1、DD CEN ISO/TS10272-3:2010ICS07.100.30NOCOPYINGWITHOUTBSIPERMISSIONEXCEPTASPERMITTEDBYCOPYRIGHTLAWDRAFT FOR DEVELOPMENTMicrobiology of foodand animal feedingstuffs Horizontalmethod for detectionand enumeration ofCampylobacter spp.Part 3: Semi-quantitative method(ISO/ TS 10272-3:2010)DD CEN ISO/TS1027
2、2-3:2010IncorporatingcorrigendumDecember 2011This Draft for Developmentwas published under theauthority of the StandardsPolicy and StrategyCommittee on 31 March2010 BSI 2010ISBN 978 0 580 61509 2Amendments/corrigenda issued since publicationDate CommentsDD CEN ISO/TS 10272-3:2010National forewordThi
3、s Draft for Development is the UK implementation of CEN ISO/TS10272-3:2010.This publication is not to be regarded as a British Standard.It is being issued in the Draft for Development series of publications andis of a provisional nature. It should be applied on this provisional basis,so that informa
4、tion and experience of its practical application can beobtained.Comments arising from the use of this Draft for Development arerequested so that UK experience can be reported to the internationalorganization responsible for its conversion to an international standard.A review of this publication wil
5、l be initiated not later than 3 years afterits publication by the international organization so that a decision can betaken on its status. Notification of the start of the review period will bemade in an announcement in the appropriate issue of Update Standards.According to the replies received by t
6、he end of the review period, the responsible BSI Committee will decide whether to support theconversion into an international Standard, to extend the life of theTechnical Specification or to withdraw it. Comments should be sent tothe Secretary of the responsible BSI Technical Committee at BritishSta
7、ndards House, 389 Chiswick High Road, London W4 4AL.The UK participation in its preparation was entrusted to TechnicalCommittee AW/9, Microbiology.A list of organizations represented on this committee can be obtained onrequest to its secretary.This publication does not purport to include all the nec
8、essary provisionsof a contract. Users are responsible for its correct application.Compliance with a British Standard cannot confer immunityfrom legal obligations.This Draft for Developmentwas published under theauthority of the StandardsPolicy and StrategyCommittee on 31 March2010 BSI 2010ISBN 978 0
9、 580 61509 2Amendments/corrigenda issued since publicationDate CommentsDD CEN ISO/TS 10272-3:2010National forewordThis Draft for Development is the UK implementation of CEN ISO/TS10272-3:2010.This publication is not to be regarded as a British Standard.It is being issued in the Draft for Development
10、 series of publications andis of a provisional nature. It should be applied on this provisional basis,so that information and experience of its practical application can beobtained.Comments arising from the use of this Draft for Development arerequested so that UK experience can be reported to the i
11、nternationalorganization responsible for its conversion to an international standard.A review of this publication will be initiated not later than 3 years afterits publication by the international organization so that a decision can betaken on its status. Notification of the start of the review peri
12、od will bemade in an announcement in the appropriate issue of Update Standards.According to the replies received by the end of the review period, the responsible BSI Committee will decide whether to support theconversion into an international Standard, to extend the life of theTechnical Specificatio
13、n or to withdraw it. Comments should be sent tothe Secretary of the responsible BSI Technical Committee at BritishStandards House, 389 Chiswick High Road, London W4 4AL.The UK participation in its preparation was entrusted to TechnicalCommittee AW/9, Microbiology.A list of organizations represented
14、on this committee can be obtained onrequest to its secretary.This publication does not purport to include all the necessary provisionsof a contract. Users are responsible for its correct application.DD CEN ISO/TS 10272-3:2010ISBN 978 0 580 77669 4Amendments/corrigenda issued since publicationDate Co
15、mments30 November 2012 Implementation of ISO corrigendum December 2011:Table 3 replacedThis Draft for Developmentwas published under theauthority of the StandardsPolicy and StrategyCommittee on 31 March 2010 The British Standards Institution 2012.Published by BSI StandardsLimited 2012National forewo
16、rdThis Draft for Development is the UK implementation of CEN ISO/TS 10272-3:2010. It is identical to ISO/TS 10272-3:2010,incorporating corrigendum December 2011.TECHNICAL SPECIFICATION SPCIFICATION TECHNIQUE TECHNISCHE SPEZIFIKATIONCEN ISO/TS 10272-3 March 2010 ICS 07.100.30English Version Microbiol
17、ogy of food and animal feeding stuffs - Horizontal method for detection and enumeration of Campylobacter spp. - Part 3: Semi-quantitative method (ISO/TS 10272-3:2010) Microbiologie des aliments - Mthode horizontale pour larecherche et le dnombrement de Campylobacter spp. - Partie 3: Mthode semi-quan
18、titative (ISO/TS 10272-3:2010)Mikrobiologie von Lebensmitteln und Futtermitteln - Horizontales Verfahren zum Nachweis und zur Zhlung von Campylobacter spp. - Teil 3: Semiquantitatives Verfahren (ISO/TS 10272-3:2010) This Technical Specification (CEN/TS) was approved by CEN on 28 December 2009 for pr
19、ovisional application.The period of validity of this CEN/TS is limited initially to three years. After two years the members of CEN will be requested to submit theircomments, particularly on the question whether the CEN/TS can be converted into a European Standard.CEN members are required to announc
20、e the existence of this CEN/TS in the same way as for an EN and to make the CEN/TS availablepromptly at national level in an appropriate form. It is permissible to keep conflicting national standards in force (in parallel to the CEN/TS) until the final decision about the possible conversion of the C
21、EN/TS into an EN is reached.CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia,Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland,Portugal, R
22、omania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom.EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATIONEUROPISCHES KOMITEE FR NORMUNGManagement Centre: Avenue Marnix 17, B-1000 Brussels 2010 CEN All rights of exploitation in any form and by any means reserved
23、worldwide for CEN national Members.Ref. No. CEN ISO/TS 10272-3:2010: EDD CEN ISO/TS 10272-3:2010CEN ISO/TS 10272-3:2010 (E)3 Foreword This document (CEN ISO/TS 10272-3:2010) has been prepared by Technical Committee ISO/TC 34 “Foodproducts“ in collaboration with Technical Committee CEN/TC 275 “Food a
24、nalysis - Horizontal methods” thesecretariat of which is held by DIN.Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. According to the
25、CEN/CENELEC Internal Regulations, the national standards organizations of the followingcountries are bound to announce this Technical Specification: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy,Latvi
26、a, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia,Spain, Sweden, Switzerland and the United Kingdom. Endorsement noticeThe text of ISO/TS 10272-3:2010 has been approved by CEN as a CEN ISO/TS 10272-3:2010 without any modification.DD CEN ISO/TS 10272-
27、3:2010CEN ISO/TS 10272-3:2010 (E)3 Foreword This document (CEN ISO/TS 10272-3:2010) has been prepared by Technical Committee ISO/TC 34 “Foodproducts“ in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” thesecretariat of which is held by DIN.Attention is drawn to
28、 the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the followingcou
29、ntries are bound to announce this Technical Specification: Austria, Belgium, Bulga ia, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, I eland, Ireland, Italy,Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia,
30、 Slovenia,Spain, Sweden, Switzerland and the United Kingdom. Endorsement noticeThe text of ISO/TS 10272-3:2010 has been approved by CEN as a CEN ISO/TS 10272-3:2010 without any modification.DD CEN ISO/TS 10272-3:2010CEN ISO/TS 10272-3:2010 (E)3 Foreword This d cu ent (CEN ISO/TS 10272-3:2010) has be
31、en prepared by Technical Committee ISO/TC 34 “Foodproducts“ in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” thesecretariat of wh ch is held by DIN.Attention is drawn to the possibility that some of the elements of this document may be the subject of patent r
32、ights. CEN and/or CENELEC shall not be held responsible f r identifying any or all such patent righ s. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the followingcountries are bound to announce this Technical Spe ification: Austria, Belgium, Bulgaria, Cro
33、atia, Cyprus, Czech Republic, Denm rk, Estonia, Finland, France, Germany, Greece, Hungary, Icel nd, Ireland, Italy,Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia,Spain, Sweden, Switzerland and the United Kingdom. Endorsement noticeThe text of
34、 ISO/TS 10272-3:2010 has been approved by CEN as a CEN ISO/TS 10272-3:2010 without any modification.DD CEN ISO/TS 10272-3:2010ISO/TS 10272-3:2010(E) ISO 2010 All rights reserved iiiContents PageForeword ivIntroduction.v1Scope.12Normative refrences.13Terms and efintions.14Principle25Culture media nd
35、reagents.26Apartus.37Sampling38Prepartion of test ample49Procedure.49.1 General .49.2 Test portion, initial suspension and dilutions49.3 Enrichment.49.4 Isolation49.5 Confirmation of Campylobacter spp.59.6 Identification of Campylobacter spp. (optional).610 Calculation and expression of results 810.
36、1 Method of calculation810.2 Precision.811 Test report9Annex A (normative) Diagram of procedure10Annex B (normative) Composition and preparation of culture media and reagents.11Bibliography17DD CEN ISO/TS 10272-3:2010ISO/TS 10272-3:2010(E)iv ISO 2010 All rights reservedForeword ISO (the Internationa
37、l Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has beenest
38、ablished has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardiz
39、ation. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2.The main task of technical committees is to prepare International Standards. Draft International Standardsadopted by the technical committees are circulated to the member bodies for voting
40、. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. In other circumstances, particularly when there is an urgent market requirement for such documents, a technical committee may decide to publish other types of document: an ISO Publicly
41、Available Specification (ISO/PAS) represents an agreement between technical experts inan ISO working group and is accepted for publication if it is approved by more than 50 % of the members of the parent committee casting a vote; an ISO Technical Specification (ISO/TS) represents an agreement betwee
42、n the members of a technicalcommittee and is accepted for publication if it is approved by 2/3 of the members of the committee casting a vote. An ISO/PAS or ISO/TS is reviewed after three years in order to decide whether it will be confirmed for afurther three years, revised to become an Internation
43、al Standard, or withdrawn. If the ISO/PAS or ISO/TS isconfirmed, it is reviewed again after a further three years, at which time it must either be transformed into an International Standard or be withdrawn. Attention is drawn to the possibility that some of the elements of this document may be the s
44、ubject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. ISO/TS 10272-3 was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 9, Microbiology. ISO 10272 consists of the following parts, under the general title Microbiology of f
45、ood and animal feeding stuffs Horizontal method for detection and enumeration of Campylobacter spp.: Part 1: Detection method Part 2: Colony count technique Technical Specification Part 3: Semi-quantitative method Technical SpecificationDD CEN ISO/TS 10272-3:2010ISO/TS 10272-3:2010(E) ISO 2010 All r
46、ights reserved vIntroduction Because of the large variety of food and feed products, ISO 10272 may not be appropriate in every detail forcertain products, and for some other products it may be necessary to use different methods. Nevertheless, in all cases, every attempt should be made to apply ISO 1
47、0272 as far as possible, anydeviations being made only if absolutely necessary for technical reasons.When ISO 10272 is next reviewed, account will be taken of all information then available regarding the extentto which the methods have been followed and the reasons for deviations from it in the case
48、 of particularproducts. The harmonization of test methods cannot be immediate and, for certain groups of products,International Standards and/or national standards may already exist that do not comply with ISO 10272. It ishoped that, when such standards are reviewed, they will be changed to comply w
49、ith ISO 10272, so that eventually the only remaining departures are those necessary for well-established technical reasons. DD CEN ISO/TS 10272-3:2010ISO/TS 10272-3:2010(E)iv ISO 2010 All rights reservedForeword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a technical committee has beenestablished has the
