1、raising standards worldwideNO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBSI Standards PublicationBS ISO 10710:2010Water quality Growth inhibition test with the marine and brackish water macroalga Ceramium tenuicorneBS EN ISO 10710:2013Copyright European Committee for Standar
2、dization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN ISO 10710:2013 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of EN ISO 10710:2013. It is identical to ISO 10710:2010. It supersede
3、s BS ISO 10710:2010, which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee EH/3, Water quality.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all the necessary pr
4、ovisions of a contract. Users are responsible for its correct application. The British Standards Institution 2013. Published by BSI Standards Limited 2013ISBN 978 0 580 79934 1ICS 13.060.70Compliance with a British Standard cannot confer immunity from legal obligations.This British Standard was publ
5、ished under the authority of the Standards Policy and Strategy Committee on 31 July 2010Amendments/corrigenda issued since publicationDate Text affected31 May 2013 This corrigendum renumbers BS ISO 10710:2010 as BS EN ISO 10710:2013Copyright European Committee for Standardization Provided by IHS und
6、er license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 10710 April 2013 ICS 13.060.70 English Version Water quality - Growth inhibition test with the marine and brackish water macroalga Ceramium t
7、enuicorne (ISO 10710:2010) Qualit de leau - Essai dinhibition de croissance sur la macro algue deaux marine et saumtre Ceramium tenuicorne (ISO 10710:2010) Wasserbeschaffenheit - Bestimmung der Wachstumshemmung auf die marine und stuarine Makroalge Ceramium tenuicorne (ISO 10710:2010) This European
8、Standard was approved by CEN on 14 March 2013. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerni
9、ng such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into i
10、ts own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germ
11、any, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR
12、 NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2013 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 10710:2013: ECopyright European Committee for Standardization Provided by IHS under license with CENNot for Resa
13、leNo reproduction or networking permitted without license from IHS-,-,-BS EN ISO 10710:2013EN ISO 10710:2013 (E)EN ISO 10710:2013 (E) 3 Foreword The text of ISO 10710:2010 has been prepared by Technical Committee ISO/TC 147 “Water quality” of the International Organization for Standardization (ISO)
14、and has been taken over as EN ISO 10710:2013 by Technical Committee CEN/TC 230 “Water analysis” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by October 201
15、3, and conflicting national standards shall be withdrawn at the latest by October 2013. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights
16、. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, G
17、ermany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 10710:2010 has been approved by CEN as EN ISO 1071
18、0:2013 without any modification. Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS ISO 10710:2010ISO 10710:2010(E) ISO 2010 All rights reserved iiiContents Page Foreword iv Intr
19、oduction.v 1 Scope1 2 Terms and definitions .1 3 Principle2 4 Test organisms, nutrients, media, and materials.2 5 Cultivation 6 6 Apparatus.6 7 Procedure.7 7.1 Algae material 7 7.2 Preparation of dilution water8 7.3 Choice of test concentrations8 7.4 Start of test.9 7.5 Incubation.9 7.6 Measurement
20、.9 7.7 Test with reference substances.9 8 Validity criteria.9 9 Evaluation of results .10 9.1 Calculations .10 9.2 Determination of ErCx.10 9.3 Expression of results10 9.4 Interpretation of results 11 10 Reproducibility 11 11 Test report11 Annex A (informative) Growth pattern of Ceramium tenuicorne
21、in 7S and 20S13 Annex B (informative) Results of ring test with Ceramium tenuicorne at 20 S.16 Bibliography17 BS ISO 10710:2010ISO 10710:2010(E) ISO 2010 All rights reserved iiiContents Page Foreword ivIntroduction.v1 Scope2 Terms and definitions .13 Principle4 Test organisms, nutrients, media, and
22、materials.25 Cultivation 6 Apparatus 67 Procedure.7 7.1 Algae material 7 7.2 Preparation of dilution water8 7.3 Choice of test concentrations8 7.4 Start of test.9 7.5 Incubation.9 7.6 Measurement .9 7.7 Test with reference substances.8 Validity criteria.99 Evaluation of results .10 9.1 Calculations
23、.10 9.2 Determination of ErCx.10 9.3 Expression of results10 9.4 Inte pretation of results 1110 Reproducibility 1111 Test report11A Growth pattern of Ceramium tenuicorne in 7S and 20S13Annex B (informative) Results of ring test with Ceramium tenuicorne at 20 S.16Bibliography17 BS EN ISO 10710:2013IS
24、O 10710:2010(E)Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS ISO 10710:2010ISO 10710:2010(E) ISO 2010 All rights reserved vIntroduction The red macroalga Ceramium tenuicorne
25、 belongs to Ceramiaceae, Rhodophyta. The species can be used as a model organism for the near coastal ecosystem. This species is found in temperate marine waters in both the northern and southern hemispheres and is thus relevant for large areas. As primary producers, they are a food source for many
26、invertebrates and serve as living habitat for bacteria, invertebrates, and juvenile fish. They also serve as substrate for many oviparous fish species. ivBS EN ISO 10710:2013ISO 10710:2010(E)Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo repro
27、duction or networking permitted without license from IHS-,-,-BS ISO 10710:2010INTERNATIONAL STANDARD ISO 10710:2010(E) ISO 2010 All rights reserved 1Water quality Growth inhibition test with the marine and brackish water macroalga Ceramium tenuicorne WARNING Persons using this International Standard
28、 should be familiar with normal laboratory practice. This International Standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user to establish appropriate safety and health practices and to ensure compliance with any nation
29、al regulatory conditions. IMPORTANT It is absolutely essential that tests conducted according to this International Standard be carried out by suitably trained staff. 1 Scope This International Standard specifies a method for the determination of the inhibition of growth of the macroalga Ceramium te
30、nuicorne by substances and mixtures contained in seawater or by waste water with salinities between 4S and 32S. This method is applicable to substances that are easily soluble in water. NOTE With modifications as described in ISO 144424and ISO 5667-162, the inhibitory effects of poorly soluble organ
31、ic and inorganic materials, volatile compounds, metals, waste water, marine water samples, and elutriates of sediments can be tested. 2 Terms and definitions For the purposes of this document, the following terms and definitions apply. 2.1 algal length length from the first division to the most dist
32、ant tip of the plant NOTE The algal length is expressed in millimetres. See Figure 1. 2.2 control medium combination of dilution water and/or nutrient medium used in the test ISO 20079:20055, 3.6 2.3 control batch control medium including organisms used for testing ISO 20079:20055, 3.5 2.4 effective
33、 concentration ErCxconcentration of test sample at which an effect of x % is measured, if compared to the control ISO 20079:20055, 3.9 BS EN ISO 10710:2013Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted with
34、out license from IHS-,-,-BS ISO 10710:2010ISO 10710:2010(E) 2 ISO 2010 All rights reserved2.5 growth medium mixture of natural seawater and nutrients in which algal plants are cultivated, which is used for pre-cultures NOTE Adapted from ISO 8692:20043, 3.3. 2.6 growth rate water quality proportional
35、 rate of increase in algal length per day NOTE See Clause 9. 2.7 salinity practical salinity S seawater ratio K15of the electrical conductivity of the seawater sample, at the temperature of 15 C and a pressure of one standard atmosphere, to that of a potassium chloride solutions in which the mass fr
36、action of KCl is 32,435 6 103, at the same temperature and pressure NOTE Adapted from Reference 14, p. 12. 2.8 test medium mixture of seawater, nutrients and test sample NOTE Adapted from ISO 8692:20043, 3.5. 2.9 test sample water quality aqueous sample, e.g. chemical substance, mixture of chemicals
37、 or waste water, for which the inhibitory effects on the growth of algae are determined NOTE Adapted from ISO 8692:20043, 3.4. 3 Principle Algal tips from monocultures of ceramium female gametophytes are grown in defined test conditions and in a defined medium containing a range of concentrations of
38、 the test sample. The test solutions are incubated for a period of 7 d after which the increase in length is measured and the growth rate is calculated. The growth inhibition is determined as a reduction in growth rate, relative to control cultures grown under identical conditions. When toxicity of
39、samples is to be compared to the toxicity of other chemicals or waste waters, tests can be performed in artificial seawater. If the purpose of the testing is to assess and to predict effects in a specific receiving water body, the tests can be conducted with algae adapted to the salinity in the rece
40、iving water body. In this case, natural seawater from an uncontaminated site of the same properties is used. 4 Test organisms, nutrients, media, and materials Unless otherwise stated, use only reagents of recognized analytical grade and distilled or demineralized water or water of equivalent purity.
41、 BS EN ISO 10710:2013ISO 10710:2010(E)Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS ISO 10710:2010ISO 10710:2010(E) ISO 2010 All rights reserved 34.1 Test organisms Use eith
42、er of the following marine algal clones: a) Ceramium tenuicorne Ktzing Waern (7S clone originating from the Baltic Sea); b) C. tenuicorne Ktzing Waern (20S and 30S clone originating from the Oslo fjord). This alga is a widely distributed macroalgae species (phylum Rhodophyta) in estuarine and coasta
43、l areas. The strains recommended are available in unialgal, non-axenic cultures1). NOTE 1 This growth inhibition test is based on two clones, which were formerly regarded as two different species. These species were the marine Ceramium strictum Harvey sensu Kylin and the brackish water species C. te
44、nuicorne Ktzing Waern. Complete interfertility (References 1112) and DNA data (Reference 10) have shown that the two entities belong to the same species, with C. tenuicorne as the valid name. The marine clone (former C. strictum) used in this test was isolated in 1973 and originates from the Oslo fj
45、ord (20S to 25S). It has been maintained as a laboratory culture for over 30 years. The brackish water clone was isolated in 1995 and originates from the Baltic Sea, 20 km south of the Ask laboratory in northern Baltic proper (6S to 7S). Cultures can be maintained in the medium specified in Clause 5
46、. Regular subculturing is necessary. NOTE 2 Among the red algae, changes occur between haploid and diploid generations. In the growth inhibition test, the female gametophytic generation is used since it has an even dichotomous growth pattern and the fastest growth rate. In nature it is difficult to
47、distinguish between male and female plants. This can be done in the laboratory where spermatangia are found on the branches of the male plants and trichogynes can be seen on the tips of the claws on the female plants. NOTE 3 The Baltic Sea clone can be adapted and used in tests in salinities between
48、 4S and 12S. The marine clone can be used as test organism in salinities between 12S and 32S. 4.2 Natural and artificial seawater 4.2.1 General Natural seawater is used for the cultivation of the algae and either natural or artificial seawater should be used for testing. The type of seawater to be used depends on the objective of the test. When natural seawater is used, care shall be taken to ensure that it is not polluted. Take special care to avoid contamination of the water by inorganic or organic substances during preparation and storage. Equipment made of copper
