1、BRITISH STANDARD BS ISO 3519:2005 Oil of lime distilled, Mexican type Citrus aurantifolia (Christm.) Swingle ICS 71.100.60 BS ISO 3519:2005 This British Standard was published under the authority of the Standards Policy and Strategy Committee on 12 January 2006 BSI 12 January 2006 ISBN 0 580 47139 X
2、 National foreword This British Standard reproduces verbatim ISO 3519:2005 and implements it The UK participation in its preparation was entrusted to Technical Committee AW/54, Essential oil, which has the responsibility to: aid enquirers to understand the text; present to the responsible internatio
3、nal/European committee any enquiries on the interpretation, or proposals for change, and keep UK interests informed; monitor related international and European developments and promulgate them in the UK. A list of organizations represented on this committee can be obtained on request to its secretar
4、y. Cross-references The British Standards which implement international publications referred to in this document may be found in the BSI Catalogue under the section entitled “International Standards Correspondence Index”, or by using the “Search” facility of the BSI Electronic Catalogue or of Briti
5、sh Standards Online. This publication does not purport to include all the necessary provisions of a contract. Users are responsible for its correct application. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a fr
6、ont cover, an inside front cover, the ISO title page, pages ii and iii, a blank page, pages 1 to 7 and a back cover. The BSI copyright notice displayed in this document indicates when the document was last issued. Amendments issued since publication Amd. No. Date Comments as the UK national standard
7、. Reference number ISO 3519:2005(E)INTERNATIONAL STANDARD ISO 3519 Third edition 2005-10-01 Oil of lime distilled, Mexican type Citrus aurantifolia (Christm.) Swingle Huile essentielle de limette distille, type Mexique Citrus aurantifolia (Christm.) Swingle BS ISO 3519:2005ii BS ISO 3519:2005 iii Fo
8、reword ISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a tec
9、hnical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters o
10、f electrotechnical standardization. International Standards are drafted in accordance with the rules given in the ISO/IEC Directives, Part 2. The main task of technical committees is to prepare International Standards. Draft International Standards adopted by the technical committees are circulated
11、to the member bodies for voting. Publication as an International Standard requires approval by at least 75 % of the member bodies casting a vote. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible fo
12、r identifying any or all such patent rights. ISO 3519 was prepared by Technical Committee ISO/TC 54, Essential oils. This third edition cancels and replaces the second edition (ISO 3519:1997), which has been technically revised. blank1 Oil of lime distilled, Mexican type Citrus aurantifolia (Christm
13、.) Swingle 1 Scope This International Standard specifies certain characteristics of the oil of lime distilled, Mexican type Citrus aurantifolia (Christm.) Swingle, in order to facilitate assessment of its quality. 2 Normative references The following referenced documents are indispensable for the ap
14、plication of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. ISO/TR 210, Essential oils General rules for packaging, conditioning and storage ISO/TR 211, Essential oils Gene
15、ral rules for labelling and marking of container ISO 212, Essential oils Sampling ISO 279, Essential oils Determination of relative density at 20 C Reference method ISO 280, Essential oils Determination of refractive index ISO 592, Essential oils Determination of optical rotation ISO 11024-1, Essent
16、ial oils General guidance on chromatographic profiles Part 1: Preparation of chromatographic profiles for presentation in standards ISO 11024-2, Essential oils General guidance on chromatographic profiles Part 2: Utilization of chromatographic profiles of samples of essential oils 3 Terms and defini
17、tions For the purposes of this document, the following terms and definitions apply. 3.1 oil of lime distilled volatile essential oil obtained by distillation from the juice or the crushed whole fruit of Citrus aurantifolia (Christm.) Swingle, of the Rutaceae family NOTE For information on the CAS nu
18、mber, see ISO/TR 21092. 4 Requirements 4.1 Appearance Mobile liquid, clear. 4.2 Colour Colourless to greenish yellow. 4.3 Odour Characteristic lime odour. 4.4 Relative density at 20 C, 20 20 d Minimum: 0,858 0 Maximum: 0,866 0 4.5 Refractive index at 20 C Minimum: 1,474 0 Maximum: 1,477 0 4.6 Optica
19、l rotation at 20 C Between +31 and +42. BS ISO 3519:2005BS ISO 3519:2005 2 4.7 Chromatographic profile Analysis of the essential oil shall be carried out by gas chromatography. In the chromatogram obtained, the representative and characteristic components shown in Table 1 shall be identified. The pr
20、oportions of these components, indicated by the integrator, shall be as shown in Table 1. This constitutes the chromatographic profile of the essential oil. Table 1 Chromatographic profile Component Minimum % Maximum % -Pinene 0,8 1,3 Sabinene 0,1 0,3 -Pinene 1,0 3,0 Myrcene 1,1 1,5 p-Cymene 1,5 2,8
21、 Limonene a36,0 46,0 -Terpinene 10,0 13,0 Fenchol 0,4 0,8 Borneol b0,5 0,8 -Terpineol 6,0 8,0 -Terpineol 0,7 1,4 -Caryophyllene 0,4 0,8 -Bergamotene 0,5 0,9 -Farnesene 0,6 0,9 -Bisabolene 1,0 1,5 aLimonene is regarded to be predominantly D-limonene based on physical tests. It is believed that there
22、might be a small amount of L-limonene present but the exact quantity is unknown. bBorneol is regarded to be predominantly D-borneol based on physical tests. It is believed that there might be a small amount of L-borneol present but the exact quantity is unknown. NOTE The chromatographic profile is n
23、ormative, contrary to typical chromatograms given for information in Annex A. 4.8 Flashpoint Information on the flashpoint is given in Annex B. 5 Sampling See ISO 212. Minimum volume of test sample: 25 ml. NOTE This volume allows each of the tests specified in this International Standard to be carri
24、ed out at least once. 6 Test methods 6.1 Relative density at 20 C, 20 20 d See ISO 279. 6.2 Refractive index at 20 C See ISO 280. 6.3 Optical rotation at 20 C See ISO 592. 6.4 Chromatographic profile See ISO 11024-1 and ISO 11024-2. 7 Packaging, labelling, marking and storage See ISO/TR 210 and ISO/
25、TR 211. 3 Annex A (informative) Typical chromatograms of the analysis by gas chromatography of the oil of lime distilled, Mexican type Citrus aurantifolia (Christm.) Swingle BS ISO 3519:20054 Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 -Pinene Sabinene -Pinene Myrcene p-Cymene Limonen
26、e -Terpinene Terpinolene Fenchol 10 11 12 13 14 15 16 17 Borneol Terpinen-4-ol -Terpineol -Terpineol -Caryophyllene -Bergamotene -Farnesene -Bisabolene Column: fused silica; length 30 m; internal diameter 0,25 mm Stationary phase: poly(dimethyl siloxane) (DB-1 ) Film thickness: 0,25 m Oven temperatu
27、re: isothermal at 75 C for 5 min, then temperature programming from 75 C to 100 C at a rate of 5 C/min, then from 100 C to 220 C at a rate of 6 C/min and isothermal at 220 C until all components have eluted Injector temperature: 230 C Detector temperature: 260 C Detector: flame ionization type Carri
28、er gas: helium Volume injected: 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/100 Figure A.1 Typical chromatogram taken on an apolar column BS ISO 3519:20055Peak identification Operating conditions 1 2 3 4 5 6 7 8 9 -Pinene -Pinene Sabinene Myrcene Limonene -Terpinene p-Cymene Terpinolene Fen
29、chol 10 11 12 13 14 15 16 17 -Bergamotene Terpinen-4-ol -Caryophyllene -Terpineol -Terpineol Borneol -Bisabolene -Farnesene Column: fused silica; length 30 m; internal diameter 0,25 mm Stationary phase: poly(ethylene glycol) (DB-WAX ) Film thickness: 0,25 m Oven temperature: isothermal at 75 C for 5
30、 min, then temperature programming from 75 C to 100 C at a rate of 5 C/min, then from 100 C to 220 C at a rate of 6 C/min and isothermal at 220 C until all components have eluted Injector temperature: 230 C Detector temperature: 260 C Detector: flame ionization type Carrier gas: helium Volume inject
31、ed: 0,1 l Carrier gas flow rate: 1 ml/min Split ratio: 1/100 Figure A.2 Typical chromatogram taken on a polar column BS ISO 3519:20056 Annex B (informative) Flashpoint B.1 General information For safety reasons, transport companies, insurance companies, and people in charge of safety services requir
32、e information on the flashpoints of essential oils, which in most cases are flammable products. A comparative study on the relevant methods of analysis (see ISO/TR 11018) concluded that it was difficult to recommend a single apparatus for standardization purposes, given that: there is a wide variati
33、on in the chemical composition of essential oils; the volume of the sample needed to meet certain requirements would be too costly for high-priced essential oils; as there are several different types of equipment which can be used for the determination, users cannot be expected to use one specified
34、type only. Consequently, it was decided to give a mean value for the flashpoint in an informative annex to each International Standard, in order to meet the requirements of the interested parties. The equipment with which this value was obtained should be specified. For further information, see ISO/
35、TR 11018. B.2 Flashpoint of the oil of lime distilled, Mexican type The mean value is +46 C. NOTE Obtained with “Setaflash“ equipment. BS ISO 3519:20057 Bibliography 1 ISO/TR 11018, Essential oils General guidance on the determination of flashpoint 2 ISO/TR 21092, Essential oils Characterization BS
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