1、raising standards worldwideNO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBSI Standards PublicationBS ISO 10869:2010Oil of fir needle, Siberian (Abies sibirica Ledeb.)BS ISO 10869:2010 BRITISH STANDARDNational forewordThis British Standard is the UK implementation of ISO 10869
2、:2010.The UK participation in its preparation was entrusted to TechnicalCommittee AW/54, Essential oils.A list of organizations represented on this committee can beobtained on request to its secretary.This publication does not purport to include all the necessaryprovisions of a contract. Users are r
3、esponsible for its correctapplication. BSI 2011ISBN 978 0 580 62697 5ICS 71.100.60Compliance with a British Standard cannot confer immunity fromlegal obligations.This British Standard was published under the authority of theStandards Policy and Strategy Committee on 28 February 2011.Amendments issue
4、d since publicationDate Text affectedBS ISO 10869:2010Reference numberISO 10869:2010(E)ISO 2010INTERNATIONAL STANDARD ISO10869First edition2010-11-15Oil of fir needle, Siberian (Abies sibirica Ledeb.) Huile essentielle de pin, Sibrie (Abies sibirica Ledeb.) BS ISO 10869:2010ISO 10869:2010(E) PDF dis
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8、TECTED DOCUMENT ISO 2010 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from either ISO at the address below or ISOs
9、member body in the country of the requester. ISO copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail copyrightiso.org Web www.iso.org Published in Switzerland ii ISO 2010 All rights reservedBS ISO 10869:2010ISO 10869:2010(E) ISO 2010 All rights rese
10、rved iiiForeword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for w
11、hich a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all
12、 matters of electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are c
13、irculated to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held resp
14、onsible for identifying any or all such patent rights. ISO 10869 was prepared by Technical Committee ISO/TC 54, Essential oils. BS ISO 10869:2010BS ISO 10869:2010INTERNATIONAL STANDARD ISO 10869:2010(E) ISO 2010 All rights reserved 1Oil of fir needle, Siberian (Abies sibirica Ledeb.) 1 Scope This In
15、ternational Standard specifies certain characteristics of the oil of fir needle, Siberian (Abies sibirica Ledeb.) in order to facilitate assessment of its quality. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references,
16、only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils General rules for labelling and marking of containers
17、ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 875, Essential oils Evaluation of miscibility in ethanol ISO 124
18、2, Essential oils Determination of acid value ISO 11024-1, Essential oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for presentation in standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromat
19、ographic profiles of samples of essential oils 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 oil of fir needle, Siberian essential oil obtained by steam distillation of the leaves (needles) and terminal branches of fir, Siberian (Abies sibi
20、rica Ledeb.) growing mainly in Siberia NOTE For information on CAS number, see ISO/TR 210923. BS ISO 10869:2010ISO 10869:2010(E) 2 ISO 2010 All rights reserved4 Specifications 4.1 Appearance Liquid. 4.2 Colour Colourless to pale yellow. 4.3 Odour Fresh, woody, resinous. 4.4 Relative density at 20 C,
21、 20d 20Minimum: 0,895 Maximum: 0,912 4.5 Refractive index at 20 C Minimum: 1,468 Maximum: 1,473 4.6 Optical rotation at 20 C Minimum: 25 Maximum: 40 4.7 Miscibility in 90 % (volume fraction) ethanol at 20 C It shall not be necessary to use more than 10 volumes of 90 % (volume fraction) ethanol to ob
22、tain a clear solution with 1 volume of essential oil. 4.8 Acid value Maximum: 1,0. 4.9 Chromatographic profile Carry out the analysis of the essential oil by gas chromatography. Identify in the chromatogram obtained, the representative and characteristic components shown in Table 1. The proportions
23、of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil. BS ISO 10869:2010ISO 10869:2010(E) ISO 2010 All rights reserved 3Table 1 Chromatographic profile Components Minimum (%) Maximum (%) Santene 1,5 3,5 Tricy
24、clene 1,5 3,5 -Pinene 10,0 22,0 Camphene 15,0 26,0 -Pinene 1,0 3,5 -3-Carene 9,0 15,0 Limonene 4,0 10,0 -Phellandrene 1,5 5,0 Bornyl acetate 20,0 35,0 -Caryophyllene 0,5 2,0 Isobornyl acetate n.d.a 0,1 Borneol 1,0 3,0 -Humulene 0,3 0,9 NOTE The chromatographic profile is normative, contrary to the t
25、ypical chromatograms given for information in Annex A. aNot detectable. 4.10 Flashpoint Information concerning the flashpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 25 ml. NOTE This volume is sufficient to carry out all the tests specified in this International S
26、tandard at least once. 6 Test methods 6.1 Relative density at 20 C, 20d 20See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. BS ISO 10869:2010ISO 10869:2010(E) 4 ISO 2010 All rights reserved6.4 Miscibility in 90 % (volume fraction) ethanol at 20 C See IS
27、O 875. 6.5 Acid value See ISO 1242. 6.6 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/TR 211. BS ISO 10869:2010ISO 10869:2010(E) ISO 2010 All rights reserved 5Annex A (informative) Typical chromatograms of the analysis by
28、gas chromatography of the essential oil of fir needle, Siberian (Abies sibirica Ledeb.) Peak identification Operating conditions 1 Santene 2 Tricyclene 3 -Pinene 4 Camphene 5 -Pinene 6 -3-Carene 7 Limonene 8 -Phellandrene 9 Borneol 10 Bornyl acetate 11 Isobornyl acetate 12 -Caryophllene 13 -Humulene
29、 Column: capillary, fused silica, 30 m length, 0,25 mm internal diameter Stationary phase: polydimethylsiloxane Film thickness: 0,25 m Oven temperature: temperature programming from 50 C to 230 C, at a rate of 2 C/min Injector temperature: 230 C Detector temperature: 250 C Detector: flame ionization
30、 type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/90 n counts t time Figure A.1 Typical chromatogram taken on an apolar column BS ISO 10869:2010ISO 10869:2010(E) 6 ISO 2010 All rights reservedPeak identification Operating conditions 1 Santene 2 Tricyclen
31、e 3 -Pinene 4 Camphene 5 -Pinene 6 -3-Carene 7 Limonene 8 -Phellandrene 9 Borneol 10 Bornyl acetate 11 Isobornyl acetate 12 -Caryophllene 13 -Humulene Column: capillary, 30 m long and 0,25 mm internal diameter Stationary phase: polyethylene glycol Carbowax BP201) Film thickness: 0,25 m Oven temperat
32、ure: temperature programming from 50 C to 230 C, at a rate of 2 C/min Injector temperature: 230 C Detector temperature: 250 C Detector: flame ionization type Carrier gas: helium Volume injected: 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/90 n counts t time Figure A.2 Typical chromatogram t
33、aken on a polar column 1) Carbowax BP20 is an example of a suitable product available commercially. This information is given for the convenience of users of this International Standard and does not constitute an endorsement by ISO of this product. BS ISO 10869:2010ISO 10869:2010(E) ISO 2010 All rig
34、hts reserved 7Annex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies, and people in charge of safety services require information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on th
35、e relevant methods of analysis (see ISO/TR 110182) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that: there is a wide variation in the chemical composition of essential oils; the volume of the sample needed for certain equipment is incompatible
36、with the high price of essential oils; as there are several different types of equipment which can be used for the determination, users cannot be expected to use one specified type only. Consequently, it was decided to give a mean value for the flashpoint annexed to each International Standard, for
37、information, in order to meet the requirements of the interested parties. The equipment with which this value was obtained has to be specified. For further information, see ISO/TR 110182. B.2 Flashpoint of the essential oil of fir needle, Siberian (Abies sibirica Ledeb.) The mean value is 43 C. NOTE
38、 Obtained with Grabner Instruments2)equipment. 2) Equipment available commercially. This information is given for the convenience of users of this International Standard and does not constitute an endorsement by ISO of this product. BS ISO 10869:2010ISO 10869:2010(E) 8 ISO 2010 All rights reservedBi
39、bliography 1 ISO 3960, Animal and vegetable fats and oils Determination of peroxide value Iodometric (visual) endpoint determination 2 ISO/TR 11018:1997, Essential oils General guidance on the determination of flashpoint 3 ISO/TR 21092, Essential oils Characterization BS ISO 10869:2010BS ISO 10869:2
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