1、May 2017 English price group 10No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 67.200.10!%eB“2663197www.din.deDIN E
2、N ISO 663Animal and vegetable fats and oils Determination of insoluble impurities content (ISO 663:2017);English version EN ISO 663:2017,English translation of DIN EN ISO 663:2017-05Tierische und pflanzliche Fette und le Bestimmung des Anteils an unlslichen Verunreinigungen (ISO 663:2017);Englische
3、Fassung EN ISO 663:2017,Englische bersetzung von DIN EN ISO 663:2017-05Corps gras dorigines animale et vgtale Dtermination de la teneur en impurets insolubles (ISO 663:2017);Version anglaise EN ISO 663:2017,Traduction anglaise de DIN EN ISO 663:2017-05SupersedesDIN EN ISO 663:200903www.beuth.deDocum
4、ent comprises 14 pagesDTranslation by DIN-Sprachendienst.In case of doubt, the German-language original shall be considered authoritative.05.17 DIN EN ISO 663:2017-05 2 A comma is used as the decimal marker. National foreword This document (EN ISO 663:2017) has been prepared by Technical Committee I
5、SO/TC 34 “Food products” (Secretariat: AFNOR, France) in collaboration with Technical Committee CEN/TC 307 “Oil seeds, vegetable and animal fats and oils and their by-products Methods of sampling and analysis” (Secretariat: AFNOR, France). The responsible German bodies involved in its preparation we
6、re DIN-Normenausschuss Lebensmittel und landwirtschaftliche Produkte (DIN Standards Committee Food and Agricultural Products) and Deutsche Gesellschaft fr Fettwissenschaft (German Fat Research Society), NA 057-05-05 AA “Joint committee of DIN and DGF for the analysis of fats, oils and products there
7、of, related and primary products”. The DIN Standards corresponding to the International Standards referred to in this document are as follows: ISO 661 DIN EN ISO 661 ISO 5555 DIN EN ISO 5555 ISO 5725-1 DIN ISO 5725-1 ISO 5725-2 DIN ISO 5725-2 Amendments This standard differs from DIN EN ISO 663:2009
8、-03 as follows: a) milk and milk products (or fat coming from milk and milk products) are now excluded from the scope of the standard; b) the standard has been editorially revised and brought in line with the current rules of presentation. Previous editions DIN EN ISO 663: 1995-08, 2002-04, 2009-03
9、DIN EN ISO 663:2017-05 3 National Annex NA (informative) Bibliography DIN EN ISO 661, Animal and vegetable fats and oils Preparation of test sample DIN EN ISO 5555, Animal and vegetable fats and oils Sampling DIN ISO 5725-1, Accuracy (trueness and precision) of measurement methods and results Part 1
10、: General principles and definitions General principles and definitions DIN ISO 5725-2, Accuracy (trueness and precision) of measurement methods and results Part 2: Basic method for the determination of repeatability and reproducibility of a standard measurement method DIN EN ISO 663:2017-05 4 This
11、page is intentionally blank EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 663 February 2017 ICS 67.200.10 Supersedes EN ISO 663:2008English Version Animal and vegetable fats and oils - Determination of insoluble impurities content (ISO 663:2017) Corps gras dorigines animale et vgtale - Dt
12、ermination de la teneur en impurets insolubles (ISO 663:2017) Tierische und pflanzliche Fette und le - Bestimmung des Anteils an unlslichen Verunreinigungen (ISO 663:2017) This European Standard was approved by CEN on 8 February 2017. CEN members are bound to comply with the CEN/CENELEC Internal Reg
13、ulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN mem
14、ber. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions.
15、CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland
16、, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2017 CEN All rights of exploit
17、ation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 663:2017 EEuropean foreword .31 Scope . 52 Normative reference . 53 Terms and definitions . 54 Principle 55 Reagents 56 Apparatus . 67 Sampling 68 Preparation of test sample . 69 Procedure. 69.1 Test port
18、ion 69.2 Determination . 710 Expression of results 811 Precision . 811.1 Interlaboratory tests 811.2 Repeatability . 811.3 Reproducibility . 812 Test report . 8Annex A (informative) Results of interlaboratory tests 9Bibliography .10Contents PageDIN EN ISO 663:2017-05 EN ISO 663:2017 (E)2Foreword .4E
19、uropean foreword This document (EN ISO 663:2017) has been prepared by Technical Committee ISO/TC 34 “Food products” in collaboration with Technical Committee CEN/TC 307 “Oilseeds, vegetable and animal fats and oils and their by-products - Methods of sampling and analysis” the secretariat of which is
20、 held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by August 2017, and conflicting national standards shall be withdrawn at the latest by August 2017. Attention is drawn to the possibili
21、ty that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN ISO 663:2008. According to the CEN-CENELEC Internal Regulations, the national standards organ
22、izations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg,
23、Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 663:2017 has been approved by CEN as EN ISO 663:2017 without any modification. DIN EN ISO 663:2017-05 EN ISO 663:2017 (E)3
24、ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried out through ISO technical committees. Each member body interested in a subject for which a te
25、chnical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO collaborates closely with the International Electrotechnical Commission (IEC) on all matters
26、of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different approval criteria needed for the different types of ISO documents should be noted. This documen
27、t was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. ISO shall not be held responsible for identifying any or all su
28、ch patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www .iso .org/ patents).Any trade name used in this document is information given for the convenience of users and
29、does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the World Trade Organization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: ww
30、w .iso .org/ iso/ foreword .htmlThis document was prepared by Technical Committee ISO/TC 34, Food products, Subcommittee SC 11, Animal and vegetable fats and oils.This fifth edition cancels and replaces the fourth edition (ISO 663:2007), of which it constitutes a minor revision to exclude fat coming
31、 from milk and milk products.DIN EN ISO 663:2017-05 EN ISO 663:2017 (E)4 1 ScopeThis document specifies a method for the determination of the insoluble impurities content of animal and vegetable fats and oils.If it is not desired to include soaps (particularly calcium soaps) or oxidized fatty acids
32、in the insoluble impurities content, it is necessary to use a different solvent and procedure. In this case, an agreement is to be reached between the parties concerned.Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this document.2 Normative referen
33、ceThe following documents are referred to in the text in such a way that some or all of their content constitutes requirements of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) appl
34、ies.ISO 661, Animal and vegetable fats and oils Preparation of test sample3 Terms and definitionsFor the purposes of this document, the following terms and definitions apply.ISO and IEC maintain terminological databases for use in standardization at the following addresses: IEC Electropedia: availab
35、le at h t t p :/ www .electropedia .org/ ISO Online browsing platform: available at h t t p :/ www .iso .org/ obp3.1insoluble impurities contentquantity of dirt and other foreign matter insoluble in n-hexane or light petroleum under the conditions specified in this documentNote 1 to entry: The conte
36、nt is expressed as a percentage by mass.Note 2 to entry: These impurities include mechanical impurities, mineral substances, carbohydrates, nitrogenous substances, various resins, calcium soaps, oxidized fatty acids, fatty acid lactones, and (in part) alkali soaps, hydroxy-fatty acids and their glyc
37、erides.4 PrincipleA test portion is treated with an excess of n-hexane or light petroleum, then the solution obtained is filtered. The filter and residue are washed with the same solvent, then dried at 103 C and weighed.5 ReagentsWARNING Attention is drawn to the regulations which specify the handli
38、ng of dangerous substances. Technical, organizational and personal safety measures shall be followed.DIN EN ISO 663:2017-05 EN ISO 663:2017 (E)5 Use only reagents of recognized analytical grade.5.1 n-Hexane, or in the absence of this, light petroleum, having a distillation range between 30 C and 60
39、C and having a bromine value of less than 1.For either solvent, the residue on complete evaporation shall not exceed 0,002 g per 100 ml.5.2 Kieselgur, purified, calcinated, with loss in mass at 900 C (red heat) of less than 0,2 % by mass.6 ApparatusUsual laboratory apparatus and, in particular, the
40、following.6.1 Analytical balance, with an accuracy of 0,001 g.6.2 Electric drying oven, capable of operating at 103 C 2 C.6.3 Conical flask, of 250 ml capacity, with ground glass stopper.6.4 Desiccator, containing an efficient desiccant.6.5 Ashless filter paper (maximum ash content 0,01 %, by mass),
41、 retention value of 98 %, by mass, for particles of size greater than 2,5 m1), or an equivalent glass-fibre filter, of diameter 120 mm, together with a metal (preferably aluminium) or glass vessel with a well-fitting lid.These are alternatives to the filter (6.6) for all products except acid oils.6.
42、6 Filter crucible, glass, of grade P16 (pore size 10 m to 16 m), diameter 40 mm, of capacity 50 ml, together with a suction bottle.This is an alternative to 6.5 for all products including acid oils.7 SamplingA representative sample should have been sent to the laboratory. It should not have been dam
43、aged or changed during transport or storage.Sampling is not part of the method specified in this document. A recommended sampling method is given in ISO 5555.8 Preparation of test samplePrepare the test sample in accordance with ISO 661.9 Procedure9.1 Test portionWeigh, to the nearest 0,01 g, approx
44、imately 20 g of the test sample (Clause 8) into a conical flask (6.3).1) Whatman 42 (2,5 m) filter paper or Whatman GF/D glass-fibre filter are examples of suitable products available commercially. This information is given for the convenience of users of this document and does not constitute an end
45、orsement by ISO of these products.DIN EN ISO 663:2017-05 EN ISO 663:2017 (E)6 9.2 Determination9.2.1 Dry the filter paper and the vessel (6.5) with its lid, or the filter crucible (6.6), in the oven (6.2) set at 103 C. Allow to cool in the desiccator (6.4) then weigh to the nearest 0,001 g. For acid
46、 oils, prepare the crucible as described in 9.2.7 and continue as in 9.2.2.9.2.2 Add 200 ml of n-hexane or light petroleum (5.1) to the flask containing the test portion (9.1). Stopper the flask and shake.For castor oil, the quantity of solvent may be increased to facilitate the operation and this m
47、ay necessitate the use of a larger flask.Leave to stand at about 20 C for about 30 min.9.2.3 Filter through the filter paper in a suitable funnel or through the filter crucible, using suction if necessary. Rinse the flask to ensure that all impurities are washed into the filter/crucible.Wash the fil
48、ter paper or filter crucible by pouring through it small amounts of the same solvent as used in 9.2.2, but no more than is necessary for the final filtrate to be free from fat or oil. Warm the solvent, if necessary, to a maximum temperature of 60 C in order to dissolve any solidified fats retained on the filter.9.2.4 If a filter paper is used, remove it from the funnel and place it in the vessel. Allow most of the solvent remai
