1、 , () INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) 32031 2012 Listeria monocytogenes (ISO 11290-1:1996/Amd.1:2004, NEQ) 2014 320312012 II , 1.092 . 1.22009 . -, . , , , 1 - . ( . ) 2 ( 226) 3 , ( 03 2012 . 54-) : - ( 3166) 00497 ( 3166) 00497 KZ KG RU TJ UZ 4 ISO 11290-1
2、1996 Microbiology of food and animal feeding stuffs Horizontal method for the detection and enumeration of Listeria monocytogenes Part 1: Detection method ( . Listeria monocytogenes. 1. ) ISO 11290-1:1996/Amd.1:2004 Microbiology of food and animal feeding stuffs - Horizontal method for the detectio
3、n and enumeration of Listeria monocytogenes - Part 1: Detection method - Amendment 1: Modification of the isolation media and the haemolysis test, and inclusion of precision data ( . - Listeria monocytogenes. 1. -. 1. -, ) (NEQ). 519212002 5 28 2013 . 309- 320312012 1 2014 . 6 - , . () - - . , . , 2
4、014 -, - 320312012 1 Listeria monocytogenes Food products. Methods for detection of Listeria monocytogenes 20140701 1 - Listeria monocytogenes. 2 - : ISO 72182011 . - ISO 11133-12011 . - . 1. ISO 11133-22011 . - . 2. ISO 161402011 . 10444.184 . , , - , 2666885 . 2666985 . 2667091 . 3042597 . - , - 1
5、 , - . (), - () . , , , - , . 320312012 2 3 -: 3.1 Listeria: , - -, , , - . 3.2 Listeria monocytogenes: , - , - . , - . 3.3 Listeria monocytogenes: Listeria monocytogenes . 4 : ALOA Listeria O A; ; ; ; ; ; ; ; TSYEA - ; TSYEB - . 5 L. monocytogenes - (. 5.1, 5.2, 5.3, 5.4 ). 5.1 - ( ) 30 - 24 . List
6、eria -, . Listeria, -. 5.2 , 5.1 ( ) (37 1) 48 . 5.3 , 5.1 5.2, - : ) (): ALOA; ) : , , 5.3 (), O , . ALOA (37 1) (24 3) , (24 3) , L. monocytogenes . 320312012 3 - Listeria - . 5.4 , 5.3, Listeria Listeria monocytogenes (37 1) (24 3) , . 6 , - 6.1 . 6.2 . 6.2.1 .1.1. 6.2.2 UVM .1.2. 6.2.3 ( I) .1.3
7、 6.3 . 6.3.1 .2.1. 6.3.2 (UVM II) .2.2. 6.3.3 ( II) .2.3. 6.4 6.4.1 Listeria O A (LOA) .3.1.1. 6.4.2 Listeria (BRILLIANCE LISTERIA agar) .3.1.2. : 6.4.3 .3.2.1. 6.4.4 .3.2.2. 6.4.5 () .3.2.3. 6.5 - - Listeria 6.5.1 6.5.1.1 - (TSYEA) .4.1.1. 6.5.1.2 - (TSA) .4.1.2. 6.5.1.3 () .4.1.3. 6.5.2 6.5.2.1 -
8、 (TSYEB) .4.2.1. 6.5.2.2 - (TSB) .4.2.2. 6.5.2.3 () .4.2.3. 6.6 Listeria 6.6.1 3 % 10444.1. 6.6.2 10444.1. 6.6.3 . 6.6.4 .8. 6.6.5 .10.1 .10.2. 6.7 Listeria monocytogenes 6.7.1 - 6.7.1.1 .5. 6.7.1.2 .6. 6.7.2 - .11. 6.7.3 - .9. 6.7.4 .7.1 .7.2. 6.7.5 .7.1.2. 6.7.5.1 . 6.7.5.2 . 6.7.5.3 - D- (Methyl
9、D-mannopyranoside). 320312012 4 7 , , , - 10444.1 ISO 7218 . - 0,1 . . -. , (25 1) (50 1) . , : (25 1) , (30 1) , (37 1) . , 44 47 . , - - - 3 . . . . . , . 90 100 . - . -: - S. aureus; - R. qui; - L. innocua; - L. ivanovii; - L. monocytogenes; - E. faecalis; - E. coli. , - , . 8 26668. , - . 9 26670. 10 . 10.1 ( 3) , - 1 : 9. 10.2 (. 10.1) (30 1) (24 3) . 10.3 10.3.1 , 10.2 0,1 3, , - 10 3 (. 6.3). 10.3.2 (. 10.3.1) (48 2) (37 1) . 10.4 10.4.1 (. 10.2) - (. 7.5) (. 6.4.1) , - . 320312012 5 (. 6.4.2 6.4.3, 6.4.4). 10.4.2 , (. 10.3.2), 10.4.1. 10.4.3 (. 10.4.
