1、 14502-12010 ISO 14502-1:2005Determination of substances characteristic of green and black tea Part 1: Content of total polyphenols in tea Colorimetric method using Folin-Ciocalteu reagent(IDT) 27 2002 . 184- , 1.02004 . 1 ( ) 4,2 451,3 -302010.676-4 14502-1:2005 . 1. .Folin-Ciocalteu(ISO14502-1:200
2、5Determinationofsubstances characteristic of green and black tea Part : Content of total polyphenols in tea ColorimetricmethodusingFolin-Ciocalteureagent).,-,-. -1.5(3.5).- -,5 6 . , -.().-, ,2012 , -II 14502-120101 .12 13 .14 15 .26 .37 38 38.1 38.2 38.3 .38.4 .38.5 .48.6 49 .410 .510.1 510.2 510.3
3、 .511 5 () 6 B () 7 () ( ) .8 9III 14502-12010O 14502-1 ISO/TC 34, , - SC 8, .IV 14502-12010 Tea. Method for determination of total polyphenols content 201201011 Folin-Ciocalteu.2 . , . ( ). 1572:1980 . (ISO 1572:1980, Tea Preparation of ground sample of known dry matter content) 3696:1987 . (ISO 36
4、96:1987, Water for analytical laboratory use Specification and test methods) 7513:1990.(- 103 ) (ISO 7513:1990, Instant tea in solid form Determination of moisture content (loss inmass at 103 C)3 70 %- 70 . 10 %- . - Folin-Ciocalteu. Folin-Ciocalteu - - , OH- . , - 765 , . - Folin-Ciocalteu - , - .4
5、 .4.1 , 1- 3696.4.2 .1 14502-12010 4.3 .4.4 , 70 %- (.) 700 3 4.3 1000 3, 4.1 .4.5 Folin-Ciocalteu.4.6 Folin-Ciocalteu , 10 %- (.) 20 3 Folin-Ciocalteu 200 3, 4.1 . .4.7 , 7,5 %- ( ) (37,50c1770,01) (Na2CO3) 500 3.- 4.1, - , , 4.1 . 1 .4.8 , , 1/3 (0,110 c1770,001) (M=188,14) -1003,4.1,. . - . , 103
6、 C, - .4.9 , A E 4.8 , 1, 100 3, 4.1 - . . 1 , , 3 , /3A 1,0 10B 2,0 20C 3,0 30D 4,0 40E 5,0 505 .5.1 c177 0,001 .5.2 , (70 c177 1) .5.3 - 5 3 .5.4 3500 /.5.5 , 765 10 , .5.6 .5.7 50, 100, 200, 500 1000 3.5.8 .5.9 10 3 .2 14502-120105.10 10 3 0,1 3. 5.9 5.10 15 %- (.) , 100 . , . .6 , - . 1839, 7516
7、.7 1572 , . , .8 8.1 (. 10.2), 8.28.6.8.2 ( 103 ), 1572 7513 .8.3 8.3.1 (0,500 c177 0,001) 7 - 5 3.8.3.2 (0,200c1770,001) 7 - 5.9.8.4 8.4.1 8.4.1.1 (8.3.1) 25 3 (- 60 ). .8.4.1.2 5,0 3. 4.1 .8.4.2 8.4.2.1 - 5.3 4.4 - 5.2 70 30 .8.4.2.2 8.3.2 70 . 5,0 3 8.4.2.1 , 5.8.8.4.2.3 10 , 5 10 . .3 14502-1201
8、08.4.2.4 . , 10 3500 /.8.4.2.5 .8.4.2.6 8.4.2.28.4.2.5. , 10,03 .8.4.2.7 8.4.2.6 24 4 . . .8.5 1,0 3 8.4.1.2 8.4.2.6 100 3. 4.1 .8.6 8.6.1 1,0 3 A, B, C, D E 4.9 5.10. 10, 20, 30, 40 50 .8.6.2 1,0 3 - 5.10. .8.6.3 1,0 3 8.5 .8.6.4 5,0 3 Folin-Ciocalteu 4.6 .8.6.5 38 Folin-Ciocalteu 4,0 3 4.7 . .8.6.6 60 , - 5.5 4.1 - 10 765 .8.6.7 0,01. , , . , , . - ,- 8.5.9 9.1 0,1 m,13- A, , , D E 4.9, 8.6.1, m =mVwDM std010000100 100c215c215 c215c215, m0 , - 4.8, ;V , - A, , , D E 4.9, 3;wDM,std , %.9.2 -A,D E(4.9),9.1,
