1、November 2016 English price group 13No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 71.100.50!%ue“2588266www.din.de
2、DIN EN 46-1Wood preservatives Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 1: Application by surface treatment (laboratory method);English version EN 461:2016,English translation of DIN EN 46-1:2016-11Holzschutzmittel Bestimmung der vor
3、beugenden Wirkung gegenber frisch geschlpften Larven von Hylotrupes bajulus (Linnaeus) Teil 1: Anwendung durch Oberflchenverfahren (Laboratoriumsverfahren);Englische Fassung EN 461:2016,Englische bersetzung von DIN EN 46-1:2016-11Produits de prservation du bois Dtermination de laction prventive cont
4、re les larves rcemment closes dHylotrupes bajulus (Linnaeus) Partie 1: Application par traitement de surface (Mthode de laboratoire);Version anglaise EN 461:2016,Traduction anglaise de DIN EN 46-1:2016-11SupersedesDIN EN 461:201003www.beuth.deDocument comprises 28 pagesDTranslation by DIN-Sprachendi
5、enst.In case of doubt, the German-language original shall be considered authoritative.11.16 DIN EN 46-1:2016-11 2 A comma is used as the decimal marker. National foreword This document (EN 46-1:2016) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and wood-based products” (Sec
6、retariat: AFNOR, France). The responsible German body involved in its preparation was DIN-Normenausschuss Holzwirtschaft und Mbel (DIN Standards Committee Timber and Furniture), Working Committee NA 042-03-06 AA Spiegelausschuss zu CEN/TC 38 und ISO/TC 165/SC 1 Dauerhaftigkeit von Holz und Holzprodu
7、kten. Amendments This standard differs from DIN EN 46-1:2010-03 as follows: a) the requirements for wood quality have been unified; b) it is now possible to omit control test specimens treated with a solvent or diluent when the solvent or diluent is water of drinking quality; c) for determining the
8、validity of the test, an X-ray apparatus has been added in Subclause 8.4.2. Previous editions DIN 52621: 1949-06 DIN 52163: 1952-08 DIN 52163-1: 1973-01 DIN EN 46: 1977-02, 1990-04 DIN EN 46-1: 2005-06, 2010-03 EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 46-1 June 2016 ICS 71.100.50 Superse
9、des EN 46-1:2009English Version Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 1: Application by surface treatment (laboratory method) Produits de prservation du bois - Dtermination de laction prventive contre les l
10、arves rcemment closes dHylotrupes bajulus (Linnaeus) - Partie 1: Application par traitement de surface (Mthode de laboratoire) Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenber frisch geschlpften Larven von Hylotrupes bajulus (Linnaeus) - Teil 1: Anwendung durch Oberflchenverfahren (La
11、boratoriumsverfahren) This European Standard was approved by CEN on 5 January 2017. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists an
12、d bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the
13、responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugosla
14、v Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE
15、 NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2016 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 46-1:2016 E2 Contents Page European foreword . 4 Introduction 5 1 S
16、cope 6 2 Normative references 6 3 Terms and definitions . 6 4 Principle . 7 5 Test materials . 7 5.1 Biological material 7 5.2 Products and reagents . 7 5.3 Apparatus . 7 6 Sampling . 8 7 Test specimens . 8 7.1 Species of wood 8 7.2 Wood quality . 9 7.3 Provision of test specimens . 9 7.4 Dimensions
17、 of test specimens 9 7.5 Number of test specimens 9 8 Procedure 10 8.1 Preparation of the test specimens . 10 8.1.1 Conditioning of the test specimens prior to sealing 10 8.1.2 Sealing of the transverse faces 10 8.1.3 Treatment of the test specimens 10 8.1.4 Drying and conditioning of the test speci
18、mens after treatment . 12 8.2 Exposure of the test specimens to the insects . 12 8.3 Conditions and duration of the test . 13 8.4 Examination of the test specimens 13 8.4.1 Examination . 13 8.4.2 Validity of the test 14 9 Expression of results . 14 9.1 Evaluation of attack . 14 9.2 Toxic values 14 1
19、0 Test report 15 Annex A (informative) Example of a test report 17 Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus) 19 B.1 General . 19 B.2 Obtaining parent beetles . 19 B.3 Mating . 19 B.4 Egg-Iaying 19 EN 46-1:2016 (E) DIN EN 46-1:2016-11 3 B.5 Hatching of eggs 20 B.6 La
20、rval development . 20 B.7 Enemies and parasites 21 Annex C (informative) Differentiation of heartwood and sapwood in Pinus species . 22 C.1 Principle . 22 C.2 Reagents . 22 C.3 Apparatus 22 C.4 Procedure 22 Annex D (informative) Environmental, health and safety precautions within chemical/biological
21、 laboratory . 23 Annex E (normative) Slow acting and deferred effect formulations Extending the test duration . 24 Bibliography . 26 EN 46-1:2016 (E) DIN EN 46-1:2016-11 4 European foreword This document (EN 46-1:2016) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and wood-b
22、ased products”, the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2016, and conflicting national standards shall be withdrawn at the latest by Dec
23、ember 2016. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN 46-1:2009. Significant technical differences b
24、etween this document and EN 46-1:2009 are as follows: a) introduction of new harmonized specifications for wood quality; b) option to omit control test specimens treated with the solvent or diluents only when the solvent or diluents is water of drinking quality; c) determination of validity of the t
25、est by using an X-ray apparatus added in 8.4.2. The standard EN 46 is composed of two parts: EN 46-1, Wood preservatives Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 1: Application by surface treatment (laboratory method) EN 46-2, Wood
26、preservatives Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 2: Ovicidal effect (laboratory method) EN 46 consists of two parts to enable preventive action of wood preservatives, against recently hatched larvae of Hylotrupes bajulus, whic
27、h are intended to be applied by surface treatment; Part 1 is required to determine the larvicidial effect of preservatives and Part 2 is required to determine the ovicidal action of the preservatives after egg-laying of young females. According to the CEN/CENELEC Internal Regulations, the national s
28、tandards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania
29、, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. EN 46-1:2016 (E) DIN EN 46-1:2016-11 5 Introduction This European Standard describes a laboratory method of testing which gives a basis for the assessme
30、nt of the preventive action of a wood preservative, when applied as a surface treatment, against recently hatched larvae of Hylotrupes bajulus, whereas the method for determining the toxic values against Hylotrupes bajulus (EN 47) provides a means of checking whether a preservative prevents attack b
31、y these larvae and prevents their survival within totally impregnated wood. This method makes it possible to determine whether recently hatched larvae are capable of boring through the treated surface of a susceptible wood species and of surviving in the untreated part of the test specimen. For this
32、 purpose, the procedure seeks to reproduce normal egg-laying conditions existing in cracks in wood, which provide the principal egg-laying sites. It takes account of the fact that, if larvae pass through the treated surface, they will then tunnel in the direction of the least protected regions of th
33、e wood. This laboratory method provides one criterion by which the value of a preservative can be assessed. In making this assessment, the methods by which the preservative may be applied should be taken into account. This test is of particular interest when applied to test specimens which have been
34、 subjected to an ageing procedure. It is further recommended that results from this test should be supplemented by those from other appropriate tests and, above all, by practical experience. When products which are very active at low concentrations are used it is very important to take suitable prec
35、autions to isolate and separate, as far as possible, operations involving chemical products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special tr
36、aining for personnel (see also Annex D for environmental, health and safety precautions). EN 46-1:2016 (E) DIN EN 46-1:2016-11 6 1 Scope This European Standard specifies a method for the determination of the preventive action of a wood preservative against recently hatched larvae of Hylotrupes bajul
37、us (Linnaeus) when the preservative is applied as a surface treatment to wood. This method is applicable to: water-insoluble chemicals which are being studied as active insecticides; organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates; organic water-dispers
38、ible formulations as supplied or as prepared in the laboratory by dilution of concentrates; and water-soluble materials, for example salts. The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures. 2 Normative references The following documents,
39、 in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN 73, Wood preservatives Accel
40、erated ageing of treated wood prior to biological testing Evaporative ageing procedure EN 84, Wood preservatives Accelerated ageing of treated wood prior to biological testing Leaching procedure EN ISO 3696, Water for analytical laboratory use Specification and test methods (ISO 3696) 3 Terms and de
41、finitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample having its physical and/or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled SOURCE: EN 1001-2:2005, 4.71 3.2 supplier
42、 sponsor of the test (person or company providing the sample of wood preservative to be tested) Note 1 to entry: Adapted from EN 10012:2005, 4.83. EN 46-1:2016 (E) DIN EN 46-1:2016-11 7 4 Principle Depending on the test being carried out either: on a set of test specimens of a susceptible wood speci
43、es that is surface treated with a solution of the preservative; or if toxic values are to be determined, on several sets of test specimens of a susceptible wood species that are surface treated with a series of solutions in which the concentration of preservative is ranged in a given progression. Th
44、e treated test specimens are exposed to recently hatched larvae of Hylotrupes bajulus. The resulting attack is observed and compared with those in untreated control test specimens. If the preservative has been prepared in the laboratory by dilution of a concentrate or by dissolution of a solid, the
45、resulting attack is also compared to that in solvent or diluent treated control test specimens. 5 Test materials 5.1 Biological material 5.1.1 Hylotrupes bajulus (Linnaeus) larvae, within three days of hatching. 5.1.2 Source of larvae. Obtain the larvae from cultures reared, e.g. as described in Ann
46、ex B. 5.1.3 Provision of larvae. Collect larvae from eggs laid by different females. 5.1.4 Choice of larvae. Use a mixed batch of these larvae for the test. Use ten larvae per treated test specimen or control test specimen. 5.2 Products and reagents 5.2.1 Paraffin wax, for fixing the glass plate and
47、 for sealing the end faces of test specimens to be treated with solutions in all cases in which water is the continuous phase. NOTE Paraffin wax with a setting point of 52 C to 54 C has been found to be suitable. 5.2.2 Gelatin, for sealing the end faces of test specimens to be treated with solutions
48、 in which an organic solvent is the continuous phase. 5.2.3 Water, complying with grade 3 of EN ISO 3696. 5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a
49、 toxic effect on the insects. CAUTION Do not use benzene or other solvents which pose a health risk. 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, and controlled at (28 2) C and at a relative humidity of (70 5) %. 5.3.2 Conditioning chamber, well ventilated and controlled at (20 2) C and at a relative humidity of (65 5) %. EN 46-1:2016 (E) DIN EN 46-1:2016-11 8 The conditioning of test specimens may b
