1、September 2007DEUTSCHE NORM English price group 13No part of this standard may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 13.060.70!$I)“138065
2、7www.din.deDDIN EN ISO 19493Water quality Guidance on marine biological surveys of hard-substrate communities(ISO 19493:2007)English version of DIN EN ISO 19493:2007-09Wasserbeschaffenheit Anleitung fr meeresbiologische Untersuchungen von Hartsubstratgemeinschaften(ISO 19493:2007)Englische Fassung D
3、IN EN ISO 19493:2007-09www.beuth.deDocument comprises 29 pages 11.07DIN EN ISO 19493:2007-09 2 This standard is part of the series Deutsche Einheitsverfahren zur Wasser-, Abwasser- und Schlammunter-suchung Biologisch-kologische Gewsseruntersuchung (Gruppe M) (German standard methods for the examinat
4、ion of water, waste water and sludge Biological-ecological methods of analysis (group M) and describes method M 51. National foreword This standard has been prepared by Technical Committee CEN/TC 230 “Water analysis” (Secretariat: DIN, Germany) in collaboration with Technical Committee ISO/TC 147 “W
5、ater quality”. The responsible German body involved in its preparation was the Normenausschuss Wasserwesen (Water Practice Standards Committee), Technical Committees NA 119-01-03-05-06 AK Biologisch-kologische Gewsseruntersuchung and NA 119-01-03 AA Wasseruntersuchung. This standard has been publish
6、ed to implement the Water Framework Directive (WFD), Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy)1). Expert assistance and specialized laboratories will be required to perform the ana
7、lysis described in this standard. Existing safety requirements are to be observed. Depending on the objective of the analysis, a check shall be made on a case-by-case basis as to whether and to what extent additional conditions will have to be specified. Designation of method M 51 Guidance on marine
8、 biological surveys of hard-substrate communities: Method DIN EN ISO 19493 M 51 Standard methods published as DIN Standards are obtainable from Beuth Verlag GmbH, either individually or grouped in volumes. The standard methods included in the loose-leaf publication entitled Deutsche Einheitsverfahre
9、n zur Wasser-, Abwasser- und Schlammuntersuchung will continue to be published by Wiley-VCH Verlag and Beuth Verlag GmbH. All standard methods relevant to the Abwasserverordnung (Waste Water Regulation) (AbwV) included in the new Regulation on Section 7a of the Gesetz zur Ordnung des Wasserhaushalts
10、 (German Water Management Act) concerning Anforderungen an das Einleiten von Abwasser in Gewsser together with the Abwasser-verordnung and the Gesetz zur Ordnung des Wasserhaushalts have been published by Beuth-Verlag GmbH as a loose-leaf collection Analysenverfahren in der Abwasserverordnung Rechts
11、vorschriften und Normen (Supplements). Standards or draft standards bearing the group title “German standard methods for the examination of water, waste water and sludge” are classified under the following categories (main titles): General information (group A) (DIN 38402) Sensory analysis (group B)
12、 (DIN 38403) Physical and physicochemical parameters (group C) (DIN 38404) Anions (group D) (DIN 38405) Cations (group E) (DIN 38406) Substance group analysis (group F) (DIN 38407) Gaseous constituents (group G) (DIN 38408) Parameters characterizing effects and substances (group H) (DIN 38409) 1)Reg
13、istered in the DITR database of DIN Software GmbH, available from: Beuth Verlag GmbH, 10772 Berlin, Germany. DIN EN ISO 19493:2007-09 3 Biological-ecological methods of analysis (group M) (DIN 38410) Microbiological methods (group K) (DIN 38411) Test methods using water organisms (group L) (DIN 3841
14、2) Individual constituents (group P) (DIN 38413) Sludge and sediments (group S) (DIN 38414) Bio-assays with microorganisms (group T) (DIN 38415) In addition to the methods described in the DIN 38402 to DIN 38415 series of standards, there are a number of European and International Standards availabl
15、e as DIN EN, DIN EN ISO and DIN ISO Standards which also form part of the collection of German standard methods. Information on Parts of these series of standards that have already been published can be obtained from the offices of the Normenausschuss Wasserwesen, telephone +49 (30) 2601-2448, or fr
16、om Beuth Verlag GmbH, Burggrafenstrae 6, 10787 Berlin, Germany. The DIN Standards corresponding to the International Standards referred to in clause 2 of the EN are as follows: ISO 5667-1 DIN EN ISO 5667-1 ISO 5667-3 DIN EN ISO 5667-3 ISO 5667-19 DIN EN ISO 5667-19 ISO 8601 DIN ISO 8601 ISO 16665 DI
17、N EN ISO 16665 National Annex NA (informative) Bibliography DIN EN ISO 5667-1, Water quality Sampling Part 1: Guidance on the design of sampling programmes and sampling techniques DIN EN ISO 5667-3, Water quality Sampling Part 3: Guidance on the preservation and handling of water samples DIN EN ISO
18、5667-19, Water quality Sampling Part 19: Guidance on sampling of marine sediments DIN EN ISO 16665, Water quality Guidelines for quantitative sampling and sample processing of marine soft-bottom macrofauna DIN ISO 8601, Data elements and interchange formats Information interchange Representation of
19、dates and times DIN EN ISO 19493:2007-09 4 This page is intentionally blank EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 19493 June 2007 ICS 13.060.10; 13.060.70 English Version Water quality - Guidance on marine biological surveys of hard-substrate communities (ISO 19493:2007) Qualit de
20、 leau - Lignes directrices pour les tudes biologiques marines des peuplements du substrat dur (ISO 19493:2007) Wasserbeschaffenheit - Anleitung fr meeresbiologische Untersuchungen von Hartsubstratgemeinschaften (ISO 19493:2007) This European Standard was approved by CEN on 9 June 2007. CEN members a
21、re bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to
22、 the CEN Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Management Centre has the sa
23、me status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
24、Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: rue de Stassart, 36 B-1050 Brussels 2007 CEN All rights of exploitation in any form and by any means res
25、erved worldwide for CEN national Members. Ref. No. EN ISO 19493:2007: EContents Page Foreword Introduction 1 Scope . 2 Terms and definitions. 3 Quality and safety. 4 Strategies and objectives for hard-substrate surveys 5 6 Taxon identification and sample processing. Annex B (informative) Field recor
26、ding form Annex C (informative) Biological definition of the supralittoral, eulittoral and sublittoral zones . Annex D (informative) Basis for the choice of semi-quantitative surveys in a standard . Annex E (informative) Detailed method for calculating level of exposure . Bibliography . 2 EN ISO 194
27、93:2007 (E) 45 5781721 22 2324 25. 315 12SamplingAnnex A (normative) Description of methods. Foreword This document (EN ISO 19493:2007) has been prepared by Technical Committee CEN/TC 230 “Water analysis”, the secretariat of which is held by DIN, in collaboration with Technical Committee ISO/TC 147
28、“Water quality”. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2007, and conflicting national standards shall be withdrawn at the latest by December 2007. According to the CEN/CENELEC I
29、nternal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg,
30、 Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. 3 EN ISO 19493:2007 (E) Introduction Surveys of benthic marine algae and fauna on hard substrates represent an important part of marine environmental surveys. The species compos
31、ition, both in terms of the species present and their relative abundances, is a result of the natural and anthropogenic environmental factors at the survey site. Natural factors that influence species composition include wave exposure, depth, salinity, nutrient level, type of substrate, slope, orien
32、tation, turbidity, current, temperature and grazing. Anthropogenic factors include pollution (e.g. oil, contaminants, particles), physical disturbance, elevated nutrient levels and effects from fisheries. A number of different methods are being used to investigate flora and fauna on hard substrate a
33、ccording to the survey aim and the type of biotope surveyed. To allow environmental authorities and others to make use of this knowledge, it is essential that surveys are intercomparable in time and space, as well as between operators, and that the data are of a high quality. This International Stan
34、dard is based on a limited selection of methods that allow precise documentation, that are replicable and which have been tested over many years. In choosing methods for this standard, semi-quantitative and quantitative techniques have been emphasized, such that species and quantities can be related
35、 to a known area of sea floor. For the purposes of this International Standard, hard substrate is defined as bedrock, stable rocks and fixed marine constructions (e.g. pipelines and quays). The main focus is on biological surveys based on species that can be recorded in the field (i.e. that are visi
36、ble to the naked eye). The guidelines are applicable to seagrass communities and their epiflora and epifauna. They can also be used for surveys of stable substrates comprising loose pebbles/boulders, stone blocks, coarse gravel and other loose material as well as bedrock covered with loose sediment,
37、 but in general, such substrates require specially adapted techniques. Additional methods are usually required for surveys in depths greater than approximately 30 m. For sediment sampling in marine areas, refer to ISO 5667-19. For surveys of sublittoral soft-bottom fauna, see ISO 16665. 4 EN ISO 194
38、93:2007 (E) 1 Scope This International Standard provides guidance for marine biological surveys of supralittoral, eulittoral and sublittoral hard substrate for environmental impact assessment and monitoring in coastal areas. This International Standard comprises development of the sampling programme
39、, survey methods, species identification, and storage of data and collected material. This International Standard specifies the minimum requirements for environmental monitoring. The methods are limited to surveys and semi-quantitative and quantitative recording techniques that cause little destruct
40、ion of the fauna and flora. In practice, this refers to direct recording in the field and photography. Sampling by scraping off organisms, use of a suction sampler, etc. are not covered in this International Standard, but such techniques can be used as a supplement to obtain information on small-siz
41、ed species or those that live hidden. 2 Terms and definitions For the purposes of this document, the following terms and definitions apply. 2.1 area of influence area influenced or expected to be influenced, based on the available information 2.2 biotope area of uniform environmental conditions (hab
42、itat) and its characteristic assemblage of plant and/or animal species EXAMPLE Laminaria hyperborea community (cuvie or tangleweed), knotted wrack community, blue mussel belt. 2.3 macroscopic organisms algae and animals that are visible without magnification equipment (W 1 mm) and which can be recor
43、ded in the field NOTE Certain macroscopic organisms can require microscopic inspection for identification. For microscopy of collected material, the lower size limit is set to 1 mm. 5 EN ISO 19493:2007 (E) 2.4 hard substrate substrate consisting of bedrock, larger rocks/stones or fixed marine constr
44、uctions such as wharfs, quays and pipelines NOTE For the purpose of this International Standard, hard substrate can also include other substrates that are not likely to be moved or turned over during a reasonable time period so that perennial species communities are likely to develop (e.g. pebbles a
45、nd stones in sheltered environments). 2.5 hard substrate flora and fauna attached algae and animals, together with relatively stationary animals living on or in close association with hard substrate EXAMPLE Attached: kelp, seaweeds, sponges, bryozoans, corals, mussels, barnacles, ascidians. Relative
46、ly stationary: snails, sea-urchins, crabs. 2.6 supralittoral zone zone above the eulittoral zone, which is reached by spray water NOTE Its upper limit is normally determined by the upper limit of Verrucaria (black lichen belt), blue-green algae (usually Calothrix scopulorum) or littorinid snails. 2.
47、7 eulittoral zone marine intertidal zone which is submersed and emerged, either periodically due to tides or aperiodically due to irregularly occurring factors, as in the enclosed seas of the Baltic or the Mediterranean NOTE Biologically, this zone is defined as the zone between the upper limit of b
48、arnacles and the upper limit of laminarians. In the Baltic where there is no tide, the eulittoral zone is the zone of short-lived annual algae. 2.8 sublittoral zone zone below the eulittoral zone, which is submersed with the upper part at extreme low water levels occasionally emerging NOTE 1 In this
49、 International Standard, the lower limit is set by the deepest occurring algae. NOTE 2 This is also referred to as the subtidal zone. NOTE 3 Biologically, this zone is defined as the zone between the upper limit of laminarians and the lower limit of algal vegetation (see Annex C). 2.9 level of exposure level of wave and current exposure at a site 2.10 receiving water body water body which receives an input of material, of either natural or anthropogenic origin NOTE The term often appears in the context of contamination (for
