1、 STD-BSI BS EN 1377-ENGL 1777 M Lb24bb7 Ob3320b 171 BRITISH STANDARD Foodstuffs - Determination of acesulfame K in table top sweetener preparations - Spectrometric method The European Standard EN 1377 : 1996 has the status of a British Standard ICs 67.180.10 NO COPYING WITHOUT BSI PERMISSION EXCEPT
2、AS PERMITTED BY COPYRIGHT LAW BS EN 1377 : 1997 STD*BSI BS EN L377-ENGL 1977 U 1b2ibbS Ob33207 008 BS EN 1377 : 1997 Committees responsible for this British Standard The preparation of this BriW Standard was entrusted to Technicai Panel AW/-B, Food analysis - Horizontal methods, upon which the follo
3、wing bodies were represented kcsociation of Public Analysts Food and Drink Federation Institute of Food Science and Technology Laboratory of the Government Chemist Muistry of Agricuiture, Fisheries and Food Royal Society of Chemistry This British Standard, having been prepared under the direction of
4、 the Consumer Products and Services Sector Board, was published under the authority of the Standards Board and comes into effect on 15 May 1997 O BSI 1997 Amendments issued since publication Amd. No. Date I Te*affectd The following BSI references relate to the work on this standard Conunittee refere
5、nce AWJ-J3 Draft for comment 94501925 DC ISBN O 580 27123 4 BS EN 1377 : 1997 Contents Page Committees responsible Inside front cover Nationai foreword ii Foreword 2 Text of EN 1377 3 O BSI 1997 i STD-BSI BS EN 1377-ENGL 1777 D Lb2Libb7 Ob33207 780 BS EN 1377 : 1997 National foreword This British St
6、andard has been prepared by Technid Panel AWM and is the English ianguage version of EN 1377 : 1996 Foodstuffs - Determinution of acesulfame K in table top sweetenerpepamtions - Spectwmetric method, published by the European Committee for Standardizaton (CEN). EN 1377 was produced as a result of int
7、ernational discussions in which the United Kingdom took an active part. Cross-references Publication referred to EN IS0 3696 : 1995 Corresponding British Standard BS EN Is0 36% : 1995 Water for analyticd Zuomtorg use. Spedfatwn and test methods IS0 5725 : 1986, to which infornative reference is made
8、 in the text, has been superseded by IS0 57251 : 1994, IS0 57252 : 1994, IS0 5725-3 : 1994, IS0 5725-4 : 1994 and IS0 5725-6 : 1994 which are identical with Bs IS0 5725 Accu- (trueness and precision) of measurement methods and reJults, BS IS0 57251 : 1994 General prim3pk.s and definitions, BS IS0 57
9、252 : 1994 Basic method for the determination of wpeutability and rt.rprodUcibiLity of a standarol measurement method, BS IS0 57253 : 1994 IntemLea.iate measures of thepreciswn of a standard mec2su-t method, BS IS0 57254 : 1994 Basic method for the determinacion of th Crueness of a standard rneasure
10、naent method, and BS IS0 572543 : 1994 Use in practice of accumcy values. Compliance with a British Standard does not of itself confer immunity from legal obligations. Summary of pages This document comprises a front cover, an inside front cover, pages i and ii, the EN title page, pages 2 to 6, an i
11、nside back cover and a back cover. ii O BSI 1997 STD-BSI BS EN 1377-ENGL 1977 Lb24bb9 Ob33210 bT2 - EUROPEAN STANDARD EN 1377 NORME EUROPENNE EUROP- NORM September 1996 ICs 67.180.10 Descriptors: Focid products, intense sweeteners, chemical anaiysis, determination of content, spectrometric anaiysis
12、Foodst Al is the absorption of the acesulfame K standard solution (6.3.1); is the initiai sample mass (6.2) in milligrams; ml is the mass of the acesulfame K standard substance in 500 ml stanard solution (4.3) in milligrams (here: 4,O mg); is the ilution factor (here: 100). F 7.2 Caiculate the mass
13、I?action, w2 of acesulfame K, in milligrams per tablet, using the following equation: where: mz is the average tablet mass (6.1)7 in milligrams; mo see quation (i)* if the calculation is based on a dibraiion graph, an alternative calculative evaiuation using the regression graph may be used Report t
14、he result afim rounding to one decimai place. 1997 lb24bb b33213 301 M 8 Precision Details of the inter-laboratory test of the precision of the method according to IS0 5725 : 19% 3 are summarized in annex B. The values derived from the inter-laboratory test may not be applicable to adyte concentrati
15、on ranges and matrices other than given in annex B. 8.1 Repeatability The absolute ifference between two single test results found on identical test material by one operator using the same apparatus within the shortest feasible time interval will exceed the repeatability limit T in not more than 5 %
16、 of the cases. The value is: T = 3,2 mg100 mg for commercially available acesuihne K tablets. 8.2 Reproducibility The absolute Merence between two single test results on identical test material reported by two laboratones will exceed the reproducibility limit R in not more than 5 % of the cases. The
17、 value is: R = 3,7 mg/100 mg for commercially available acesulfame K tablets. 9 Wst report The test report shall contain at least the following data: - ail infomation necessary for the identication of the sample; - a reference to this Euro- Standard or to the method used; -the results and the units
18、in which the results have been expressed; - if the repeatability of the method has been verified; - any particular points observed in the com of the test; - any operations not specified in the method or regarded as optionai which might have afected the results. O BSI 1997 STD-BSI BS EN 1377-ENGL 277
19、7 Lb24bbS Ob33219 248 Page 5 EN 1377 : 1996 1 e O n L O v) D U Annex A (informative) Figure Sample: Acesulfame K Solvent: Water Concentration: 8,O mg/i Function: Absorption Wavelength range: 2OOnm to3OOnm Wavelength - Recognized maximum: 1: Wavelength = 228 nm Figure A.l. Absorption spectrum of an a
20、cesulfame K standard solution in water Absorption = 0,484333 Page 6 EN 1377 : 1996 Mean value X Repeatability standard deviation s, ReDeatabiliW relative standard deviation RSD, Annex B (informative) Precision data In accordance with Is0 5725 : 1986 3, the following parameters have been defined in a
21、n inter-laboratory test The test was conducted by the Max von Petknkofer Institute of the Federal Heaith Office, Food Chemistry Department, Berlin, Germany i. 98,O mgIl00 mg 1,l mgIl00 mg 1,2 % Table B.l. Reproducibility standard deviation SR Reproducibility relative standard deviation RSDR Reproduc
22、ibility limit R Sample Year of inter-labomry test Number of laboratories Number of samples Number of laboratories retained affer ehhating outliers Number of outliers (aboratories) Number of accepted results 1,3 mgIl00 mg 1,3 % 3,7 mg/100 mg Commercially available acesulfame tablets 1986 8 1 7 1 38 R
23、epeatability limit r I 3,2 mg/100 mg Annex C (Wormative) Bibliography i Untersuchung wn Lebensmitteln: Bestimmung des AcesUfam-K-Gehaltes in acesulfam-K-haltigen Sgstofl-TaOletten L 57.22.M, Mai 1989 (Food Analysis: Detemzimtiun of acicial methods under article 35 of #e Gmn Federal Foods Act; Method
24、s of sampling and adysis of foods, tobacco pducts, cosmetics und commodity goods, Federal Heaith Office) Loseblattausgabe, Stand Mai 1994. Bd. 1. (Looseleaf edition of 1994-05. Vol. I.) Berlin, Kln: Beuth Verlag GmbH. 2 FA0 Food and Nutrition Paper 28 of the Joint FAO/”I-IO Expert Committee on Food
25、Additives, April 1980. 3 IS0 5725 : 1986Frecisiun of test meethods - Detemzimtiort of repeutubity and vducibity for a standard test method by in.!#-laboratoyy tests. O BSI 1997 STD.BS1 BS EN 1377-ENGL 1777 Lb24bb7 Ub332Lb O10 BS EN 1377 : 1997 List of references See national foreword. O BSI 1997 BS
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