1、May 2013Translation by DIN-Sprachendienst.English price group 13No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 13.
2、080.30!%$/“2011259www.din.deDDIN EN ISO 11269-2Soil quality Determination of the effects of pollutants on soil flora Part 2: Effects of contaminated soil on the emergence and early growthof higher plants (ISO 11269-2:2012);English version EN ISO 11269-2:2013,English translation of DIN EN ISO 11269-2
3、:2013-05Bodenbeschaffenheit Bestimmung der Wirkungen von Schadstoffen auf die Bodenflora Teil 2: Wirkung von verunreinigten Bden auf Saatauflauf und frhes Wachstum hhererPflanzen (ISO 11269-2:2012);Englische Fassung EN ISO 11269-2:2013,Englische bersetzung von DIN EN ISO 11269-2:2013-05Qualit du sol
4、 Dtermination des effets des polluants sur la flore du sol Partie 2: Effets des sols contamins sur lmergence et la croissance des vgtauxsuprieurs (ISO 11269-2:2012);Version anglaise EN ISO 11269-2:2013,Traduction anglaise de DIN EN ISO 11269-2:2013-05SupersedesDIN ISO 11269-2:2006-09www.beuth.deIn c
5、ase of doubt, the German-language original shall be considered authoritative.Document comprises 27 pages04.13 DIN EN ISO 11269-2:2013-05 2 A comma is used as the decimal marker. National foreword The text of ISO 11269-2:2012 has been prepared by Technical Committee ISO/TC 190 “Soil quality” and has
6、been taken over as EN ISO 11269-2:2013 by Technical Committee CEN/TC 345 “Characterization of soils” (Secretariat: NEN, Netherlands). The responsible German body involved in its preparation was the Normenausschuss Wasserwesen (Water Practice Standards Committee), Working Committee NA 119-01-02-04 UA
7、 Biologische Verfahren of NA 119-01-02 AA Abfall- und Bodenuntersuchungen. ISO 11269 consists of the following parts, under the general title Soil quality Determination of the effects of pollutants on soil flora: Part 1: Method for the measurement of inhibition of root growth Part 2: Effects of cont
8、aminated soil on the emergence and early growth of higher plants Expert assistance and specialized laboratories will be required to perform the analysis described in this standard. DIN Standards corresponding to the International Standards referred to in this document are as follows: ISO 10381-6 DIN
9、 ISO 10381-6 ISO 10390 DIN ISO 10390 ISO 10694 DIN ISO 10694 ISO 11260 DIN EN ISO 11260 ISO 11268-1 DIN ISO 11268-1 ISO 11268-2 DIN ISO 11268-2 ISO 11274 DIN ISO 11274 ISO 11277 DIN ISO 11277 ISO 11465 DIN ISO 11465 ISO 22030 DIN EN ISO 22030 Amendments This standard differs from DIN ISO 11269-2:200
10、6-09 as follows: a) ISO 112692 has been taken over as European Standard EN ISO 11269-2; b) the method is now applicable to soils and contaminated soils; c) Annex A “Additional recommended plant species based on test results gained by applying Environment Canada Test Method: EPS 1/RM/45” (Biological
11、test method: Test for measuring Emergence and Growth of Terrestrial Plants Exposed to Contaminants in Soil) has been added; d) Annex B “Phytotoxic values for reference compounds: sodium trichloro-acetate and boric acid” has been supplemented; e) Annex D “Recommendations for nutrient supply of soils”
12、 has been added. Previous editions DIN ISO 11269-2: 1997-10, 2006-09 DIN EN ISO 11269-2:2013-05 3 National Annex NA (informative) Bibliography DIN ISO 11260, Soil quality Determination of effective cation exchange capacity and base saturation level using barium chloride solution DIN EN ISO 22030, So
13、il quality Biological methods Chronic toxicity in higher plants DIN ISO 10381-6, Soil quality Sampling Part 6: Guidance on the collection, handling and storage of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratory DIN ISO 10390, Soi
14、l quality Determination of pH DIN ISO 10694, Soil quality Determination of organic and total carbon after dry combustion (elementary analysis) DIN ISO 11268-1, Soil quality Effects of pollutants on earthworms (Eisenia fetida) Part 1: Determination of acute toxicity using artificial soil substrate DI
15、N ISO 11268-2, Soil quality Effects of pollutants on earthworms (Eisenia fetida) Part 2: Determination of effects on reproduction DIN ISO 11274, Soil quality Determination of the water retention characteristics Laboratory methods DIN ISO 11277, Soil quality Determination of particle size distributio
16、n in mineral soil material Method by sieving and sedimentation DIN ISO 11465, Soil quality Determination of dry matter and water content on a mass basis Gravimetric method DIN EN ISO 11269-2:2013-05 4 This page is intentionally blank EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 11269-2 F
17、ebruary 2013 ICS 13.080.30 English Version Soil quality - Determination of the effects of pollutants on soil flora - Part 2: Effects of contaminated soil on the emergence and early growth of higher plants (ISO 11269-2:2012) Qualit du sol - Dtermination des effets des polluants sur la flore du sol -
18、Partie 2: Effets des sols contamins sur lmergence et la croissance des vgtaux suprieurs Bodenbeschaffenheit - Bestimmung der Wirkungen von Schadstoffen auf die Bodenflora - Teil 2: Wirkung von verunreinigten Bden auf Saatauflauf und frhes Wachstum hherer Pflanzen (ISO 11269-2:2012) This European Sta
19、ndard was approved by CEN on 5 February 2013. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concernin
20、g such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into it
21、s own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germa
22、ny, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR
23、NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2013 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 11269-2:2013: E(ISO 11269-2:2012) Contents Page Foreword . 3 Introduction . 4 1 Scope . 5 2 Normative references.
24、 5 3 Terms and definitions 5 4 Units. 7 5 Principle 7 6 Test plants 7 7 Materials 8 7.1 Test vessels 8 7.2 Soil . 8 8 Equipment . 10 9 Reference substance . 10 10 Procedure 10 10.1 Experimental design 10 10.2 Preparation of the pots 11 10.3 Preparation of the seeds . 11 10.4 Growth conditions 12 10.
25、5 Start of the test . 12 10.6 Handling during the test 12 11 Validity criteria 13 12 Assessment of the results 13 12.1 Data presentation . 13 12.2 Expression of the results 13 13 Statistical analysis . 13 13.1 General 13 13.2 Range-finding test 14 13.3 Final test 14 14 Test report 11 Annex A (inform
26、ative) Additional recommended plant species based on test results gained by applying Environment Canada Test Method: EPS 1/RM/454. 17 Annex B (informative) Phytotoxic values for reference compounds: sodium trichloro-acetate and boric acid . 19 Annex C (informative) Recommended method for the measuri
27、ng of the water-holding capacity of the soil 20 Annex D (informative) Recommendations for nutrient supply of soils 21 Bibliography 22 2DIN EN ISO 11269-2:2013-05 EN ISO 11269-2:2013 (E) Foreword The text of ISO 11269-2:2012 has been prepared by Technical Committee ISO/TC 190 “Soil quality” of the In
28、ternational Organization for Standardization (ISO) and has been taken over as EN ISO 11269-2:2013 by Technical Committee CEN/TC 345 “Characterization of soils” the secretariat of which is held by NEN. This European Standard shall be given the status of a national standard, either by publication of a
29、n identical text or by endorsement, at the latest by August 2013, and conflicting national standards shall be withdrawn at the latest by August 2013. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be
30、held responsible for identifying any or all such patent rights. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Esto
31、nia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The
32、text of ISO 11269-2:2012 has been approved by CEN as EN ISO 11269-2:2013 without any modification. 3DIN EN ISO 11269-2:2013-05 EN ISO 11269-2:2013 (E) IntroductionThis part of ISO 11269 describes a procedure for evaluating the quality of soils of different origin carrying unknown contaminations. The
33、 evaluation of the effects on plant growth is based on emergence and inhibitory effects on early growth of at least two species of higher plants. Guidance for assessing potential effects of substances on seedling emergence and growth is given in OECD Guideline 20814.This part of ISO 11269 refers clo
34、sely to ISO 22030 and is based on:a) results from the German research project “Entwicklung eines innovativen und technischen Instrumentariums zur Optimierung der kotoxikologischen Bewertung von Bden im Hinblick auf Sanierungsziele und Schutzerfordernisse”;b) discussions within the joint project “Eco
35、toxicological Test Batteries” forming part of the BMBF Joint Research Group “Processes for the Bioremediation of Soil”23;c) results from the BMBF Joint Research Group ERNTE “Erprobung und Vorbereitung einer praktischen Nutzung kotoxikologischer Testsysteme”17;d) ring-test results of “Ecotoxicologica
36、l Characterisation of Waste Results and Experiences from an International Ring Test”8.Plant growth can be influenced strongly by soil properties such as texture, pH or levels of nutrients. When testing natural soils either reference soils (uncontaminated soils with the same properties as the test so
37、il) or standard soils are used as mixing and control substrate. In the latter case, variations in plant growth can result from either soil contaminants or differences in soil properties like nutrients and texture. Therefore, results from soil testing can less easily be interpreted than results from
38、testing of chemicals .4DIN EN ISO 11269-2:2013-05 EN ISO 11269-2:2013 (E) WARNING Contaminated soils may contain unknown mixtures of toxic, mutagenic, or otherwise harmful chemicals or infectious micro-organisms. Occupational health risks may arise from dust or evaporated chemicals during handling a
39、nd incubation. Furthermore, test plants might take up chemicals from the soil and safety measures should also be considered when handling the test plants.1 ScopeThis part of ISO 11269 describes a method to assess the quality of an unknown soil and the soil habitat function by determining the emergen
40、ce and early growth response of at least two terrestrial plant species compared to reference or standard control soils. It is applicable to soils of unknown quality, e.g. from contaminated sites, amended soils or soils after remediation.2 Normative referencesThe following referenced documents are in
41、dispensable for the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies.ISO 10381-6, Soil quality Sampling Part 6: Guidance on the collection, handling and storage
42、 of soil under aerobic conditions for the assessment of microbiological processes, biomass and diversity in the laboratoryISO 10390, Soil quality Determination of pHISO 10694, Soil quality Determination of organic and total carbon after dry combustion (elementary analysis)ISO 11260, Soil quality Det
43、ermination of effective cation exchange capacity and base saturation level using barium chloride solutionISO 11268-1, Soil quality Effects of pollutants on earthworms Part 1: Determination of acute toxicity to Eisenia fetida/Eisenia andreiISO 11268-2, Soil quality Effects of pollutants on earthworms
44、 Part 2: Determination of effects on reproduction to Eisenia fetida/Eisenia andreiISO 11277, Soil quality Determination of particle size distribution in mineral soil material Method by sieving and sedimentationISO 11465, Soil quality Determination of dry matter and water content on a mass basis Grav
45、imetric methodISO 22030, Soil quality Biological methods Chronic toxicity in higher plants3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.5DIN EN ISO 11269-2:2013-05 EN ISO 11269-2:2013 (E) 3.1emergenceappearance of the coleoptile or cotyledon abov
46、e the soil3.2contaminantsubstance or agent present in the soil as a result of human activityISO 15176:2002283.3hormesisimprovement of seedling emergence, growth or survival (or other response of the test plants) at low concentrations of chemicals or mixtures of soil that are toxic when applied at hi
47、gher levels in comparison to the control123.4lowest observed effect rate or effect concentrationLOEClowest tested percentage of a test soil in a reference or a standard control soil or concentration of a substance at which a statistically significant effect is observedNOTE The LOEC is expressed as a
48、 percentage of the test-soil dry mass per soil-mixture dry mass. All test mixtures above the LOEC have a harmful effect equal to or greater than that observed at the LOEC. If this condition cannot be satisfied, an explanation should be given for how the LOEC and NOEC (3.5) have been selected.3.5no o
49、bserved effect concentrationNOECtest-soil percentage immediately below the LOEC, which when compared to the control has no statistically significant effect (r 2) or ionizing substances into account, 5 % of peat have proven to be sufficient for maintaining the desired structure of the artificial soil. In this case, the respective percentages of the constituents are modified
