1、March 2010 Translation by DIN-Sprachendienst.English price group 13No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS
2、13.060.70!$a6?“1621928www.din.deDDIN EN 15708Water quality Guidance standard for the surveying, sampling and laboratory analysisof phytobenthos in shallow running waterEnglish translation of DIN EN 15708:2010-03Wasserbeschaffenheit Anleitung zur Beobachtung, Probenahme und Laboranalyse von Phytobent
3、hos in flachenFliegewssernEnglische bersetzung von DIN EN 15708:2010-03Qualit de leau Guide pour ltude, lchantillonnage et lanalyse en laboratoire du phytobenthos dans lescours deau peu profondsTraduction anglaise de DIN EN 15708:2010-03www.beuth.deDocument comprises pagesIn case of doubt, the Germa
4、n-language original shall be considered authoritative.2603.10 DIN EN 15708:2010-03 A comma is used as the decimal marker. National foreword This standard has been prepared by Technical Committee CEN/TC 230 “Water analysis” (Secretariat: DIN, Germany). The responsible German body involved in its prep
5、aration was the Normenausschuss Wasserwesen (Water Practice Standards Committee), Working Committees NA 119-01-03-05-06 AK Biologisch-kologische Gewsseruntersuchung and NA 119-01-03 AA Wasseruntersuchung. This standard is part of the series Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schla
6、mmuntersuchung Biologisch-kologische Gewsseruntersuchung (Gruppe M) (German standard methods for the examination of water, waste water and sludge Biological-ecological methods of analysis (group M) and describes method M 32. Designation of method M 32 “Guidance on the surveying, sampling and laborat
7、ory analysis of phytobenthos in shallow running water“: Method DIN EN 15708 M 32 This standard has been published to implement the Water Framework Directive (WFD), Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in t
8、he field of water policy1). 1)Registered in the DITR database of DIN Software GmbH, obtainable from: Beuth Verlag GmbH, 10772 Berlin. 2 DIN EN 15708:2010-03 Expert assistance and specialized laboratories will be required to perform the analysis described in this standard. Existing safety requirement
9、s are to be observed. Depending on the objective of the analysis, a check shall be made on a case-by-case basis as to whether and to what extent additional conditions will have to be specified. German standard methods for the examination of water, waste water and sludge Standard methods published as
10、 DIN Standards are obtainable from Beuth Verlag GmbH, either individually or grouped in volumes. The standard methods included in the loose-leaf publication entitled Deutsche Einheits-verfahren zur Wasser-, Abwasser- und Schlammuntersuchung will continue to be published by Wiley-VCH Verlag and Beuth
11、 Verlag GmbH. All standard methods relevant to the Abwasserverordnung (Waste Water Regulation) (AbwV)2) included in the new Regulation on Section 7a of the Gesetz zur Ordnung des Wasserhaushalts (German Water Management Act)2)concerning Anforderungen an das Einleiten von Abwasser in Gewsser together
12、 with the Abwasserverordnung and the Gesetz zur Ordnung des Wasserhaushalts have been published by Beuth Verlag GmbH as a loose-leaf collection Analysenverfahren in der Abwasserverordnung Rechtsvorschriften und Normen (Supplements). Standards or draft standards bearing the group title “German standa
13、rd methods for the examination of water, waste water and sludge” are classified under the following categories (main titles): General information (group A) (DIN 38402) Sensory analysis (group B) (DIN 38403) Physical and physicochemical parameters (group C) (DIN 38404) Anions (group D) (DIN 38405) Ca
14、tions (group E) (DIN 38406) Substance group analysis (group F) (DIN 38407) Gaseous constituents (group G) (DIN 38408) Parameters characterizing effects and substances (group H) (DIN 38409) Biological-ecological methods of analysis (group M) (DIN 38410) Microbiological methods (group K) (DIN 38411) T
15、est methods using water organisms (group L) (DIN 38412) Individual constituents (group P) (DIN 38413) Sludge and sediments (group S) (DIN 38414) Bio-assays with microorganisms (group T) (DIN 38415) In addition to the methods described in the DIN 38402 to DIN 38415 series of standards, there are a nu
16、mber of European and International Standards available as DIN EN, DIN EN ISO and DIN ISO Standards which also form part of the collection of German standard methods. Information on Parts of these series of standards that have already been published can be obtained from the offices of the Normenaussc
17、huss Wasserwesen, telephone +49 (30) 2601-2448, or from Beuth Verlag GmbH, Burggrafenstrae 6, 10787 Berlin, Germany. 2)Registered in the DITR database of DIN Software GmbH, obtainable from: Beuth Verlag GmbH, 10772 Berlin. 3 DIN EN 15708:2010-03 4 This page is intentionally blank EUROPEAN STANDARD N
18、ORME EUROPENNE EUROPISCHE NORM EN 15708 November 2009 ICS 13.060.70 English Version Water quality - Guidance standard for the surveying, sampling and laboratory analysis of phytobenthos in shallow running waterQualit de leau - Guide pour ltude, lchantillonnage et lanalyse en laboratoire du phytobent
19、hos dans les cours deau peu profonds Wasserbeschaffenheit - Anleitung zur Beobachtung, Probenahme und Laboranalyse von Phytobenthos in flachen Fliegewssern This European Standard was approved by CEN on 10 October 2009. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which s
20、tipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member. This European Stand
21、ard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions. CEN members are the national sta
22、ndards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United K
23、ingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2009 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 15708:2009: EEN
24、15708:2009 (E) 2 Contents Page Foreword 3Introduction .41 Scope 52 Normative references 53 Terms and definitions .54 Principle 85 Reagents .86 Equipment 87 Survey and sampling strategy . 108 Survey and sampling procedures . 129 Identification and basic quantification of organisms . 1710 Data process
25、ing and interpretation 1811 Quality assurance . 19Annex A (informative) Worked example of computation of final abundance estimates for a MHS from an upland stream in NE England . 20Bibliography . 21DIN EN 15708:2010-03 EN 15708:2009 (E) 3 Foreword This document (EN 15708:2009) has been prepared by T
26、echnical Committee CEN/TC 230 “Water analysis”, the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by May 2010, and conflicting national standards shall be withd
27、rawn at the latest by May 2010. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. According to the CEN/CENELEC Internal Regulations, the
28、 national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Nor
29、way, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. DIN EN 15708:2010-03 EN 15708:2009 (E) 4 Introduction WARNING Working in or around water is inherently dangerous. Persons using this European Standard should be familiar with normal laboratory prac
30、tice. Long periods of analysis at the microscope can cause physical fatigue and affect eyesight. Attention should be given to the ergonomics of the microscope and advice from a health and safety practitioner should be sought to ensure that risks are minimized. The use of chemical products mentioned
31、in this standard can be hazardous and users should follow guidelines provided by the manufacturers and take necessary specialist advice. This standard does not purport to address the safety problems associated with its use. It is the responsibility of the user to establish appropriate health and saf
32、ety practices and to ensure compliance with any national regulatory conditions. The phytobenthos is an important component of aquatic ecosystems and an understanding of the composition of the phytobenthos present in a waterbody can provide useful information on the status of that waterbody, and on a
33、ppropriate management strategies. The Water Framework Directive (2000/60/EC) 3 requires monitoring of the phytobenthos as a quality element used for ecological status assessment, and phytobenthos assessments have also been used in monitoring programmes associated with other European Directives (e.g.
34、 Urban Wastewater Treatment Directive, Habitats Directive) and with national legislation (e.g. NORM M6231). This guidance standard specifically relates to the sampling of phytobenthos (other than aquatic macrophytes) in running water. An etymologically-correct application of the term “phytobenthos”
35、would cover all phototrophic organisms; however, this encompasses a vast range of organisms, from microscopic unicells to macrophytes 2 m in length. As separate survey methods for aquatic macrophytes are available (EN 14184), this document focuses on phototrophic algae and oxygenic cyanobacteria tha
36、t live on substrata. Bryophytes are common in shallow running waters and competitive interactions between these and larger algae are common. Similarly, aquatic macrophyte species may, themselves, act as substrata or competitors for algae and cyanobacteria. For these reasons, the standard provides op
37、tions for including these taxa in survey and sampling procedures. The term “periphyton” is sometimes used instead of “phytobenthos”; however, some definitions of “periphyton” include heterotrophic organisms that live attached to substrata (protozoa, sponges, hydroides). Methods described here deal o
38、nly with photosynthetic organisms but they could, if required, be adapated to encompass heterotrophic organisms too. Methods using phytobenthos to assess water quality in running water have been developed in several European countries 6, 8, 9, 10 and in the USA 2. Recent work is summarised in the pr
39、oceedings of four symposia 1, 7, 11, 12. Methods for the sampling and analysis of one group of phytobenthos, the diatoms, have already been the subject of harmonisation (EN 13946, EN 14407). However, these standards are concerned with only a single group of the phytobenthos and there are situations
40、where other phototrophs are more obvious and can contribute additional ecological information. According to the precise usage to which this standard is to be put it is essential for specifiers and users to mutually agree on any necessary variations or optional procedural details prior to use. DIN EN
41、 15708:2010-03 EN 15708:2009 (E) 5 1 Scope This European Standard provides guidelines for the survey/sampling, identification and basic quantification of phytobenthos (other than macrophytes) in running waters. It is applicable to rivers where benthic algae and bryophytes are the main phototrophs. T
42、his method encompasses all phytobenthic growth forms and enables biological responses to environmental events over one or more years to be monitored. In this respect it provides an alternative to methods based on benthic diatoms (EN 13946; EN 14407) and macrophytes (EN 14184). Data obtained for the
43、phytobenthos growth forms are suitable for pilot surveys, water quality assessment and trend monitoring. This European Standard encompasses all aspects from the design of survey and sampling programmes to the identification and basic quantification of the phytobenthos. 2 Normative references The fol
44、lowing referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN 13946, Water quality Guidance standard for the routin
45、e sampling and pretreatment of benthic diatoms from rivers EN 14407, Water quality Guidance standard for the identification, enumeration and interpretation of benthic diatom samples from running waters EN 15204, Water quality Guidance standard on the enumeration of phytoplankton using inverted micro
46、scopy (Utermhl technique) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 aquatic macrophytes larger plants of fresh water which are easily seen with the naked eye, including all aquatic vascular plants, bryophytes, stoneworts (Characeae) and
47、 macro-algal growths EN 14184:2003, 3.1 3.2 assemblage organisms that share a sampling area NOTE This term is preferred to “community”, as the latter implies a level of ecological integration of the organisms; whereas sampling may inadvertently combine representatives from more than one true “commun
48、ity” that are not distinct to the naked eye. 3.3 belt transect defined band across a river or stream at right angles to the bank NOTE This may be virtual or physically delineated within which the aquatic vegetation is analysed (species composition, abundance, cover). EN 14184:2003, 3.4 DIN EN 15708:
49、2010-03 EN 15708:2009 (E) 6 3.4 benthic alga alga or oxygenic cyanobacterium living attached to a substratum (rather than suspended in the water column) 3.5 biofilm mucilagineous polysaccharide matrix on submerged stable surfaces consisting of photo(auto)trophic and heterotrophic organisms 3.6 boulder mineral substratum with a diameter 256 mm EN 13946:2003, 3.3 3.7 bryophyte collective term for liverworts and mosses plants which are often abundant
