1、 KSKSKSKS KSKSKSK KSKSKS KSKSK KSKS KSK KS 2006 11 20 http:/www.kats.go.krKS H ISO 118161 1： KS H ISO 11816 1： 2006 (2011 ) H ISO 11816 1： 2006 : ( ) ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ： 2001 12 31 ： 2006 11 20 ： 2011 12 29 2011-0652 ： ： ( 02-509-7273) (http:/www.kats.go.kr). 10 5 , . ICS 67
2、.100.10 KS 1： H ISO 11816 1： 2006(2011 ) Milk and milk products Determination of alkaline phosphatase activity Part 1： Fluorimetric method for milk and milk-based drinks 1997 1 ISO 11816 1 Milk and milk products Determination of alkaline phosphatase activity using a fluorimetric method Part 1： Milk
3、and milk-based drinks ISO/TC 34 (SC 5), (IDF) 2006 (ISO 11816 1/IDF 155 1) . KS H ISO 11816 1/IDF 155 1 KS H ISO 11816 1： 2001 . ISO 11816/IDF 155 . 1： 2： A . 1. (whole milk), (semi-skimmed milk), (skimmed milk) (flavoured milk) (ALP EC 3.1.3.1) . , . 2 000 mU/L . 2. . 2.1 (ALP) (mU/L) . 2.2 1 1 mol
4、 3. . 2-2-benzothiazolyl-6-hydroxybenzothiazole . (ALP) 3 38 . H ISO 11816 1： 2006 2 . 3 , 1 38 1 1 ( , 2 ) . 4. , . 4.1 (fluorophos) (1) Fluorophos 144 mg . 2-2-benzothiazolyl-6-hydroxybenzothiazole (Fluorophos) . Fluorophos 28 2 . (1) 4.14.5 5.15.4 (5.3.3 ) Massachusetts 02062, Norwood, 2 Technolo
5、gy Way Advanced Instrument Fluorophos Test System . Fluorophos Test System . . , . ISO IDF . 4.2 240 mL pH 10.0 (DEA； diethanolamine) 2.4 mol/L . 28 2 . 4.3 (working) Fluorophos (4.1) (4.2) . (4.2) (240 mL) Fluorophos (4.1) (144 mg) 3 . . 30 . 8.4.1.1 . 28 60 , 38 8 . 1 200 (8.4.1.1.5 ). (240 mL) 11
6、5 . 4.4 DEA (4.2) (Fluoroyellow； FY) 2-(2-benzothiazolyl)- 6-hydroxybenzothiazole. 28 18 . 4.4.1 A Fluoroyellow0 mol/L 4.4.2 B Fluoroyellow17.2410 3 mol/L 4.4.3 C Fluoroyellow34.4810 3 mol/L 4.5 Fluoroyellow34.4810 3 mol/L 5. . 5.1 (1) (381) (cuvette) , 440 nm H ISO 11816 1： 2006 3 (excitation) , 44
7、0 nm 520560 nm (emission) Fluorophos (1). . 5.2 12 mm, 75 mm . 5.3 5.3.1 (fixed-volume dispenser) 2.0 mL . 5.3.2 0.075 mL . 5.3.3 2 mL . 5.4 (381) . 5.5 (2) (2) (Parafilm) . , ISO IDF . 5.6 (vortex) 5.7 (631) (951) . 5.8 100 mL . 6. . . . KS H ISO 707/IDF 50 . 7. 7.1 . 95 (5.7) , 95 5 . . 95 5 . . A
8、LP 10 mU/L . 7.2 7.2.1 . . 7.2.2 . 7.2.3 ALP ALP ( “A/D Test” . “START” . . (3024) . A/D . 8.4.1.1.4 (8.4.1.1.2) . (60212) . . 602 (potentiometer) . 8.4.1.1.5 A/D (4.3) . A/D 650 FLU . 1 200 FLU . 8.4.1.2 , (Phospha Check-NTM) (3) (3) 02062 Norwood, 2 Technology Way Advanced Instruments . , ISO IDF
9、. , 3 ( , , Phospha Check NTM) 75 L 2 mL . 10 , Phospha Check NTM 40 . 8.4.2 8.4.2.1 . 7.1 . 10 mU/L . 10 mU/L 8.4.1.2 . H ISO 11816 1： 2006 6 8.4.2.2 (3) . (7.1) . 8.4.3 ALP , (5.3.2) (7.2.2 7.2.3) 0.075 mL 38 (5.4) 10 A(4.4.1) 2.0 mL . Fluorophos (5.1) 8.2 . 20 mU/L . . 8.4.4 (7.2.2 7.2.3) , 63 (5
10、.7) . 63 30 . 8.3 . . 9. 9.1 Fluorophos (5.1) . . (5.1) 0 A(4.4.1) B(4.4.2) C (4.4.3) . (1) (K) . K =42BCFF + (1) K： FC： A(4.4.1) C (4.4.3) (8.2 ) FB： A(4.4.1) B (4.4.2) (8.2 ) 9.2 (2) , AP . AP= fVKcFBav (2) AP： (7.2.2 7.2.3) (mU/L) Fav： 2 3 A(8.2 ) (8.3) cB： B(4.4.2) Fluoroyellow ( 2 mL mol) f： ,
11、(7.2.2)： f = 1106 (7.2.3)： f = 1108 (7.2.3)： ( f = ft106) ft f = 1106 . H ISO 11816 1： 2006 7 V： (mL) 9.3 mU . 10. 10.1 A . . 10.2 1 r , 5 % . 1 ( r ) mU/L 20 40 100 350 500 21.50 22.10 89.60 93.30 10.43 16.26 33.67 96.82 99.76 8.63 7.98 26.20 42.83 28.56 10.3 2 R , 5 % . 2 ( R ) mU/L 20 40 100 350
12、500 31.80 51.00 136.40 211.10 16.63 20.34 46.63 170.24 233.10 10.69 20.55 28.71 127.89 87.51 11. . a) b) ( ) c) d) e) H ISO 11816 1： 2006 8 A() 7 ( , , , , , ) 13 4 KS A ISO 5725 1 KS A ISO 5725 2 . 2004 3 . A.1 A.2 KS A ISO 5725 1 KS A ISO 5725 2 . , . 5 . A.1 (RSDr：%) mU/L 20 40 100 350 500 (whole
13、) 16.20 22.51 6.79 4.60 8.37 13.15 13.19 7.53 12.72 2.97 13.08 7.94 5.32 5.84 (1) 12.86 5.12 4.68 3.33 ( ) 18.66 7.69 8.89 6.48 11.68 12.04 10.68 7.86 5.69 13.81 5.94 7.29 3.65 1.74 (1) . A.2 (RSDR：%) mU/L 20 40 100 350 500 16.20 25.44 15.96 6.60 17.29 24.30 17.19 16.45 16.86 9.79 27.21 19.01 9.81 1
14、2.37 (1) 22.13 11.26 10.47 9.50 ( ) 27.60 17.69 13.53 14.66 18.62 15.07 14.79 13.82 13.30 17.11 15.31 7.98 10.91 5.33 (1) . 20 mU/L RSDr( “r”) RSDR( “R”) . Fluorophos ALP 10 mU/L . 10 mU/L . H ISO 11816 1： 2006 9 B() 1 KS H ISO 707 2 KS A ISO 5725 1： 2002 ( ) 1： 3 KS A ISO 5725 2： 2002 ( ) 2： 4 Inte
15、rnational Union of Biochemistry Nomenclature. JAMA, 260(73), 1988 5 HARDING, F. and GARRY, E. Collaborative evaluation of a fluorimetric method for measuring alkaline phosphatase activity in cow, sheep and goat milk. J. Food Prot., 68(5), 2005, pp. 10471053 1 ： 153787 1 92 3(13) (02)26240114 (02)2624 01489 http:/ KSKSKSSKSKS KSKS SKS KSKS SKSKS KSKSKSKorean Agency for Technology and Standards http:/www.kats.go.kr KS H ISO 11816 1： 2006 Milk and milk productsDeterminationof alkaline phosphatase activityPart 1：Fluorimetric method formilk and milk based drinksICS 67.100.10
