GOST 32426-2013 Testing of chemicals of environmental hazard Lemna spp growth inhibition test《环境危险化学品的试验 浮萍属生长抑制 试验》.pdf

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1、 , (OECD, Test 221:2006, IDT) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)324262013, - 1.092 . - 1.22009 . , . , , -, 1 - , , - ( ); 339 , ; 339 , 2 (-)3 , (- 5 2013 . 61-) : ( 3166) 00497 ( 3166) 00497 AZAMBYKZKGMDRUTJTMUZUA - 4 22 2013 . 778- 324262013 - 1 2014 .5 O

2、ECD Test No. 221:2006 Lemna sp.Growth Inhibition ( ). (en). (IDT)6 - , - . () - . , , 2014 -, II 3242620131 12 13 23.1 .23.2 33.3 33.4 34 34.1 .34.2 34.344.4 44.5 .44.6 .54.7 54.8 64.9 64.10 .64.11 74.12 .75.75.1 75.2 85.3 85.4 95.5 - 95.6ECx 95.7 95.8 .10 () Lemna spp. 12 B () .14 C () () 15 19III

3、324262013 : - (Lemna gibba) (Lemna minor). Lemna spp. . Lemna - , , - . , () , . 16, , - . 7. ( ) () . , , , 8.IV 324262013 , Testing of chemicals of environmental hazard. Lemna spp. growth inhibition test 201408011 - Lemna ().2 :2.1 (Biomass): , . , , -, .2.2 (Chlorosis): .2.3 (Clone): , . - .2.4 (

4、Colony): (- -), . .2.5 Cx: , , Lemna x % (, 50 %) - ( ). - EC , ErC EyC .2.6 () (Flow-throwing): , - .2.7 ( ) (Frond): / . . , .2.8 (Gibbosity): , .2.9 (Growth): (, , , ) .2.10 () ( () ) (Growth rate (averagespecific growth rate): .2.11 (Lowest Observed EffectConcentration (LOEC): , (p 0,05) , - . L

5、OEC - , LOEC. , LOEC (, , NOEC).1 324262013 2.12 (Measurement variables): , - . - , .2.13 (Monoculture): .2.14 (Necrosis): ( ).2.15 (No Observed Effect Concentration(NOEC): , LOEC.2.16 (Phenotype): , .2.17 (Response variable): , - , . - , , , , .2.18 (Semi-static (renewal) test): , - .2.19 (Static t

6、est): .2.20 (Test endpoint): , - ( ) . , -, .2.21 () (Test medium): , . - .2.22 (Yield): , - .3 3.1 3.1.1 Lemna - . , - , . -, ( , ), , . , , , , x % - (, 50 %), ECx(, EC50).3.1.2 - ( ) -. , (, 50 %), ErCx(,ErC50). , , x % (, 50 %), ErCx(, ErC50).3.1.3 , . - . , (, 50 %), - ErCx(, EC50). , , , x % (, 50 %), ErCx(, ErC50).3.1.4 (LOEC) , (NOEC).2 3242620133.2 3.2.1 .3.2.2 , , - , , pKa,Kow, . , - . - , . , - , , , , .3.2.3 pH , , . (. 4.4). - , , 8.3.3 , 2,5 (60 ), - 0,275 1. , , 5. . 5.1.3.4 , , 3,5-, 7. , - , .4 4.1 4.1.1 , , . , - , , . ,

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