GOST 32713-2014 Alcoholic beverages and raw materials for its production Identification Enzymatic method for determination of D-malic acid mass concentration《生产用酒精类饮品和原材料 识别 测定D-羟基.pdf

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2、 .26 , , , .37 .38 .39 .510 511 , . 612 713 , 7III 327132014 D- Alcoholic beverages and raw materials for its production. Identification.Enzymatic method for determination of D-malic acid mass concentration 201601011 , , , -, () , () , (-), , ( ) D- - , /3. 0,05 10,00 /3.2 : 12.0.00490 . - . 12.1.00

3、491 . . 12.1.00588 . - 12.1.00776 . . - 12.1.01979* . . - 12.4.00983 . -. . OIML R 76-12011 . - . 1. . 177074 ( 104283, 478880) . -, , , . 451787 . , - 59622013 . 670972 . 1202676 . * 12.1.0192009.1 327132014 2533682 . , 2631384 . 2916991 ( 64877) . 2922791 ( 835-181) . . I. 317302012 . 320952013 .

4、- - , 1 , . (), - () . - , , , , .3 , , 3.1 :3.1.1 D- D (+)- : . , , , : L ()- .3.2 : c98-; c98-, ;D- D-;U , ; ;HEPES- 4-(2-)1- - 0,03 /3( = 9,0).4 D- - D- (1) 334 , 340 , 365 , D- .D-D- + c171 + CO2+H+H+ . 5 /3 D- .5 D- :- , (20 c177 5);- , % 3080;- , 220c45c433322;- , (50 c177 1).2 3271320146 , ,

5、, 10 334 , 340 , 365 , - c1771%. 1 . OIML R 76-1, 210 , 0,0001 , c1770,001 . 1-2-1-0,5, 1-2-1-1, 1-2-1-2, 1-2-1-5 1-2-1-10 3 29227. 2-2-20 29169. c1771 %, 0,05 3, 0,1 3,13 -53. 2-100-2, 2-250-2, 2-500-2, 2-1000-2 1770. 50 3 25336. 1-50-2, 1-100-2 1770. , , . 25336. 25 100 3 25336. . 8 10 23 . 90 %.D

6、- , 13 U/3.4-(2-)1- , , - 98 %.D (+)- , , 98 %. . 6709. , 4517. 5962. 12026. , , , - .7 7.1 , , , , (-) , () , (), , -, 31730.7.2 , - , 26313.8 8.1 -.8.2 8.2.1 , , - , -. .3 327132014 , - D- .8.2.2 , , 10 %, - . , - ! -. .8.3 8.3.1 HEPES- ( 1)0,170 4-(2-)1- 20 3- , . . 1 4 C 20 20 C 60 . 1 1 .8.3.2 ( 2)0,21 4 3 , 53. . 2 4 C 20 20 C 60 . 2 1 .8.3.3 D- ( 3)3 D- 0,6 3 , 1 3. - D-. 3 2 C8 C 5 . 3 1 .8.3.4 10 % 10 % 4517. .8.4 8.4.1 ( ) , , . - - 32095.8.4.2 . 25 3 0,2 , 1015 3 . 15 . , , 50 3, 10 3, - , . - .8.5 - , 10 . - D- .4 3271320149 . .

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