1、Designation: E 961 97 (Reapproved 2008)Standard Specification forBlood Sedimentation Tube, Wintrobe, Glass, Reusable1This standard is issued under the fixed designation E 961; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revision, the yea
2、r of last revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon () indicates an editorial change since the last revision or reapproval.1. Scope1.1 This specification covers reusable blood sedimentationtubes suitable for determining sedimentation rates and thev
3、olume of packed red blood cells.1.2 This standard does not purport to address all of thesafety concerns, if any, associated with its use. It is theresponsibility of the user of this standard to establish appro-priate safety and health practices and determine the applica-bility of regulatory limitati
4、ons prior to use.2. Referenced Documents2.1 ASTM Standards:2E 438 Specification for Glasses in Laboratory ApparatusE 920 Specification for Commercially Packaged LaboratoryApparatusE 921 Specification for Export Packaged Laboratory Appa-ratusE 1133 Practice for Performance Testing of Packaged Labo-ra
5、tory Apparatus for United States Government Procure-mentsE 1157 Specification for Sampling and Testing of ReusableLaboratory Glassware3. Terminology3.1 Definitions of Terms Specific to This Standard:3.1.1 reusableCapable of being used again.3.1.2 WintrobeThe surname of the individual responsiblefor
6、the design of the Wintrobe tube and the method of use.34. Classification4.1 This specification covers a tube that is intended to beused until it is no longer considered a functional device for thepurpose intended.5. Materials5.1 GlassThe tubes made to this specification shall befabricated from boros
7、ilicate glass, Type I, Class B, or soda-lime glass, Type II, in accordance with Specification E 438.6. Dimensions and Graduations6.1 DimensionsThe tube shall be made of tubing with anoutside diameter (O.D.) of 7.0 to 8.0 mm with an insidediameter (I.D.) of 2.9 to 3.3 mm. The uniformity of the boresh
8、all be 60.1 mm throughout the tube. The tube shall be 110to 117 mm long and have a graduated scale of 105 6 0.25 mmfrom the inside bottom of the tube. The tube shall be legiblymarked with the manufacturers or vendors name or mark andpossess a frosted area for marking purposes.6.2 Graduation ScaleThe
9、 tube shall be graduated 105 60.25 mm in 1-mm divisions and numbered every 1 cm with twosets of numerals. One set of graduation numerals shall be from0 to 9 cm down the left side of the graduation scale and theother set of g (20 to 25C) for 15 min. Remove tube from thesolution and thoroughly rinse i
10、n tap water followed by distilledwater. Dry the tube by rubbing vigorously, 5 to 10 strokes, witha laboratory cloth or tissue. This appearance of the markingsshould be the same as before the test, when judged by eyeunder normal room lighting.6.3 Resistance to Centrifugal Force TestFill the tube with
11、water to the top graduation line and place in a centrifuge. Thespeed and dimensions of centrifuge headshall be such that theinside bottom of the tube is subjected to arelative centrifugalforce of (RCF) not less than 2 500 gravities. Calculate therelative centrifugal force as follows:RCF = relative c
12、entrifugal force in gravities,RCF = 00001118 3 r 3 N2gravities,r = rotating radius to inside or outside of tube in centimetre,andN = rotating speed in revolutions per minute.6.4 For additional sampling and testing data, see Specifica-tion E 1157.7. Packaging7.1 For packaging, select from either Spec
13、ification E 920,Specification E 921, or Practice E 1133.1This specification is under the jurisdiction of ASTM Committee E41 onLaboratory Apparatus and is the direct responsibility of Subcommittee E41.01 onApparatus.Current edition approved Nov. 1, 2008. Published January 2009. Originallyapproved in
14、1983. Last previous edition approved in 2003 as E 961 97 (2003).2For referenced ASTM standards, visit the ASTM website, www.astm.org, orcontact ASTM Customer Service at serviceastm.org. For Annual Book of ASTMStandards volume information, refer to the standards Document Summary page onthe ASTM websi
15、te.3Wintrobe, Maxwell M., “Laboratory Evaluation of Erythrocytes,” ClinicalHematology, Seventh Ed., 1974, pp. 109 134.1Copyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.8. Keywords8.1 blood; glass; reusable; sedimentation rate; wintrobe
16、ASTM International takes no position respecting the validity of any patent rights asserted in connection with any item mentionedin this standard. Users of this standard are expressly advised that determination of the validity of any such patent rights, and the riskof infringement of such rights, are
17、 entirely their own responsibility.This standard is subject to revision at any time by the responsible technical committee and must be reviewed every five years andif not revised, either reapproved or withdrawn. Your comments are invited either for revision of this standard or for additional standar
18、dsand should be addressed to ASTM International Headquarters. Your comments will receive careful consideration at a meeting of theresponsible technical committee, which you may attend. If you feel that your comments have not received a fair hearing you shouldmake your views known to the ASTM Committ
19、ee on Standards, at the address shown below.This standard is copyrighted by ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959,United States. Individual reprints (single or multiple copies) of this standard may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM website(www.astm.org).E 961 97 (2008)2
