wittuoh repmissii now nritign from ietI rehSa Ot tsserdda eh ebolw or ISOs memreb i ydobn the cnuotfo yr ttseuqer ehe.r ISO cirypothg fofi
12、ce saCe tsopale 65 eneG 1121-HC 02 av leT. 4 + 10 947 22 1 11 xaF0 947 22 14 + 9 74 E-mial coirypthgis.o gro We bwww.is.o groii ISO 3002 Allr ihgtsser edevrBSISO8899:2003IS:9988 O3002(E) I SO 3002 All irhgts seredevr iiiForeword ISO (the International Organization for Standardization) is a worldwide
13、 federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that
14、committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. International Standards are drafted in acc
15、ordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an International Standard requ
16、ires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO 8899 was prepared by Technica
17、l Committee ISO/TC 54, Essential oils. This second edition cancels and replaces the first edition (ISO 8899:1991), which has been technically revised. Together with the second edition of ISO 855 and the third edition of ISO 3519, it also will cancel and replace ISO 7611:1985. BSISO8899:2003blank BSI
18、SO8899:2003INTENRATIONAL TSANDADR IS:9988 O3002(E)I SO 3002 All irhgts seredevr 1Oil of lemon petitgrain Citrus limon (L.) Burm. f. 1 Scope This International Standard specifies certain charac- teristics of the oil of lemon petitgrain Citrus limon (L.) Burm. f., in order to facilitate assessment of
19、its quality. 2 Normative references The following referenced documents are indispen- sable for the application of this document. For dated references, only the edition cited applies. For un- dated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 21
20、0, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General rules for la- belling and marking of containers ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Deter
21、mination of refrac- tive index ISO 592, Essential oils Determination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 1242, Essential oils Determination of acid value ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of c
22、hromatographic profiles for presentation in stan- dards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions
23、 apply. 3.1 oil of lemon petitgrain essential oil obtained by steam distillation of the leaves and twigs, usually without small green fruits, of Citrus limon (L.) Burm. f., of the Rutaceae family, growing in Spain and Italy NOTE For information on the CAS number, see ISO/TR 21092. 4 Requirements 4.1
24、 Appearance Clear liquid. 4.2 Colour Pale yellow to amber yellow. 4.3 Odour Ethereal, lemon smell. 4.4 Relative density at 20 C, 20 20 d Minimum: 0,860 Maximum: 0,887 4.5 Refractive index at 20 C Minimum: 1,472 Maximum: 1,479 4.6 Optical rotation at 20 C Between +14 and +35 BSISO8899:2003IS:9988 O30
25、02(E) 2 I SO 3002 All irhgts seredevr4.7 Miscibility in ethanol, 85 % (volume fraction), at 20 C It shall not be necessary to use more than 6 to 8 volumes of ethanol, 85 % (volume fraction), to ob- tain a clear solution with 1 volume of essential oil. 4.8 Acid value Maximum: 2,0 4.9 Chromatographic
26、profile Analysis of the essential oil shall be carried out by gas chromatography. In the chromatogram obtained, the representative and characteristic components shown in Table 1 shall be identified. The proportions of these components, indicated by the integrator, shall be as shown in Table 1. This
27、constitutes the chromatographic profile of the essential oil. Table 1 Chromatographic profile Component Minimum % Maximum % -Pinene 1,0 2,0 Sabinene 2,0 4,5 -Pinene 12,0 21,0 Limonene 30,0 42,0 trans-Ocimene 1,5 3,0 Linalol 0,2 3,5 Neral 3,0 11,0 Geranial 4,0 15,0 Neryl acetate 1,5 7,0 Geranyl aceta
28、te 1,0 4,0 NOTE The chromatographic profile is normative, contrary to typical chromatograms given for information in Annex A. 4.10 Flashpoint Information on the flashpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 25 ml. NOTE This volume allows each of the tests spe
29、cified in this International Standard to be carried out at least once. 6 Test methods 6.1 Relative density at 20 C, 20 20 d See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Miscibility in ethanol, 85 % (volume fraction), at 20 C See ISO 875. 6.5 Ac
30、id value See ISO 1242. 6.6 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BSISO8899:2003IS:9988 O3002(E) I SO 3002 All irhgts seredevr 3Annex A (informative) Typical chromatograms of the analysis by gas chromatograp
31、hy of the essential oil of lemon petitgrain Citrus limon (L.) Burm. f. BSISO8899:2003IS:9988 O3002(E) 4 I SO 3002 All irhgts seredevrBSISO8899:2003IS:9988 O3002(E) I SO 3002 All irhgts seredevr 5Peak identification 1 -Thujene 2 -Pinene 3 Camphene 4 6-Methyl-5-hepten-2-one 5 Sabinene 6 -Pinene 7 Myrc
32、ene 8 -Phellandrene 9 -3-Carene 10 -Terpinene 11 p-Cymene 12 Limonene 13 cis-Ocimene 14 trans-Ocimene 15 -Terpinene 16 Terpinolene 17 Linalol 18 Citronellal 19 Terpinen-4-ol 20 -Terpineol 21 Nerol 22 Neral 23 Geraniol 24 Geranial 25 Citronellyl acetate 26 Neryl acetate 27 Geranyl acetate 28 -Caryoph
33、yllene Operating conditions Column: fused silica capillary; length 60 m; internal diameter 0,25 mm Stationary phase: methyl silicone (DB-1 ) Film thickness: 0,25 m Oven temperature: temperature programming from 60 C to 75 C at a rate of 1 C/min, then 2 C/min to 110 C and 3 C/min to 250 C Injector te
34、mperature: 260 C Detector temperature: 270 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,05 l Carrier gas flow rate: 1 ml/min Split ratio: 1/80 Figure A.1 Typical chromatogram taken on an apolar column BSISO8899:2003IS:9988 O3002(E) 6 I SO 3002 All irhgts seredevrPeak iden
35、tification 1 -Pinene + -thujene 2 Camphene 3 -Pinene 4 Sabinene 5 -3-Carene 6 Myrcene 7 Limonene 8 cis-Ocimene 9 6-Methyl-5-hepten-2-one 10 Linalol 11 -Caryophyllene + terpinen-4-ol 12 Neral 13 -Terpineol 14 Neryl acetate 15 Geranial 16 Geranyl acetate Operating conditions Column: capillary; length
36、60 m; internal diameter 0,25 mm Stationary phase: poly(ethylene glycol) 10 000 (Supelcowax 10 ) Film thickness: 0,25 m Oven temperature: isothermal at 70 C for 8 min, then temperature programming from 70 C to 200 C at a rate of 4 C/min Injector temperature: 260 C Detector temperature: 270 C Detector
37、: flame ionization type Carrier gas: nitrogen Volume injected: 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/50 Figure A.2 Typical chromatogram taken on a polar column BSISO8899:2003IS:9988 O3002(E) I SO 3002 All irhgts seredevr 7Annex B (informative) Flashpoint B.1 General information For sa
38、fety reasons, transport companies, insurance companies, and people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was diffi
39、cult to recommend a single apparatus for stan- dardization purposes, given that: there is a wide variation in the chemical compo- sition of essential oils; the volume of the sample needed in certain re- quirements would be too costly for high-priced essential oils; as there are several different typ
40、es of equip- ment which can be used for the determination, users cannot be expected to use one specified type only. Consequently, it was decided to give a mean value for the flashpoint in an informative annex to each International Standard, for information, in order to meet the requirements of the i
41、nterested parties. The equipment with which this value was obtained should be specified. For further information see ISO/TR 11018. B.2 Flashpoint of the essential oil of lemon petitgrain The mean value is + 67 C. NOTE Obtained with “Pensky-Martens“ equipment. BSISO8899:2003IS:9988 O3002(E) 8 I SO 30
42、02 All irhgts seredevrBibliography 1 ISO 855:2003, Oil of lemon Citrus limon (L.) Burm. f., obtained by expression 2 ISO 3519:1997, Oil of lime Citrus aurantifolia (Christm.) Swingle, obtained by distillation 3 ISO/TR 11018:1997, Essential oils General guidance on the determination of flashpoint 4 I
43、SO/TR 21092: 1) , Essential oils Characterization 1) To be published. BSISO8899:2003blankBS ISO 8899:2003 BSI 389 Chiswick High Road London W4 4AL BSI British Standards Institution BSI is the independent national body responsible for preparing British Standards. It presents the UK view on standards
44、in Europe and at the international level. It is incorporated by Royal Charter. Revisions British Standards are updated by amendment or revision. Users of British Standards should make sure that they possess the latest amendments or editions. It is the constant aim of BSI to improve the quality of ou
45、r products and services. We would be grateful if anyone finding an inaccuracy or ambiguity while using this British Standard would inform the Secretary of the technical committee responsible, the identity of which can be found on the inside front cover. Tel: +44 (0)20 8996 9000. Fax: +44 (0)20 8996
46、7400. BSI offers members an individual updating service called PLUS which ensures that subscribers automatically receive the latest editions of standards. Buying standards Orders for all BSI, international and foreign standards publications should be addressed to Customer Services. Tel: +44 (0)20 89
47、96 9001. Fax: +44 (0)20 8996 7001. Email: ordersbsi-. Standards are also available from the BSI website at http:/www.bsi-. In response to orders for international standards, it is BSI policy to supply the BSI implementation of those that have been published as British Standards, unless otherwise req
48、uested. Information on standards BSI provides a wide range of information on national, European and international standards through its Library and its Technical Help to Exporters Service. Various BSI electronic information services are also available which give details on all its products and services. Contact the Information Centre. Tel: +44 (0)20 8996 7111. Fax: +44 (0)20 8996 7048. Email: infobsi-. Subscribing members of BSI are kept up to date with standards developments and receive substantial discounts on the purchase price of standards. For details of these and other benefits con
