GOST R ISO 16256-2015 Clinical laboratory testing and in vitro diagnostic test systems Reference method for testing the in vitro activity of antimicrobial agents against yeast fung.pdf

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1、 162562015 - IN VITRO in vitro , ISO 16256:2012Clinical laboratory testing and in vitrodiagnostic test systems Reference method for testing the in vitro activityof antimicrobial agents against yeast fungi involved in infectious diseases(IDT) 1 ( -) -, 42 380- - in vitro3 - 27 2015 . 300-c4 16256:201

2、2 - in vitro. - in vitro , (ISO 16256:2012 Clinical laboratory testing and in vitro diagnostic testsystems Reference method for testing the in vitro activity of antimicrobial agents against yeast fungi involvedin infectious diseases)5 1.02012 ( 8). ( 1 ) , -.() - . , - (www.gost.ru) , 2015 , - - II

3、1625620151 .12 23 43.1 43.2 .43.3 43.4 .73.5 73.6 .83.7 83.8 .83.9 94 9() RPMI-1640.12() 0,5() .14() .1415III 162562015 in vitro , - , , - . , - . - () - . :- ;- ;- , - ;- , , , . ( ) ( ), - . ( /), - in vitro , . - . - , ., , - , (. . c177 1 ). , , - (, ), . , -. - . , , Clinical and Laboratory Sta

4、ndards Institute ( ) (CLSI) 1 European Committee on Antimicrobial Susceptibility Testing (- ) (EUCAST) 2. , , 2 / 3. - . , - , , , , , (CLSI ) (EUCAST ). .IV 162562015 -IN VITRO in vitro , Clinicallaboratorytestingandin vitrodiagnostictestsystems.Referencemethodfortestingthein vitroactivityofantimic

5、robialagentsagainstyeastfungiinvolvedininfectiousdiseases 20160601! - , . - , . , - - .1 - , , Candida spp.Cryptococcus neoformans. , , - , B. dermatitidis / H. capsulatum, capsulatum.,()- , . - CLSI M38 EUCAST E.DEF9.14,5,6,7,8., . (CLSI ) 1; (EUCAST ) 2. , . - : () (susceptible, S), , ()(susceptib

6、le dose-dependent, S-DD), () (intermediate, I), () (non-susceptible, NS), () (resistant, R). , . ; -,CLSIEUCAST, , 2,9. .1 162562015 2 :2.1 (antifungal agent): , - , , . , -.2.2 (antifungal agents-properties)2.2.1 (potency): , - . (/), - x () , , .2.2.2 (concentration): .1 /.2 / = /, /.2.3 (stock so

7、lution): , -.2.4 ; (minimum inhibitory concentration,MIC): , in vitro . /.2.5 (breakpoint,BP): , : ()(susceptible, S), , () (susceptible dose-dependent, S-DD), - () (intermediate, I), () (nonsusceptible, NS) - () (resistant, R). - , (, CLSI and EUCAST) 1, 2, 9.2.5.1 (visual reading pathway)2.5.1.1 ;

8、 (susceptible, S): , in vitro - , .1 - -.2 (, - , ).2.5.1.2 , ; (susceptibledose-dependent, S-DD): , in vitro , -invivo,.1 , - -.2 , , , - , -, .3 (, - , ).2 1625620152.5.1.3 ; (intermediate,I): , . , , , , -, ; , - .2.5.1.4 ; (nonsusceptible,NS): , - , -, , , ( ). , - .2.5.1.5 ; (resistant, R): , in vitro ,.1

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