1、 , : (OECD, Test No 214:1998, IDT) , ()INTERSTATE COUNCIL FOR STANDARDIZATION, METROLOGY AND CERTIFICATION(ISC)330392014, 1.092 . 1.22009 . , - . , , -, 1 - , , ( ) - , 52 ()3 , (- 5 2014 . 46) : ( 3166) 00497 ( 3166) 00497 AMKGMDRUUA - 4 12 2014 . 1988- 330392014 1 2015 .5 OECD, Test No 214:1998Hon
2、eybees, Acute Contact Toxicity Test (, 214:1998 : - ) (en). (IDT)6 -, - . () . , - - , 2015 -, II 3303920141 12 13 14 .15 26 .27 38 4 .5III 330392014 , . - (EPPO) (Apis mellifera) 1. - , (ICPBR) 5- , () 1993 . 2. 3, 4, 5.IV 330392014 , : Testing of chemicals of environmental hazard. Honeybees, acute
3、 contact toxicity test 201508011 - .2 :2.1 (acute contact toxicity): , - - 96 .2.2 (dose): . - () (/).2.3 LD50 ( ) (median lethal dose) contact): - , 50 % .2.4 (mortality): , .3 3.1 ,- . - . . , - , - , 6. (. .) .3.2 ( ) .3.3 2.4 , , ( ) . 48 . -1 330392014 2448, , 10 %, 96 (). . LD50 24 48 , , 72 9
4、6 .5 , :- 10 % ;- LD50.6 6.1 , . . -, . -, , , . . . ,. , - () 1 . , , , . ., 4 .6.2 , , , , , . . - : 10 (). , . . .6.3 , , - . 500 / (50 % /), (ad libitum). (50 , 10 , 2 ).6.4 6.4.1 . - . .6.4.2 ( ). , (, -). , , - , , , , (Agral, Citowett,Lubrol, Triton, Tween).6.4.3 - . , - , 2 : , , , /.6.4.4 ,
5、 - . 2 3303920142 . , , . .7 7.1 7.1.1 LD50 95 %- . 5 -, , 2,2 - LD50. ( ) , . - .7.1.2 3 10.7.1.3 , , , , 10 . - .7.2 . - LD50 3 , , , 3 () 10 . , :LD50(24) = 0,100,30/7., (, ).7.3 . . 1 , , . - , . .7.4 25 c177 2C. 5070 % .7.5 48 . 10 % 24 48 , 96 , -10%.7.6 4 24 48 . - , 24- 96 , , 10 %. - .7.7 (, ) - 100 ./, , LD50 -. 3 330392014 , , - . . (. 7.6) .8 8.1 , . , , . (, -, , - ) 8, 9. (, 24 48 , - 72 96 ) , (LD50) 95 %- . 9, 10. LD50 .8.2 :
