1、Designation: E 961 97 (Reapproved 2003)Standard Specification forBlood Sedimentation Tube, Wintrobe, Glass, Reusable1This standard is issued under the fixed designation E 961; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the yea
2、r of last revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon (e) indicates an editorial change since the last revision or reapproval.1. Scope1.1 This specification covers reusable blood sedimentationtubes suitable for determining sedimentation rates and the
3、volume of packed red blood cells.1.2 This standard does not purport to address all of thesafety concerns, if any, associated with its use. It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitat
4、ions prior to use.2. Referenced Documents2.1 ASTM Standards:E 438 Specification for Glasses in Laboratory Apparatus2E 671 Specification for Maximum Permissible Thermal Re-sidual Stress in Annealed Glass Laboratory Apparatus2E 920 Specification for Commercially Packaged LaboratoryApparatus2E 921 Spec
5、ification for Export Packaged Laboratory Appa-ratus2E 1133 Practice for Performance Testing of Packaged Labo-ratory Apparatus for United States Government Procure-ments2E 1157 Specification for Sampling and Testing of ReusableLaboratory Glassware23. Terminology3.1 Definitions of Terms Specific to Th
6、is Standard:3.1.1 reusableCapable of being used again.3.1.2 WintrobeThe surname of the individual responsiblefor the design of the Wintrobe tube and the method of use.34. Classification4.1 This specification covers a tube that is intended to beused until it is no longer considered a functional devic
7、e for thepurpose intended.5. Materials5.1 GlassThe tubes made to this specification shall befabricated from borosilicate glass, Type I, Class B, or soda-lime glass, Type II, in accordance with Specification E 438.6. Dimensions and Graduations6.1 DimensionsThe tube shall be made of tubing with anouts
8、ide diameter (O.D.) of 7.0 to 8.0 mm with an insidediameter (I.D.) of 2.9 to 3.3 mm. The uniformity of the boreshall be 60.1 mm throughout the tube. The tube shall be 110to 117 mm long and have a graduated scale of 105 6 0.25 mmfrom the inside bottom of the tube. The tube shall be legiblymarked with
9、 the manufacturers or vendors name or mark andpossess a frosted area for marking purposes.6.2 Graduation ScaleThe tube shall be graduated 105 60.25 mm in 1-mm divisions and numbered every 1 cm with twosets of numerals. One set of graduation numerals shall be from0 to 9 cm down the left side of the g
10、raduation scale and theother set of g (20 to 25C) for 15 min. Remove tube from thesolution and thoroughly rinse in tap water followed by distilledwater. Dry the tube by rubbing vigorously, 5 to 10 strokes, witha laboratory cloth or tissue. This appearance of the markingsshould be the same as before
11、the test, when judged by eyeunder normal room lighting.6.3 Resistance to Centrifugal Force TestFill the tube withwater to the top graduation line and place in a centrifuge. Thespeed and dimensions of centrifuge headshall be such that theinside bottom of the tube is subjected to arelative centrifugal
12、force of (RCF) not less than 2 500 gravities. Calculate therelative centrifugal force as follows:RCF = relative centrifugal force in gravities,RCF = 00001118 3 r 3 N2gravities,r = rotating radius to inside or outside of tube in centimetre,andN = rotating speed in revolutions per minute.6.4 For addit
13、ional sampling and testing data, see Specifica-tion E 1157.7. Packaging7.1 For packaging, select from either Specification E 920,Specification E 921, or Practice E 1133.8. Keywords8.1 blood; glass; reusable; sedimentation rate; wintrobe1This specification is under the jurisdiction of ASTM Committee
14、E41 onLaboratory Apparatus and is the direct responsibility of Subcommittee E 41.01 onGlass Apparatus.Current edition approved Nov. 10, 1997. Published February 1998. Originallypublished as E 961 83. Last previous edition E 961 93.2Annual Book of ASTM Standards, Vol 14.02.3Wintrobe, Maxwell M., “Lab
15、oratory Evaluation of Erythrocytes,” ClinicalHematology, Seventh Ed., 1974, pp. 109134.1Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.ASTM International takes no position respecting the validity of any patent rights asserted in conn
16、ection with any item mentionedin this standard. Users of this standard are expressly advised that determination of the validity of any such patent rights, and the riskof infringement of such rights, are entirely their own responsibility.This standard is subject to revision at any time by the respons
17、ible technical committee and must be reviewed every five years andif not revised, either reapproved or withdrawn. Your comments are invited either for revision of this standard or for additional standardsand should be addressed to ASTM International Headquarters. Your comments will receive careful c
18、onsideration at a meeting of theresponsible technical committee, which you may attend. If you feel that your comments have not received a fair hearing you shouldmake your views known to the ASTM Committee on Standards, at the address shown below.This standard is copyrighted by ASTM International, 10
19、0 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959,United States. Individual reprints (single or multiple copies) of this standard may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org).E 961 97 (2003)2
