1、BS EN 46-2:2009ICS 71.100.50NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBRITISH STANDARDWood preservatives Determinationof the preventiveaction against recentlyhatched larvae ofHylotrupes bajulus(Linnaeus)Part 2: Ovicidal effect (laboratorymethod)Copyright European Committe
2、e for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-This British Standardwas published under theauthority of the StandardsPolicy and StrategyCommittee on 31 December2009 BSI 2009ISBN 978 0 580 66780 0Amendment
3、s/corrigenda issued since publicationDate CommentsBS EN 46-2:2009National forewordThis British Standard is the UK implementation of EN 46-2:2009. Itsupersedes BS EN 46-2:2006 which is withdrawn.The UK participation in its preparation was entrusted to TechnicalCommittee B/515, Wood preservation.A lis
4、t of organizations represented on this committee can be obtained onrequest to its secretary.This publication does not purport to include all the necessary provisionsof a contract. Users are responsible for its correct application.Compliance with a British Standard cannot confer immunityfrom legal ob
5、ligations.Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN 46-2:2009EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 46-2 November 2009 ICS 71.100.50 Supersedes EN 46-2:
6、2006English Version Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 2: Ovicidal effect (laboratory method) Produits de prservation du bois - Dtermination de laction prventive contre les larves rcemment closes dHylotr
7、upes bajulus (Linnaeus) - Partie 2: Effet ovicide (Mthode de laboratoire) Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenber frisch geschlpften Larven von Hylotrupes bajulus (Linnaeus) - Teil 2: Ovidize Wirkung (Laboratoriumverfahren) This European Standard was approved by CEN on 10 Oct
8、ober 2009. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be ob
9、tained on application to the CEN Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN Mana
10、gement Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, No
11、rway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: Avenue Marnix 17, B-1000 Brussels 2009 CEN All rights of exploitation in any for
12、m and by any means reserved worldwide for CEN national Members. Ref. No. EN 46-2:2009: ECopyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN 46-2:2009EN 46-2:2009 (E) 2 Contents
13、 Page Foreword 4Introduction . 51 Scope 62 Normative references 63 Terms and definitions . 64 Principle 75 Test materials . 75.1 Biological material . 75.2 Products and reagents 75.3 Apparatus . 76 Sampling . 87 Test specimens 87.1 Species of wood 87.2 Wood quality 87.3 Provision of test specimens .
14、 97.4 Dimensions of test specimens . 97.5 Number of test specimens 97.5.1 Test specimens for egg-laying . 97.5.2 Test specimens for checking the tunnelling ability and the mortality of the larvae 98 Procedure . 108.1 Preparation of the test specimens . 108.1.1 Conditioning of the test specimens prio
15、r to sealing . 108.1.2 Sealing of block faces . 108.1.3 Treatment of the test specimens . 108.1.4 Drying and conditioning of the test specimens after treatment . 128.2 Exposure of the test specimens to the insects (ovicidal action) . 128.3 Validity of the test 139 Expression of results 139.1 Ovicida
16、l test . 139.2 Tunnelling control . 139.3 Total mortality 1310 Test report 14Annex A (informative) Example of a test report . 16Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus) . 19B.1 General 19B.2 Obtaining parent beetles. 19B.3 Mating . 19B.4 Egg-Iaying 19B.5 Hatching o
17、f eggs 20B.6 Larval development . 20B.7 Enemies and parasites 21Annex C (informative) Differentiation of heartwood and sapwood in Pinus species 22C.1 Principle 22C.2 Reagents . 22Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or n
18、etworking permitted without license from IHS-,-,-BS EN 46-2:2009EN 46-2:2009 (E) 3 C.3 Apparatus . 22C.4 Procedure . 22Annex D (informative) Environmental, health and safety precautions within chemical/biological laboratory . 23Bibliography 24Copyright European Committee for Standardization Provided
19、 by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN 46-2:2009EN 46-2:2009 (E) 4 Foreword This document (EN 46-2:2009) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and wood-based products”, the secretariat of
20、 which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by May 2010, and conflicting national standards shall be withdrawn at the latest by May 2010. Attention is drawn to the possib
21、ility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document supersedes EN 46-2:2006. The standard EN 46 is composed of two parts: EN 46-1, Wood preservatives Determ
22、ination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 1: Application by surface treatment (laboratory method) EN 46-2, Wood preservatives Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 2: O
23、vicidal effect (laboratory method) EN 46 consists of two parts to enable preventive action of wood preservatives, against recently hatched larvae of Hylotrupes bajulus, which are intended to be applied by surface treatment; Part 1 is required to determine the larvicidal effect of preservatives and P
24、art 2 is required to determine the ovicidal action of the preservatives after egg-laying of young females. Significant technical differences between this document and EN 46-2:2006 are as follows: a) For treatment by dipping sealing of all faces except the one which is exposed to the insects added; b
25、) Requirements for the selection of test specimens specified in more detail in 7.5.1. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Republic,
26、Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. Copyright European Committee for Standardization Provided
27、 by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN 46-2:2009EN 46-2:2009 (E) 5 Introduction This test method describes a laboratory method of test which gives a basis for the assessment of the preventive action of a wood preservativ
28、e, when applied as a surface treatment for timber, against eggs of Hylotrupes bajulus. In combination with EN 46-1 it provides a means of checking whether larvae may hatch from eggs laid on the treated wood surface and whether they are capable of boring through the treated surface and of surviving i
29、n the untreated part of the wood. This amended standard provides for the sealing of all but one lateral face when specimens are to be treated by dipping. When products which are very active at very low concentration are used, it is very important to take suitable precautions to isolate and separate,
30、 as far as possible, operations involving chemical products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel (see also A
31、nnex D for environmental, health and safety precautions). Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN 46-2:2009EN 46-2:2009 (E) 6 1 Scope This European Standard specifi
32、es a method for the determination of the preventive action of a wood preservative against eggs of Hylotrupes bajulus (Linnaeus) when the preservative is applied as a surface treatment to wood. This method is applicable to: water-insoluble chemicals which are being studied as active insecticides; org
33、anic formulations, as supplied or as prepared in the laboratory by dilution of concentrates; organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; or water-soluble materials, for example salts. The method is applicable whether or not the tes
34、t specimens have been subjected to appropriate ageing procedures. 2 Normative references The following referenced documents are indispensable for the application of this document. For dated references, only the edition cited applies. For undated references the latest edition of the referenced docume
35、nt (including any amendments) applies. EN 46-1, Wood preservatives Determination of the preventive action against Hylotrupes bajulus (Linnaeus) Part 1: Larvicidal effect (laboratory method) EN 73, Wood preservatives Accelerated ageing tests of treated wood prior to biological testing Evaporative age
36、ing procedure EN 84, Wood preservatives Accelerated ageing of treated wood prior to biological testing Leaching procedure EN ISO 3696:1995, Water for analytical laboratory use Specification and test methods (ISO 3696:1987) 3 Terms and definitions For the purposes of this document, the following term
37、s and definitions apply. 3.1 representative sample sample having its physical and/or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled EN 1001-2:2005, 4.71 3.2 supplier sponsor of the test (person or company providing the sample of wood pr
38、eservative to be tested) NOTE Adapted from EN 1001-2:2005, 4.83. Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN 46-2:2009EN 46-2:2009 (E) 7 4 Principle In this laboratory
39、method treated wood panels are offered to freshly mated Hylotrupes bajulus females. The hatching ability of the larvae on the treated timber is examined. When the ovicidal action is insufficient, the mortality of the hatched larvae on and/or in wood treated with the same formulation is also establis
40、hed according to EN 46-1. 5 Test materials 5.1 Biological material 5.1.1 Hylotrupes bajulus (Linnaeus) females. 5.1.2 Source of females The insects shall preferably be obtained from cultures reared as e.g. described in Annex B. Use only sound and lively insects. 5.2 Products and reagents 5.2.1 Paraf
41、fin wax, for fixing the glass plate in all cases and for sealing the end faces of test specimens to be treated with solutions in all cases in which water is the continuous phase. NOTE Paraffin wax with a setting point of 52 C to 54 C has been found to be suitable. 5.2.2 Gelatine, for sealing the end
42、 faces of test specimens to be treated with solutions in which an organic solvent is the continuous phase. 5.2.3 Water, complying with grade 3 of EN ISO 3696:1995. 5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at th
43、e end of the post-treatment conditioning period that has a toxic effect on the insects. CAUTION Do not use benzene or other solvents which pose a health risk. 5.3 Apparatus 5.3.1 Culturing chamber, with air circulation, and controlled at (28 2) C and at relative humidity (70 5) %. 5.3.2 Conditioning
44、 chamber, well ventilated and controlled at (20 2) C and at relative humidity (65 5) %. NOTE The conditioning of test specimens may be carried out in the laboratory work area (see 5.3.3) provided that this has the conditions specified for the conditioning chamber (see 5.3.2). 5.3.3 Laboratory work a
45、rea, well ventilated, where treatment of the test specimens is carried out. CAUTION It is essential to follow safety procedures for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours. 5.3.4 Testing chamber, ventilated and air-conditioned, contr
46、olled at (22 2) C and at relative humidity between (70 5) %. Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN 46-2:2009EN 46-2:2009 (E) 8 5.3.5 Petri dishes of glass or poly
47、vinylchloride (PVC), diameter ca. 9 cm for mating the insects and for egg-laying. 5.3.6 Weights, to provide ballast for the test specimens. The weights shall not react with any materials with which they come into contact during the test. 5.3.7 Safety equipment, protective clothing, appropriate for t
48、he test product and the test solvent, to ensure the safety of the operator. 5.3.8 Glass plates, 48 mm long and 25 mm wide, intended to provide a lateral slit on the test specimens. 5.3.9 Ordinary laboratory equipment, including a balance capable of weighing to an accuracy of 0,01 g and equipment for applying a liquid product by brushing or by pipette. 5.3.10 Protective gloves 6 S
