GOST 32916-2014 Milk and milk products Determination of vitamin D mass fraction by high performance liquid chromatography method《奶和奶制品 采用高效液相色谱法对维生素D质量分数的测定方法》.pdf

1、 D , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)329162014, - 1.092 . 1.22009 . -, . , , 1 - ( )2 (-)3 , ( 5 2014 . 46) : ( 3166) 00497 ( 3166) 00497 KGRUUZ 4 12 2014 . 1976- 329162014 - 1 2016 .5 -, - . () . , - - , 2015 -, II 3291620141 .12 13 24 .25 , , .26 .37 38 49

2、 .510 .511 512 .613 , .6 () D 7III 329162014 D ilk and milk products. Determination of vitamin D mass fraction by high performance liquid chromatography method1 - D ( ). 0,01 1,0 1.2 : 12.1.00491 . . 12.1.00588 . - 12.1.00776 . . 12.1.01979* . . - 12.4.00983 . . . 12.4.02175 . . IML R 76-12011 . . 1

3、. . 177074 ( 104283, O 478880) ., , , . 416676 . . O 5725-62003* ( ) . 6. 670972 . 980584 . 1830087 . 2436380 . . * 12.1.0192009.* 5725-62002.1 329162014 20160101 2533682 . , 2680986 . , 2775288 - , -. 2849890 . . 2922791 ( 835-181) . . 1. - , 1 , - . - (), () . , , , , .3 :3.1 D: ( D3), (1).4 - - ,

4、 D . - ( ) . D , . .5 , , , :- 0,400,46 , 2530 , , 5 ;- - 265 280 c1771;- ;- ;- ( ), ( ) c1771%;- . . 18 500 /6 ; 190 1100 , - 1 %. OIML R 76-1 , , , c1770,001 . - 27752. 0 100 , 1 28498. .2 329162014 10 . 0,5 . 0,100 3. 2-100-2 1770. 1(2,3)-1(2)-1-1(5,10,25) 29227. -2-5-0,1; -2-10-0,2; -2-20-0,2 17

5、70. 1-25-2 1770. -1-100-29/32, -1-250-29/32, -1-500-29/32 25336. -1-100, -1-250 25336. -56-80 25336. -1-300-29/32 25336. , . ., 99,98 %. 24363, . ., 5 /3. , . 99,5 % 9805. 99,9 % - , , . 96 % 18300. ( D3) 98 %. 6709. , , - - , .6 6.1 26809.6.2 , , - (4 c177 2) .7 7.1 D3 100 3 (100 c177 1) D3, - . -

6、. 1 (4 c177 2) c176 .7.2 D37.2.1 1 3 D3, 7.1, 100 3, . . . 10 /3. D3 - 10265, . D3(CD3,/3) , CD3= 265c215 106/100 c215 485, (1) 265 D3 265 ;106 ;100 1 3;485 D3 1 100 3(10 /) 265 10 .3 3291620147.2.2 D3 0,110,0 /3. 1,0 10,0 3 D3(7.2.1) - 100 3 . - D3 0,1 1,0 /3. 0,5 1,0 3 D3(7.1) 100 3 . D3 5,0 10,0 /3.7.3 7.3.1 , 30 % , 20 -, 0,001 , 100250 3, 25 3 5 /3,1 ( ) 25 3 . 70 80 30 . -. (V), 10 3(V2) 1:1 (V/V). D, () . - 50 %. . D - 2 3. (V1) - .7.3.2 , 30 % , 1015 , 0,001 , 250500 3 , 7.3.1.7.4 7.4.1 , - . . D . D3 1 /3(7.2.2) - D3.7.4.2 :- (23 c177 2) ;- 0,71,0 3/;- 20 ;- .7.5 . D3,-