1、 205412011 - ISO 20541:2008Milk and milk products Determination of nitrate content Method by enzymaticreduction and molecular-absorption spectrometry after Griess reaction(IDT) 27 2002 . 184- , 1.02004 . 1 - ( ) , - 42 335 - 3 12 2011 . 775-4 20541:2008 - . . - - (ISO 20541:2008 Milk andmilk product
2、s Determination of nitrate content Method by enzymatic reduction and molecular-absorptionspectrometry after Griess reaction). - - , 5 , - . () . - , , 2013 , - - II 2054120111 .12 13 14 .25 26 .37 .48 .48.1 , 48.2 48.3.48.4 .59 .59.1 .59.2 59.3 .69.4 .69.5 710 710.1 ( ) .710.2 / .810.3 .811 .811.1 .
3、811.2 .811.3 912 9 () .10 () ( ) .1314III 205412011 . - Milk and milk products.Determination of nitrate content.Method by enzymatic reduction and molecular-absorption spectrometry after Griess reaction 201301011 - -. , , , , , , , , . 0,2 /3. , .2 : 565 . , , - . (ISO 565 Test sieves Metal wirecloth
4、, perforated metal plate and electroformed sheet Nominal sizes of openings) 648 . (ISO 648 Laboratoryglassware Single-volume pipettes) 835 . (ISO 835Laboratory glassware Graduated pipettes) 1042 . (ISO 1042 Laboratoryglassware One-mark volumetric flasks) 3696 . (ISO 3696 Water for analytical laborat
5、ory use Specification and test methods)3 .3.1 (nitrite content): , , .3.2 (nitrate content): , , . ( NO3c45) .1 205412011 4 .- , (. 1 2). - . N-(1-)- - ( ), ( ), 540 ( Hg 546 ). -, . -.1 9.2.1 9.2.2.2 , , .3 , .5 , , 3 3696, , . .5.1 , c (NaOH) = 1 /3.5.2 , c (NaCl) = 0,9 /100 3.5.3 ,c11420(HCl) = 1
6、,19 /3.5.4 ,c (HCl) = 2 /3 160 3 (5.3) 700 3 - 1000 3 (6.4) . . .5.5 5.5.1 I: (II) , c (K4Fe(CN)6 c215 3H2O) = 150 /3. 15,0 (II) 100 3 (6.4). .5.5.2 II: , c (ZnSO4c215 7H2O) = 300 /3. 30,0 100 3 (6.4). - .5.6 5.6.1 (NaNO2)(75,0 c177 0,1) ( 102 C 2 ) 100 3 . , . 500 . , , 0,05 5,0 /3. .5.6.2 (KNO3) (
7、81,5 c177 0,1) ( 102 C 2 ) 100 3 . , . 500 . , , 0,05 5,0 /3. 4 C .2 2054120115.7 , pH = 7,5 (57,6 c177 0,1) (K2HPO4c215 3H2O) 100 3 . , . (17,0 c177 0,1) (KH2PO4) 50 3 . , . pH (6.18) pH pH 7,5 . 4 C .5.8 NADPH/FAD (5,6 c177 0,1) 3- (), (c98-NADPH-Na4, 98 %), (80,0 c177 0,1) -, (FAD-Na2, 88 %) 25 3
8、 . (5.7). - (5.7) . NADPH/FAD .5.9 (NR) 65 (NR) Aspergillus niger (EC 1.6.6.2, , 0,4 /) 10 3. 5 3 . 4 C .5.10 5.10.1 I: (NH2C6H4SO2NH2) 400 50 3 (6.4). , . . (5.4) . . 4 C I .5.10.2 II: N-(1-) (C10H7NHCH2CH2NH2c215 2HCl) 50 N-(1-) 50 3 (6.4). . 50 3 . . 4 C II .5.11 . - ( , 5.8).6 . , , :6.1 , 0,1 .
9、6.2 .6.3 100, 500 1000 3 .6.4 25, 50, 100 1000 3 1042, A.6.5 1, 2, 5 10 3 648, A, 835. .6.6 , .6.7 20 50 3.6.8 20 50 3.6.9 , (6.10) (6.21) 3000g.6.10 15 .6.11 0,45 .3 2054120116.12 .6.13 540 546 .6.14 ( ), 1 .6.15 , . .6.16 , 200 , - 500 565.6.17 .6.18 pH, pH- / -, 20 C.6.19 , (70,0 c177 0,5) C.6.20 .6.21 , MWCO 5000 D, 4 3, - .7 , . - 707.,
