1、 , () INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) 32538 2013 , - (OECD Test No 305:1996, IDT) 2014 32538-2013 II , - 1.092 . - 1.22009 . -, . , , . 1 - - , , - ( ); 339 - , 2 3 , ( 14 2013 . 44-2013) : ( 3166) 004-97 ( 3166) 004-97 AM BY KG MD - RU TJ 4 OECD Test No 305
2、 Bioconcentration: Flow-through Fish Test (: ). (en). (IDT). 5 22 2013 . 771- 325382013 - 1 2014 . 6 32538-2013 III , - . - () -. , - - , 2014 -, - 32538-2013 IV , ( 1990), , (305 E) . 305 E , - 1. , 305 E . 305 (305 E) . . - , , (. 5.2) . 32538-2013 1 , - Testing of chemicals of environmental hazar
3、d Determination of bioconcentration in flow-through fish test 2014 - 08 - 01 1 . , , - . - , log Pow 1,5 6,0 (4), (log Pow 6,0). , , , (BCFss) - , . - log Pow 9,0 . , : - (k1), (k2) ( (BCFss). - , - . - (, ), - , . , -, , . , , - , , , . - , . , , - . - . 2 , : 2.1 / (bioconcentration/ bioaccumulati
4、on): - / ( ) . 2.2 () k2(depuration (loss) rate constant): , ( - ) , - , (k2 -1) 32538-2013 2 2.3 k1(uptake rate constant): , - / ( ) , - (k1 -1). 2.4 / ow(octanol-water partition coefficient): - - , -, ow. ow - . 2.5 / (plateau/steady-state): , , , (f), - , f, - , , , , , 20 %, - . , . - . 2.6 (exp
5、osure/uptake phase): , . 2.7 () (post-exposure or depuration (loss) phase): , , - , , ( ) ( ). 2.8 (BCF ) (bioconcentration factor): - / (f / ( ) (w / ( ) . 2.9 (ss ) (steady state bioconcentration factor): , , . 2.10 (bioconcentration factor): - (BCFK), (k1/k2 . ). 3 3.1 : () (-). - . - , , . , , -
6、 (, BCF 10). - / ( ) . - , , - . 3.2 28 , . , 20 %. - , . - . (BCFSS) (Cf), (Cw) - , BCFK, 32538-2013 3 (k1) (k2) , - 1. 3.3 28 , 60 , , ; - . 3.4 , () ( , ), , , , - , . 3.5 -. , (-, , ), , . - , -. , , (. log Pow 3) - . , , , . 4 4.1 4.1.1 - : - 3; - /, Pow, 4, 5, 9; -, 6; - , , 10; - ( , log Pow) 7; - 8; - ( ), 11. 4.1.2 , , LC50. 4.1.3 - ,
