1、July 2012 Translation by DIN-Sprachendienst.English price group 11No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 1
2、3.060.70!$,u“1900982www.din.deDDIN EN 16150Water quality Guidance on pro-rata Multi-Habitat sampling of benthicmacro-invertebrates from wadeable riversEnglish translation of DIN EN 16150:2012-07Wasserbeschaffenheit Anleitung fr die pro-rata Multi-Habitat-Probenahme benthischer Makroinvertebraten inF
3、lssen geringer Tiefe (watbar)Englische bersetzung von DIN EN 16150:2012-07Qualit de leau Lignes directrices pour lchantillonnage des macroinvertbrs benthiques en cours deaupeu profonds au prorata des surfaces de recouvrement des habitats prsentsTraduction anglaise de DIN EN 16150:2012-07www.beuth.de
4、Document comprises 18 pagesIn case of doubt, the German-language original shall be considered authoritative.06.12 DIN EN 16150:2012-07 2 A comma is used as the decimal marker. This standard has been prepared by Technical Committee CEN/TC 230 “Water analysis” (Secretariat: DIN, Germany). The responsi
5、ble German body involved in its preparation was the Normenausschuss Wasserwesen (Water Practice Standards Committee), Working Committee NA 119-01-03-05-06 AK Biologisch-kologische Gewsseruntersuchung of NA 119-01-03 AA Wasseruntersuchung. This standard is part of the series Deutsche Einheitsverfahre
6、n zur Wasser-, Abwasser- und Schlammuntersuchung Biologisch-kologische Gewsseruntersuchung (Gruppe M) (German standard methods for the examination of water, waste water and sludge Biological-ecological methods of analysis (group M) and describes method M 70. Designation of method M 70 “Guidance on p
7、ro-rata Multi-Habitat sampling of benthic macro-invertebrates from wadeable rivers”: Method DIN EN 16150 M 70 This standard has been published to implement the Water Framework Directive (WFD), Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framew
8、ork for Community action in the field of water policy1). Expert assistance and specialized laboratories will be required to perform the analysis described in this standard. Existing safety requirements are to be observed. Depending on the objective of the analysis, a check shall be made on a case-by
9、-case basis as to whether and to what extent additional conditions will have to be specified. German standard methods for the examination of water, waste water and sludge Standard methods published as DIN Standards are obtainable from Beuth Verlag GmbH, either individually or grouped in volumes. The
10、 standard methods included in the loose-leaf publication entitled Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammuntersuchung will continue to be published by Wiley-VCH Verlag and Beuth Verlag GmbH. All standard methods relevant to the Abwasserverordnung (Waste Water Regulation) (AbwV)
11、1) included in the new Regulation on Section 57, paragraph 1, number 1, of the Gesetz zur Ordnung des Wasserhaushalts (German Water Management Act)1)concerning Anforderungen an das Einleiten von Abwasser in Gewsser together with the Abwasserverordnung and the Gesetz zur Ordnung des Wasserhaushalts h
12、ave been published by Beuth Verlag GmbH as a loose-leaf collection Analysenverfahren in der Abwasserverordnung Rechtsvorschriften und Normen (Supplements). 1) Registered in the DITR database of DIN Software GmbH, obtainable from: Beuth Verlag GmbH, 10772 Berlin. National foreword DIN EN 16150:2012-0
13、7 3 Standards or draft standards bearing the group title “German standard methods for the examination of water, waste water and sludge” are classified under the following categories (main titles): General information (group A) (DIN 38402) Sensory analysis (group B) (DIN 38403) Physical and physicoch
14、emical parameters (group C) (DIN 38404) Anions (group D) (DIN 38405) Cations (group E) (DIN 38406) Substance group analysis (group F) (DIN 38407) Gaseous constituents (group G) (DIN 38408) Parameters characterizing effects and substances (group H) (DIN 38409) Biological-ecological methods of analysi
15、s (group M) (DIN 38410) Microbiological methods (group K) (DIN 38411) Test methods using water organisms (group L) (DIN 38412) Individual constituents (group P) (DIN 38413) Sludge and sediments (group S) (DIN 38414) Bio-assays with microorganisms (group T) (DIN 38415) In addition to the methods desc
16、ribed in the DIN 38402 to DIN 38415 series of standards, there are a number of European and International Standards available as DIN EN, DIN EN ISO and DIN ISO Standards which also form part of the collection of German standard methods. Information on Parts of these series of standards that have alr
17、eady been published can be obtained from the offices of the Normenausschuss Wasserwesen, telephone +49 (30) 2601-2448, or from Beuth Verlag GmbH, Burggrafenstrae 6, 10787 Berlin, Germany. DIN EN 16150:2012-07 4 This page is intentionally blank EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 161
18、50 April 2012 ICS 13.060.70 English Version Water quality - Guidance on pro-rata Multi-Habitat sampling of benthic macro-invertebrates from wadeable rivers Qualit de leau - Lignes directrices pour lchantillonnage des macroinvertbrs benthiques en cours deau peu profonds au prorata des surfaces de rec
19、ouvrement des habitats prsents Wasserbeschaffenheit - Anleitung fr die pro-rata Multi-Habitat-Probenahme benthischer Makroinvertebraten in Flssen geringer Tiefe (watbar) This European Standard was approved by CEN on 16 March 2012. CEN members are bound to comply with the CEN/CENELEC Internal Regulat
20、ions which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member.
21、 This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN
22、members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Sp
23、ain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2012 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN n
24、ational Members. Ref. No. EN 16150:2012: EEN 16150:2012 (E) 2 Contents Page Foreword 31 Scope 42 Normative references 43 Terms and definitions .44 Description of the sampling approach 65 Field sampling procedures .75.1 Factors limiting effective Multi-Habitat-Sampling (MHS).75.2 Estimation of habita
25、t composition .75.3 Allocation of sampling units .76 Detailed description of sampling procedures 86.1 General recommendations for sampling .86.2 Megalithal (bedrock and boulders) 96.3 Macrolithal and mesolithal (cobbles, stones, pebbles) .96.4 Microlithal and smaller mineral substrates .96.5 Xylal (
26、woody debris) 96.6 Roots 106.7 Coarse Particulate Organic Matter (CPOM/leaf litter) . 106.8 Macrophytes (emergent and submerged) 106.9 Technolithal . 107 Sample treatment 107.1 Removal of large material and sorting . 107.2 Removal of large organisms 107.3 Storage . 107.4 Labelling 107.5 Refining the
27、 site-protocol 11Annex A (informative) Sampling protocols 12Bibliography . 14DIN EN 16150:2012-07 EN 16150:2012 (E) 3 Foreword This document (EN 16150:2012) has been prepared by Technical Committee CEN/TC 230 “Water analysis”, the secretariat of which is held by DIN. This European Standard shall be
28、given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by October 2012, and conflicting national standards shall be withdrawn at the latest by October 2012. Attention is drawn to the possibility that some of the elements of this document
29、may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austr
30、ia, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Ki
31、ngdom. DIN EN 16150:2012-07 EN 16150:2012 (E) 4 1 Scope This European Standard gives guidance on procedures for the pro-rata Multi-Habitat-Sampling (MHS) of benthic macro-invertebrates in wadeable rivers and streams. The term “pro-rata“ reflects the intention to sample adequate proportions of riveri
32、ne habitats with reference to their percentage occurrence. The pro-rata MHS technique does not replace other techniques, but is rather, alongside other applications, a fundamental requisite of some multi-metric assessment approaches used to evaluate the ecological status of running waters. The metho
33、d described in this document is one of the possible techniques among the existing pro-rata MHS techniques. The MHS methodology is based on Rapid Bioassessment Protocols 1, the procedures of the Environment Agency for England and Wales 2, the Austrian Guidelines for the Assessment of the Saprobiologi
34、cal Water Quality of Rivers and Streams 3, the AQEM sampling manual 4, the AQEM grain diameter 0,2 cm to 2 cm 3.2 argyllal silt, loam, clay 3.3 debris organic and inorganic matter deposited within the splash zone area by wave-motion and changing water levels 3.4 hygropetric sites thin water layer on
35、 solid (rocky) substrates 3.5 investigation site specific area of an investigated river reach for sampling benthic organisms DIN EN 16150:2012-07 EN 16150:2012 (E) 5 3.6 macro-algae strands of filamentous algae and algal tufts 3.7 macrolithal coarse blocks, cobbles, gravel and sand; grain diameter 2
36、0 cm to 40 cm 3.8 megalithal upper sizes of large cobbles, boulders, blocks and bedrock; grain diameter 40 cm 3.9 mesolithal fist to hand-sized cobbles with a variable percentage of gravel and sand; grain diameter 6 cm to 20 cm 3.10 micro-algae algal films 3.11 microlithal coarse gravel (size of a p
37、igeon egg to a childs fist) with variable percentages of medium to fine gravel; grain diameter 2 cm to 6 cm 3.12 pelal mud and sludge; grain diameter 40 cm large cobbles, bolders and blocks, bedrock Macrolithal 20 cm to 40 cm coarse blocks, head-sized cobbles (with variable percentages of cobbles, g
38、ravel and sand)Mesolithal 6 cm to 20 cm fist to hand-sized cobbles (with variable percentages of gravel and sand) Microlithal 2 cm to 6 cm coarse gravel (size of a pigeon egg to childs fist) (with variable percentages of medium to fine gravel) Akal 0,2 cm to 2 cm fine to medium-sized gravel Psammal
39、6 m to 2 mm sand Psammopelal mixture of sand with mud Pelal 40 cm macrolithal 20 cm to 40 cm mesolithal 6 cm to 20 cm microlithal 2 cm to 6 cm akal 0,2 cm to 2 cm psammal 6 m to 2 mm psammopelal pelal 6 m Argyllal 6 m not allocable % SU % SU % SU % SU % SU % SU % SU % SU % SU % SU % SU bare mineral
40、substrate BIOTICHABITATS- percent coverage(sumvariable)micro-algae macro-algae submerged macrophytes emergent macrophytes living parts of terrestial plants xyla CPOM FPOM debris sewage, bacteria and fungi 2) The user of this form is allowed to copy this present form. DI N E N 16150: 2012 - 07 EN 161
41、50:2012 (E) 14 Bibliography 1 BARBOUR, M.T., GERRITSEN, J., SNYDER, B.D. & STRIBLING, J.B. (1999): Rapid bioassessment protocols for use in streams and wadable rivers: periphyton, benthic macroinvertebrates and fish. (2nd Ed.) EPA 841-B-99-002. U.S. Environmental Protection Agency, Office of Water,
42、Washington, D.C. 2 MURRAY-BLIGH, J.A.D. (1999): Procedures for collecting and analysing macro-invertebrate samples. Quality Management Systems for Environmental Monitoring: Biological Techniques, BT001. (Version 2.0, 30 July 1999) - Environment Agency, Bristol, UK. 3 MOOG, O., CHOVANEC, A., HINTEREG
43、GER, J. & RMER, A. (1999): Austrian Guidelines for the assessment of the saprobiological water quality of rivers and streams (in German: Richtlinie zur Bestimmung der saprobiologischen Gewssergte von Fliegewssern).- Bundesministerium fr Land- und Forstwirtschaft. Wasserwirtschaftskataster, Wien, 144
44、 pp. 4 AQEM consortium (2002): Manual for the application of the AQEM system. A comprehensive method to assess European streams using benthic macroinvertebrates, developed for the purpose of the Water Framework Directive. Version 1.0. (www.aqem.de). 5 AQEM & STAR Site Protocol (2002): www.eu-star.at
45、. Protocols. 6 OENORM M 6119-2 (draft), Guidelines for the ecological study and assessment of rivers Macrozoobenthos. Part 2: A Standardized Procedure for prorate Multi-Habitat-Sampling. 7 OENORM M 6232, Guidelines for the ecological study and assessment of rivers (bilingual edition) 8 DIN 38410-1,
46、German standard methods for the examination of water, waste water and sludge Biological-ecological analysis of water (group M) Part 1: Determination of the saprobic index in running waters (M 1) 9 XP T90-333, Water quality Sampling of aquatic macro-invertebrates in shallow freshwaters DIN EN 16150:2012-07
