1、 549492012( 6867:2000) ISO 6867:2000Animal feeding stuffs Determination of vitamin content Method using high-performance liquid chromatography(MOD) 1 -(),42 004 , -,3 -312012.211-4 6867:2000 . . (ISO6867:2000Animalfeedingstuffs Determinationofvitamincontent Methodusinghigh-performanceliquidchromatog
2、raphy),. 5.85.19, ,. , 5.48.3, , 2 -,- 1.5 ( 4.2 4.3), ,. -,1.5(-3.5),1.5(3.6). 5 1.02012 ( 8). ( 1 ) , -.()- .,- (gost.ru),2014 , -II 5494920121 .12 13 24 25 , .26 .47 .48 .49 .610 .611 7() 8() C911III 549492012 Animal feeding stuffs. Determination of vitamin content by high-performance liquid chro
3、matography method 201307011 - (DL-c97-) - ().2 : 5141999 ( 649898) , , . ( 6498:1998, MOD) 5156899 ( 3310-190) . 516522000 . 532282008 . 1. - . 177074 ( 104283, 478880) ., , , . 416676 . . . 670972 . 929374 ( 243573) . 1202676 . 13496.080 , . 2436380 . . 2533682 . , - 2922791 ( 835-181) . -. 1. - ,
4、1 , - . - , , . , -1 549492012( 6867:2000) , (). , , , , , . , -, , , .3 - , , . - , DL-c97- , (/).4 4.1 6709.4.2 ()24363,50%500 1000 3(. 5.17), (. 4.1) .4.3 (25) 51652 95 % .4.4 .4.5 , 40 C 60 C, - 20 /3.4.6 : DL-c97-, 96,0 %. - (. 8.5.2).4.7 1,4- .4.8 (N2SO4) 4166,.4.9 , = 100 /3.4.10 , , 9293.4.1
5、1 1,4- (.4.7)(. 4.4) 30:970 . (.5.5).4.12 (25) 51652 96 %.4.13 (3) . . . . . .5 , , , :5.1 , :5.1.1 , 1,5 3/.5.1.2 .5.1.3 250 4,6 , , - . , 5000, - kc162 0,8 DL-c97-. 510. , .5.1.4 , , 326 , () 293 , ( ).5.2 .5.3 40 C.2 5494920125.4 (. 1), :5.4.1 1000 3 1770 .5.4.2 , , , (. 5.4.3), .5.4.3 U- 1000 3.
6、1 1000 3; 2 ; 3 ;4 , ; 5 ; 6 1 , 1, , .5.5 0,45 (. 4.11) .5.6 , 1,0 .5.7 - ( - ), , 8.5.2, 10 .5.8 1,0 51568.5.9 4,0 .5.10 53228 500 - c177 0,001 .5.11 -2-1000-42 25336.5.12 25336.5.13 -1(2)-1000 25336.5.14 -1(3)-100 25336.5.15 12026 .5.16 -2-250-34 25336.5.17 1(2)-25(100, 1000)-2 1770.5.18 1(2, 3,
7、5)-1(1, 2, 2)-1-2 29227.5.19 1(2)-25(50, 100, 250, 1000) 1770. 5.85.19 -, .3 5494920126 13496.0. , . , .7 51419. (. 5.6) 1,0 (. 5.8). . , 4,0 (. 5.9) .8 8.1 - , ,. . .8.2 (. 5.10) (50 c177 0,1) , 7, 1000 3(. 5.11). (. 5.19) 200 3(.4.3),. (. 5.18) 2 3 (. 4.9), - , 50 3 (. 4.2) . (. 5.12) (.5.2). 30 , . - . . (. 5.4.1).8.3 8.3.1 25 3 (.4.3) (. 5.4.1). 125 3 (. 4.5) 250 3 (. 4.1), . 1 , - . . , -