1、Microbiology of the food chain Horizontal method for detection and enumeration of Campylobacter spp. Part 1: Detection methodBS EN ISO 10272-1:2017BSI Standards PublicationWB11885_BSI_StandardCovs_2013_AW.indd 1 15/05/2013 15:06EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 10272-1 July 20
2、17 ICS 07.100.30 Supersedes EN ISO 10272-1:2006English Version Microbiology of the food chain - Horizontal method for detection and enumeration of Campylobacter spp. - Part 1: Detection method (ISO 10272-1:2017) Microbiologie de la chane alimentaire - Mthode horizontale pour la recherche et le dnomb
3、rement de Campylobacter spp. - Partie 1 : Mthode de recherche (ISO 10272-1:2017) Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zum Nachweis und zur Zhlung von Campylobacter spp. - Teil 1: Nachweisverfahren (ISO 10272-1:2017) This European Standard was approved by CEN on 1 May 2017. CE
4、N members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on app
5、lication to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC M
6、anagement Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, La
7、tvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: A
8、venue Marnix 17, B-1000 Brussels 2017 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 10272-1:2017 ENational forewordThis British Standard is the UK implementation of EN ISO 10272-1:2017. It is identical to ISO 10272-1:2017. It
9、 supersedes BS EN ISO 10272-1:2006, which is withdrawn.The UK participation in its preparation was entrusted to Technical Committee AW/9, Microbiology.A list of organizations represented on this committee can be obtained on request to its secretary.This publication does not purport to include all th
10、e necessary provisions of a contract. Users are responsible for its correct application. The British Standards Institution 2017 Published by BSI Standards Limited 2017ISBN 978 0 580 82700 6ICS 07.100.30Compliance with a British Standard cannot confer immunity from legal obligations.This British Stan
11、dard was published under the authority of the Standards Policy and Strategy Committee on 31 August 2017.Amendments/corrigenda issued since publicationDate Text affectedBRITISH STANDARDBS EN ISO 10272-1:2017EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN ISO 10272-1 July 2017 ICS 07.100.30 Super
12、sedes EN ISO 10272-1:2006English Version Microbiology of the food chain - Horizontal method for detection and enumeration of Campylobacter spp. - Part 1: Detection method (ISO 10272-1:2017) Microbiologie de la chane alimentaire - Mthode horizontale pour la recherche et le dnombrement de Campylobacte
13、r spp. - Partie 1 : Mthode de recherche (ISO 10272-1:2017) Mikrobiologie der Lebensmittelkette - Horizontales Verfahren zum Nachweis und zur Zhlung von Campylobacter spp. - Teil 1: Nachweisverfahren (ISO 10272-1:2017) This European Standard was approved by CEN on 1 May 2017. CEN members are bound to
14、 comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CE
15、NELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has t
16、he same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxem
17、bourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-100
18、0 Brussels 2017 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN ISO 10272-1:2017 EBS EN ISO 10272-1:2017EN ISO 10272-1:2017 (E) 2 European foreword This document (EN ISO 10272-1:2017) has been prepared by Technical Committee ISO/TC
19、 34 “Food products” in collaboration with Technical Committee CEN/TC 275 “Food analysis - Horizontal methods” the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest
20、by January 2018 and conflicting national standards shall be withdrawn at the latest by January 2018. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN shall not be held responsible for identifying any or all such patent rights.
21、This document supersedes EN ISO 10272-1:2006. This document has been prepared under a mandate given to CEN by the European Commission and the European Free Trade Association. According to the CEN-CENELEC Internal Regulations, the national standards organizations of the following countries are bound
22、to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal,
23、Romania, Serbia, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. Endorsement notice The text of ISO 10272-1:2017 has been approved by CEN as EN ISO 10272-1:2017 without any modification. ISO 10272-1:2017(E)Foreword vIntroduction vi1 Scope . 12 Normative references 13 T
24、erms and definitions . 14 Principle 24.1 General . 24.2 Enrichment in selective liquid medium . 24.2.1 Detection procedure A 24.2.2 Detection procedure B 24.2.3 Detection procedure C 24.3 Isolation on selective solid medium . 34.3.1 Detection procedure A 34.3.2 Detection procedure B 34.3.3 Detection
25、 procedure C 34.3.4 Detection procedure A, B and C 34.4 Confirmation . 35 Culture media and reagents 36 Equipment and consumables 37 Sampling 48 Preparation of test sample . 49 Procedure. 49.1 General . 49.2 Test portion and initial suspension . 59.2.1 General 59.2.2 Detection procedure A 59.2.3 Det
26、ection procedure B 59.2.4 Detection procedure C 59.3 Enrichment . 69.3.1 Detection procedure A 69.3.2 Detection procedure B 69.4 Isolation 69.4.1 Detection procedure A 69.4.2 Detection procedure B 69.4.3 Detection procedures A, B and C 69.5 Confirmation of Campylobacter 69.5.1 General 69.5.2 Selecti
27、on of colonies for confirmation 79.5.3 Examination of morphology and motility 79.5.4 Study of aerobic growth at 25 C . 79.5.5 Detection of oxidase activity 79.5.6 Interpretation . 79.6 Identification of Campylobacter species (optional) 89.6.1 General 89.6.2 Detection of catalase activity. 89.6.3 Det
28、ection of hippurate hydrolysis . 89.6.4 Detection of indoxyl acetate hydrolysis . 89.6.5 Interpretation . 910 Expression of results 911 Performance characteristics of the method . 9 ISO 2017 All rights reserved iiiContents PageBS EN ISO 10272-1:2017ISO 10272-1:2017(E)Foreword vIntroduction vi1 Scope
29、 . 12 Normative references 13 Terms and definitions . 14 Principle 24.1 General . 24.2 Enrichment in selective liquid medium . 24.2.1 Detection procedure A 24.2.2 Detection procedure B 24.2.3 Detection procedure C 24.3 Isolation on selective solid medium . 34.3.1 Detection procedure A 34.3.2 Detecti
30、on procedure B 34.3.3 Detection procedure C 34.3.4 Detection procedure A, B and C 34.4 Confirmation . 35 Culture media and reagents 36 Equipment and consumables 37 Sampling 48 Preparation of test sample . 49 Procedure. 49.1 General . 49.2 Test portion and initial suspension . 59.2.1 General 59.2.2 D
31、etection procedure A 59.2.3 Detection procedure B 59.2.4 Detection procedure C 59.3 Enrichment . 69.3.1 Detection procedure A 69.3.2 Detection procedure B 69.4 Isolation 69.4.1 Detection procedure A 69.4.2 Detection procedure B 69.4.3 Detection procedures A, B and C 69.5 Confirmation of Campylobacte
32、r 69.5.1 General 69.5.2 Selection of colonies for confirmation 79.5.3 Examination of morphology and motility 79.5.4 Study of aerobic growth at 25 C . 79.5.5 Detection of oxidase activity 79.5.6 Interpretation . 79.6 Identification of Campylobacter species (optional) 89.6.1 General 89.6.2 Detection o
33、f catalase activity. 89.6.3 Detection of hippurate hydrolysis . 89.6.4 Detection of indoxyl acetate hydrolysis . 89.6.5 Interpretation . 910 Expression of results 911 Performance characteristics of the method . 9 ISO 2017 All rights reserved iiiContents PageBS EN ISO 10272-1:2017ISO 10272-1:2017(E)1
34、1.1 Interlaboratory study 911.2 Sensitivity 911.3 Specificity 911.4 LOD50.912 Test report 10Annex A (normative) Diagram of procedures 11Annex B (normative) Culture media and reagents .12Annex C (informative) Method validation studies and performance characteristics .21Bibliography .24iv ISO 2017 All
35、 rights reservedBS EN ISO 10272-1:2017ISO 10272-1:2017(E)11.1 Interlaboratory study 911.2 Sensitivity 911.3 Specificity 911.4 LOD50.912 Test report 10Annex A (normative) Diagram of procedures 11Annex B (normative) Culture media and reagents .12Annex C (informative) Method validation studies and perf
36、ormance characteristics .21Bibliography .24iv ISO 2017 All rights reserved ISO 10272-1:2017(E)ForewordISO (the International Organization for Standardization) is a worldwide federation of national standards bodies (ISO member bodies). The work of preparing International Standards is normally carried
37、 out through ISO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, governmental and non-governmental, in liaison with ISO, also take part in the work. ISO
38、 collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization.The procedures used to develop this document and those intended for its further maintenance are described in the ISO/IEC Directives, Part 1. In particular the different a
39、pproval criteria needed for the different types of ISO documents should be noted. This document was drafted in accordance with the editorial rules of the ISO/IEC Directives, Part 2 (see www .iso .org/ directives).Attention is drawn to the possibility that some of the elements of this document may be
40、 the subject of patent rights. ISO shall not be held responsible for identifying any or all such patent rights. Details of any patent rights identified during the development of the document will be in the Introduction and/or on the ISO list of patent declarations received (see www .iso .org/ patent
41、s).Any trade name used in this document is information given for the convenience of users and does not constitute an endorsement.For an explanation on the meaning of ISO specific terms and expressions related to conformity assessment, as well as information about ISOs adherence to the World Trade Or
42、ganization (WTO) principles in the Technical Barriers to Trade (TBT) see the following URL: www .iso .org/ iso/ foreword .html.This document was prepared by the European Committee for Standardization (CEN), Technical Committee CEN/TC 275, Food Analysis Horizontal methods, in collaboration with ISO T
43、echnical committee ISO/TC 34, Food products, Subcommittee SC 9, Microbiology, in accordance with the agreement on technical cooperation between ISO and CEN (Vienna Agreement).This second edition cancels and replaces the first edition (ISO 10272-1:2006), which has been technically revised with the fo
44、llowing main changes: samples from the primary production stage have been added to the scope; the detection method was extended to include the option of a second enrichment broth (Preston broth), primarily to overcome problems with background flora resistant to third generation -lactams (like cefope
45、razone in Bolton broth); the detection method was extended to include the option of direct plating on mCCDA; the note on the use of closed containers with reduced headspace as an alternative to incubation in a microaerobic atmosphere has been deleted; the confirmation tests on study of microaerobic
46、growth at 25 C and aerobic growth at 41,5 C were replaced by the study of aerobic growth at 25 C; performance testing for the quality assurance of the culture media has been added to Annex B; performance characteristics have been added to Annex C.A list of all parts in the ISO 10272 series can be fo
47、und on the ISO website. ISO 2017 All rights reserved vBS EN ISO 10272-1:2017ISO 10272-1:2017(E)IntroductionThe main changes, listed in the foreword, introduced in this document compared to ISO 10272-1:2006 are considered as minor (see ISO 17468).Because of the large variety of food and feed products
48、, this horizontal method may not be appropriate in every detail for certain products, and for some other products it may be necessary to use different methods. Nevertheless, it is hoped that in all cases every attempt will be made to apply this horizontal method as far as possible and that deviation
49、s from this will only be made if absolutely necessary for technical reasons.When this document is next reviewed, account will be taken of all information then available regarding the extent to which this horizontal method has been followed, and the reasons for deviations from this in the case of particular products. The harmonization of test methods cannot be immediate and, for certain group of products, International Standards and/or national standards may already exist that do not comply with this horizontal method. It is hoped
