1、Designation: D1292 15Standard Test Method forOdor in Water1This standard is issued under the fixed designation D1292; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year of last revision. A number in parentheses indicates the
2、year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 This test method2covers the determination of the odor(that is, the property that affects the sense of smell) of water.Asuggested system for classifying odors is given as A
3、nnex A1.The test method is applicable to the determination of odorintensity in terms of odor intensity index or threshold odornumber.1.2 Effluents may carry a myriad of compounds, difficult tomeasure individually, which contribute to odor problems.Combinations of compounds can cause an odor intensit
4、y ordevelop a characteristic that cannot be anticipated from odorsof the individual substances.1.3 Because of the variation in human sensitivity, highprecision in determining odor intensity is not possible. Therewill not always be agreement on odor characteristics by varioustesters. Odor analysis pr
5、ovides the tool to measure variation inodor intensity at a given sampling point. The degree ofvariation may indicate the magnitude or importance of an odorproblem. Determining the cause of the variation or the sourceof the objectionable characteristic may define the odor problembetter than analysis
6、for individual compounds.1.4 The values stated in SI or inch-pound units are to beregarded as the standard. The values given in parentheses arefor information only.1.5 This standard does not purport to address all of thesafety concerns, if any, associated with its use. It is theresponsibility of the
7、 user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitations prior to use. For specific hazardstatements, see the warning statement under 10.1.2. Referenced Documents2.1 ASTM Standards:3D1066 Practice for Sampling SteamD1129
8、Terminology Relating to WaterD1193 Specification for Reagent WaterD2777 Practice for Determination of Precision and Bias ofApplicable Test Methods of Committee D19 on WaterD3370 Practices for Sampling Water from Closed Conduits3. Terminology3.1 Definitions:3.1.1 The terms odor-intensity index and od
9、or thresholdnumber in this standard are defined in accordance withTerminology D1129 as follows:3.1.2 odor-intensity index, nthe number of times theconcentration of the original sample is halved by addition ofodor-free water to obtain the least definitely perceptible odor.3.1.3 odor threshold number,
10、 nthe greatest dilution of thesample with odor-free water to yield the least definitelyperceptible odor.3.2 For definitions of other terms used in this test method,refer to Terminology D1129.4. Summary of Test Method4.1 A sample of water is diluted with odor-free water untila dilution is obtained th
11、at has the least definitely perceptibleodor. The test is made by two or more testers. One makesdilutions and the others determine odor intensity. Samples aretested in generally increasing concentration of odorant, al-though not in consecutive sequence of dilutions, until the odoris perceived. The pe
12、rsons making the test select the odoroussample from among three flasks, two of which contain odor-free water. Odor is measured without regard to the presence ofsuspended matter or immiscible substances in the sample.1This test method is under the jurisdiction of ASTM Committee D19 on Waterand is the
13、 direct responsibility of Subcommittee D19.05 on Inorganic Constituentsin Water.Current edition approved Oct. 1, 2015. Published October 2015. Originallyapproved in 1953. Last previous edition approved in 2010 as D1292 10. DOI:10.1520/D1292-15.2This test method is based on a procedure proposed by th
14、e Dow Chemical Co.in a private communication.3For referenced ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.Copyright ASTM I
15、nternational, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States1Cognizance is taken of the fact that there is no absolute odorvalue and that the test is to be used for comparison only. Thetest is carried out at 40C.5. Significance and Use5.1 The odor of water is a s
16、ubjective property which isrecognized as having a significant effect on its quality. This testis intended to provide a reproducible test method for determin-ing the intensity of odor in waters for comparative or controlpurposes.5.2 The test may be useful in checking the quality of raw ortreated wate
17、rs, determining the effectiveness of treatmentprocedures, or in tracing sources of contamination or leaks inindustrial processes.5.3 The results of the test are very dependent upon theobservers, since the sensitivity of individuals to odor is highlyvariable and changes from day to day. Careful stand
18、ardizationof the conditions is essential.6. Interferences and Precautions6.1 The area used for the test shall be free of interferingodors.4An ideal laboratory has a separate room equipped withactivated-carbon filtered inlet air of controlled, constant tem-perature and humidity. A relative humidity o
19、f 50 % is recom-mended wherever control is feasible. Cleanliness is an absolutenecessity.All equipment used in the test shall be clean and freeof odor and shall be restricted to use for odor determination.Anodorless detergent shall be used to cleanse the hands and facesof the persons participating i
20、n the test from tobacco, shavingpreparation, cosmetic, and other odors. Testers shall not smoke,chew tobacco or gum, or eat food of pronounced taste or odorfor at least 30 min prior to the determination.6.2 The physical condition of the participants is important.The odor tester shall be free from an
21、y conditions affecting theolfactory system. Prolonged use of the sense of smell causesolfactory fatigue. Repeated smelling of the same odor has thesame effect. Therefore, for prolonged testing, frequent restperiods, preferably in fresh, odor-free air, are necessary forrecuperation. Under ordinary ci
22、rcumstances no operator shallcarry out odor tests for longer than 15 min without rest in orderto avoid olfactory fatigue. This is an average time. Strongerodorants may dull olfactory response within a few minuteswhile waters of good quality may be tested over longer timeintervals. If personnel are l
23、imited, the testers may check theirobservations after allowing sufficient time to relax the olfactorysystem.6.3 Not all persons are capable of carrying out this test. Thetesters should be thoroughly screened to obtain the bestpossible precision, especially for research purposes. However,if due care
24、is exercised, most persons qualify for routine work.At least two testers are necessary, but more are preferred: oneto make the preliminary screening and prepare the dilutionsand the other or others to make the actual odor determination.The testers making the determination shall not know thedilutions
25、; in no case shall they make the dilutions. Dilutionsshall be tested by presenting samples ranging from lower tohigher concentrations but they must not be presented insequence. Insertion of a set of blanks or lower concentrationsinto the series is recommended. This lessens the chance ofmemorizing od
26、ors or guessing.6.4 Color is often imparted by various contaminants inwastewater. This color is often evident below perceptible odorlevels. A colored lighting system may be used to eliminatecolor bias in selection of the odor-containing flask by thetesters. Photographic safelights with interchangeab
27、le filters areuseful for this purpose.6.5 Turbidity in some wastewaters may be evident belowperceptible odor levels. The colored lighting system describedin 6.4 may not eliminate this bias. In such instances, externalmasking of the flasks may be necessary. Painting the flasks tomake them opaque is a
28、 means of masking turbidity.6.6 For maximum control, the odor laboratory5should bedivided into two areas separating the sample preparation andthe odor detection activities. This allows isolation of thedilution operator from the odor tester and permits greatercontrol of background odor in the odor me
29、asuring area.7. Apparatus7.1 Constant-Temperature Bath, capable of maintaining atemperature of 40 6 1C.7.2 Sample Bottles, Glass-StopperedBiochemical oxygendemand (BOD) bottles are satisfactory for this purpose.7.3 Flasks, 500 mL, wide-mouth Erlenmeyer, glass-stoppered or covered by watch glasses.8.
30、 Reagents8.1 Activated Carbon, water purification grade. Carbonshould be renewed after treating approximately 20 L of water,or more often as necessary.8.2 Water, Odor-FreePrepare odor-free water by passingreagent water conforming to Specification D1193, Type I or II,through a glass column 0.9 m (3 f
31、t) long and 51 mm (2 in.) indiameter, packed with granular activated carbon, at a flow rateof less than 11 L/h. The water used to prepare odor-freedilution water shall have a total dissolved solids content notexceeding that of the sample being tested. Use glass connec-tions and tubing in making the
32、system. The column ends maybe packed with glass wool to support the carbon. Test thecolumn effluent at 40C. This is necessary since the quantitiesand nature of impurities in the water will affect useful carbonlife. It has been found that columns used infrequently maydevelop a biological growth which
33、 imparts odor. To check thecondition of the column after an idle period (such as aweekend) a simple test is recommended. Fill a short glass tubewith fresh carbon and filter water through it. The reagent waterso prepared should be checked against the column effluent tobe sure a subtle odor is not pre
34、sent. Odor-free water should not4Baker, R. A., “Critical Evaluation of Olfactory Measurement,” Journal of theWater Pollution Control Federation, Vol 34, No. 6, June 1962, pp. 582591.5Baker, R. A., “Odor Testing Laboratory,” Journal of the Water PollutionControl Federation, Vol 35, No. 11, November 1
35、963, pp. 13961402.D1292 152be stored but should be prepared on the day the test is made. Inorder to save time during analyses, maintain the supply ofodor-free water at 40 6 1C.9. Sampling9.1 Collect the sample in accordance with the applicablepractice, Practices D1066 and D3370.9.2 Determine odor on
36、 separate, freshly-obtained samples.Sampling is very important. Glass-stoppered bottles shall beused and shall be completely filled. Although larger volumesmay be necessary in some cases, the standard BOD bottles areideal for this use. If the sample is at a temperature greater than40C, cool it befor
37、e testing for odor.9.3 Storage of water may lead to errors through modifica-tion of odor intensity and character. Biological, chemical, andphysical reactions are factors in this degradation. If the analysiscannot be made promptly, refrigerate the sample during stor-age.Although this will not guarant
38、ee that odor changes will nottake place, it minimizes the effect in most cases. Store thesample in a glass-stoppered bottle to minimize contaminationwith refrigerator odors. Precooling the sample in an ice bathand in an odor-free atmosphere before refrigeration is advised.9.4 Record the sample tempe
39、rature at the time of collection.This frequently is useful when relating laboratory results tofield conditions.10. Preliminary Test10.1 Preparation of a test series can be greatly simplified ifan approximation of odor intensity is first made as follows:thoroughly scrub all glassware with a brush and
40、 odorlessdetergent. Rinse with tap water and clean with chromic acidsolution. Rinse with reagent water, rinse with odor-free water,and store filled with odor-free water. Check all flasks to makesure no residual odor exists by testing with 200 mLof odor-freewater at 40C. (WarningChromic acid is toxic
41、 and is a verypowerful oxidizing agent. Extreme caution should be exercisedin its use.)10.2 To determine the estimated order of magnitude of theodor intensity, the dilution tester shall pipet 25 mL of sampleinto a clean, glass-stoppered (or watch-glass covered) 500 mLconical flask. Dilute this to a
42、total volume of 200 mLby adding175 mLof odor-free water at 40C. For this preliminary test thedilution water may be added from a graduated cylinder. Do notallow the pipet or the sample solution to touch the neck of theflask. Stopper and warm the flask to 40C in a water bath.Avoid prolonged or direct
43、heating.10.3 Mix by vigorously swirling three or four times, removethe stopper, and place the nose at the top of the flask. Test forodor, using normal inhalation. Compare with a flask containingodor-free water. Note whether odor is detected. If odor is notdetected, prepare lower dilutions successive
44、ly in clean flasksuntil the odor is just perceptible. It usually is convenient tomake a series of dilutions at the beginning. Odor testinghowever must be from the highest dilution toward lowerdilutions.10.4 If the odor is detected in the initial dilution, dilute atleast 12.5 mL of original sample to
45、 a measured volume andrecord this primary dilution. Make subsequent lower dilutionsand record the aliquot at which odor is just perceptible.Calculate the estimated order of magnitude of the odorintensity in accordance with Section 12.11. Procedure11.1 The choice of dilutions for odor measurement dep
46、endson the order of magnitude of odor intensity determined inaccordance with Section 10. The tester who determined theodor intensity in the preliminary test shall now assume the roleof making the dilutions for the other tester or testers, but shalldo no testing himself. Primary dilutions shall conta
47、in at least12.5 mL of sample. If greater dilutions are necessary, addodor-free water to the primary dilution. Use such subsequentdilutions in the evaluation.11.2 The dilution tester shall code three clean, odor-freeflasks for the test, adding approximately half the estimatedquantity of sample (preli
48、minary test) to one of the flasks. Dilutethe contents of each flask to a total volume of 200 mL withodor-free water. Stopper each flask and adjust the temperatureto 40C in a water bath. Vigorously swirl the stoppered flasksand present them to the odor tester. In presenting the flasks tothe tester th
49、e position of the odor-containing flasks in the arrayshall be randomized. The odor tester shall swirl a flaskvigorously exercising care to avoid spilling the contents. Theflasks shall be held by the flat bottom with a finger on the coveror stopper during swirling. This minimizes imparting an odornear the opening of the flask prior to testing. Swirling distrib-utes the odorous substance uniformly in the vapor space. Thetester shall remove the stopper or watch glass cover, place hisnose at the top of the flask and test for odor using normalinhalation. If the odor teste
