GOST 32473-2013 Testing of chemicals of environmental hazard Fish acute toxicity test《环境危险化学品测试 鱼类急性毒性试验》.pdf

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1、 , () INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) 32473 2013 , (OECD, Test No 203:1992, IDT) 2014 324732013 II , - 1.0-92 . 1.2-2009 . , . , , , 1 , , - ( ) 2 3 , 61 5 2013 .) : ( 3166) 004-97 ( 3166) 004-97 AM BY KG MD RU TJ 4 OECD Test 203:1992 Fish, Acute Toxicity Te

2、st ( ). (en). (IDT). 5 22 - 2013 . 783- 324732013 - 1 2014 . 6 - , . ( ) - - . , , 2014 - , - 324732013 1 , Testing of chemicals of environmental hazard Fish, Acute Toxicity Test 20140801 1 . 2 : 2.1 (Static test): , . 2.2 (Semi-static test): , - ( , 24 ). 2.3 (Flow-troughn test): , . 2.4 LC 50 : ,

3、 - , 50% , . 3 96 . - 24-, 48- , 72- 96- , , - 50% , (LC 50 ). 4 4.1 - . - . 4.2 : - ; - ; - ; - pK a ; - / K ow ; - ; - . - , , . 5 : - 10% ( . - 10 ) ; 324732013 2 - - ; - 60% - ; - , , , 80% - . 20%, . 6 6.1 , - : - ; - ; - ; - , , - . 6.2 6.2.1 - . , , , , - , , . , . 6.2.2 , , 1. - , - , , . -

4、 , , - , . 1 ( 0 ) , * Brachydanio rerio (Teleostei, Cyprinidae) (Hamilton-Buchanan) 21 25 2.0 1.0 Pimephales promelas (Teleostei, Cyprinidae) (Rafinesque) 21 25 2.0 1.0 Cyprinus carpio (Teleostei, Cyprinidac) (Linnaeus) 20 24 3.0 1.0 Oryzias latipes (Teleostei, Cyprinodontidae) (Temminck and Schlegel) 21 25 2.0 1.0 Lepomis ma rochirus (Teleostei, Centrarchidae) (Rafinesque) 21 25 2.0 1.0 Poecilia reticulata (Teleostei, Pocciliidae) (Peters) 21 25 2.0 1.0 Oncorhynchus mykiss (Teleostei, Salmonidae) (Walbaum) 13 17 5.0 1.0 * , , .6.3 6.3.1 12 . - 324732013 3 , ,

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