GOST 32648-2014 OECD guidelines for the testing of chemicals Genetic Toxicology Sex-linked Recessive Lethal Test in Drosophila melanogaster《化学品试验OECD指南 遗传毒理学 黑腹果蝇伴性隐性致死试验》.pdf

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1、 , () INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION (ISC) 32648 2014 : Drosophila melanogaster (OECD, Test 477: 1984, IDT) 326482014 II , 1.092 . 1.22009 . , , . , , 1 ( ); - , , ( ); 339 , 2 3 , ( 28 2014 . 65) : ( 3166) 004 97 ( 3166) 004 97 AM BY MD RU TJ UZ - 4 29 2014 . 12

2、35- 32648-2014 01 2015 . 5 OECD Test 477:1984 Genetic Toxicology: Sex-linked Recessive Lethal Test in Drosophila melanogaster (, 477:1984 : Drosophila melanogaster). (en). (IDT) 6 , . () . , , 2015 , 326482014 III : -, , ; - ; - () ; -; - / ; - ( ); - ( ). 326482014 1 : Drosophila melanogaster OECD

3、guidelines for the testing of chemicals Genetic Toxicology: Sex-linked Recessive Lethal Test in Drosophila melanogaster 2015 06 01 1 Drosophila melanogaster () , . 800 -, 80% -. - 1/5 2. 2 : 2.1 (Lethal mutation): , . 2.2 (Recessive mutation): , . 2.3 (Reference substances): , : - ; - N-. 2.4 (Sex-l

4、inked): (X Y) . , -. 3 - Drosophila melanogaster , . , , . , . 4 Drosophila melanogaster 2 3. . . . , 1 . 4.1 4.1.1 Muller-5. -. 326482014 2 4.1.2 . (, Tween-60 Tween-80), . . 4.2 4.2.1 , . . 0,7 %- NaCl . 4.2.2 , . , , , . 4.2.3 . , , . 4.3 ( 35 ) Muller-5 , ( -) . 23 , . , , , . (F1) ( ) . F2 . F1

5、 - (. ), , . . . , , , , , , . . 5 5.1 , , , , . . . . 5.2 , . , . , , 326482014 3 , -. , . 5.3 : - : ; ; ; ; F2; F2 ; ; , ; - : ; , , () ; - ; - - (, ); - . 5.4 () , . , . 326482014 1 OECD Test 477 Genetic Toxicology: Sex-linked Recessive Lethal Test in Drosophila melanogaster 2 F.H. Sobels and E.

6、Vogel, Mutation Res. 41, 95-106 (1976) 3 F.E. Wrgler, H. Sobels and E. Vogel, in Handbook of Mutagenicity Test Procedures (edited by B.J. Kilbey, et al.), pp. 335-373, Elsevier, Amsterdam (1977) 661:615.099 13.020.01 : , , , , , Drosophila melanogaster 12.01.2015. 60x841/8. . . 0,93. 33 . . 199. , 123995 , ., 4. www.gostinfo.ru infogostinfo.ru

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