1、Designation: E 1045 00 (Reapproved 2005)Standard Specification forPipet, Sahli Hemoglobin1This standard is issued under the fixed designation E 1045; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the year of last revision. A numb
2、er in parentheses indicates the year of last reapproval. Asuperscript epsilon (e) indicates an editorial change since the last revision or reapproval.1. Scope1.1 This specification covers reusable pipets calibrated“ tocontain” 20 cmm of whole blood and used for hemoglobindeterminations.2. Referenced
3、 Documents2.1 ASTM Standards:2E 920 Specification for Commercially Packaged LaboratoryApparatusE 921 Specification for Export Packaged Laboratory Appa-ratusE 1133 Practice for Performance Testing of Packaged Labo-ratory Apparatus for United States Government Procure-mentsE 1157 Specification for Sam
4、pling and Testing of ReusableLaboratory Glassware3. Materials3.1 The pipets shall be made of common spirit bore whiteback tubing or of clear glass with a white stripe applied to theouter surface of the tubing.4. Design4.1 ShapePipets shall be straight and of one-piece con-struction. Any cross sectio
5、n of a pipet taken in a planeperpendicular to the longitudinal axis should be circular.4.2 Delivery TipsDelivery tips shall be made with agradual or concave taper to a length of 10 to 25 mm. The tipend shall be ground and tapered with fine abrasive, or fire-polished. Dimensions of the delivery tip s
6、hall be as specified inFig. 1.4.3 The top of the pipet shall be ground to a taper or formedto a funnel shape according to the dimensions specified in Fig.1.5. Markings5.1 Graduation LineThe pipet shall have one graduationline located 20 cmm from the pipet tip. The graduation lineshall be located on
7、the clear portion of the tubing and shallextend at least two thirds around the pipet and not exceed 0.4mm in width.5.2 Volumetric DesignationThe pipet shall be marked20CMM on the clear portion of the tubing with the markingslocated approximately 5 mm above the graduation line.5.3 IdentificationEach
8、pipet shall be marked with themanufacturers name or trademark on the white stripe portionof the pipet. Catalog number markings are optional. Allmarkings shall be permanently fused onto the pipet. Themarkings shall be amber or black in color. When tested inaccordance to 6.3, the pigmentation shall no
9、t discolor. Theappearance of the markings, when viewed by the eye undernormal room lighting, shall be the same before and aftertesting.5.4 Capacity DeviationSahli Hemoglobin pipets are madewith maximum capacity deviation of 6 1.0% or6 2.0 %. Theselected capacity deviation shall be marked on the clea
10、r orwhite stripe portion of the pipet. The capacity of the pipet shallbe within the selected capacity deviation marked on the pipetwhen tested as specified in 6.2.6. Testing6.1 Capacity TestThe capacity of the pipet shall bedetermined by means of using distilled water and a weighingdevice with weigh
11、t sensitivity not less than 0.001 mg.6.1.1 The pipet shall be thoroughly cleaned, dried, andallowed to adjust to room temperature.6.1.2 The pipet shall be weighed and the weight recorded.6.1.3 The pipet shall be filled to the calibration line withdistilled water and weighed, and the weight recorded.
12、6.1.4 The recorded weight of the clean and dry pipet shall besubtracted from the recorded weight of the distilled water-filledpipet providing the observed volumetric capacity (Vc)ofthepipet in grams.6.1.5 The observed volumetric capacity ( Vt) shall then becorrected to actual volumetric capacity at
13、20C (Vc), deter-mined by:1This specification is under the jurisdiction of ASTM Committee E41 onLaboratory Apparatus and is the direct responsibility of Subcommittee E41.01 onApparatus.Current edition approved Oct. 1, 2005. Published December 2005. Originallyapproved in 1985. Last previous edition ap
14、proved in 2000 as E 1045 00.2For referenced ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM website.1Copyright ASTM International, 10
15、0 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.Vc5Vt1 1 a t 2 20C!(1)where:Vt= observed volumetric capacity at t C, grams,Vc= corrected volumetric capacity at 20C,a = coefficient of cubical expansion of pipetglass, = 0.000072/C 0.000084/C (dependent uponsource of m
16、aterial), andt = temperature recorded during weighing, C.6.2 Capacity DeviationThe capacity deviation of thepipet is the difference between the stated capacity V1and thecorrected observed capacity Vc, and is determined by thefollowing expression:Capacity deviation, % 5100 Vc2 V1!V1(2)6.3 Pigmentatio
17、n TestPrepare a fresh chromic acid clean-ing solution by combining 200 g of solution dichromate (Na2Cr2O72H2O), 1000 mLof water, and 1500 mLof sulfuric acid(H2SO4, ACS Reagent95 to 98 %). Immerse the pipets inthe chromic acid solution. Let stand at room temperature (20 to25C) for 15 min. Remove the
18、pipets from the solution andthoroughly rinse in distilled water. Dry the pipets by rubbingvigorously, 5 to 10 strokes, with a laboratory cloth or tissue.The appearance of the markings should be the same as beforethe test, when judged by the eye under normal room lighting.6.4 Strain Free TestThe pipe
19、ts shall be free from strainwhen viewed under a polariscope.7. Sampling and Testing7.1 See Specification E 1157.8. Packaging8.1 Select one from Specifications E 920, E 921 or PracticeE 1133.9. Keywords9.1 disposal; glass; tube; WestergrenFIG. 1 Pipet, Sahli HemoglobinE 1045 00 (2005)2ASTM Internatio
20、nal takes no position respecting the validity of any patent rights asserted in connection with any item mentionedin this standard. Users of this standard are expressly advised that determination of the validity of any such patent rights, and the riskof infringement of such rights, are entirely their
21、 own responsibility.This standard is subject to revision at any time by the responsible technical committee and must be reviewed every five years andif not revised, either reapproved or withdrawn. Your comments are invited either for revision of this standard or for additional standardsand should be
22、 addressed to ASTM International Headquarters. Your comments will receive careful consideration at a meeting of theresponsible technical committee, which you may attend. If you feel that your comments have not received a fair hearing you shouldmake your views known to the ASTM Committee on Standards
23、, at the address shown below.This standard is copyrighted by ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959,United States. Individual reprints (single or multiple copies) of this standard may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org).E 1045 00 (2005)3
