1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO 17616 KS I ISO 17616:2009 2009 12 18 http:/www.kats.go.krKS I ISO 17616:2009 : ( ) ( ) () ( ) : (ISO/TC190) () ( ) () : (http:/www.standard.go.kr) ： ：2009 12 18 2009-0831 ： ：(ISO/TC190) ( 02-509-7266) (http:/www.kats.go.kr). 10 5 , . KS I ISO 17616:
2、2009 Soil quality Guidance on the choice and evaluation of bioassays for ecotoxicological characterization of soils and soil materials 2008 1 ISO 17616, Soil quality Guidance on the choice and evaluation of bioassays for ecotoxicological characterization of soils and soil materials , . 1 , , , . . .
3、 , . (effect value) , . 2 . . ( ) . KS I ISO 6341, (Daphinia magna Straus) KS I ISO 8692, KS I ISO 11074, KS I ISO 11267, Collembola(Flosomia candida) KS I ISO 11268 1, ( ) KS I ISO 11268 2, (Eisenia fetida) 2： KS I ISO 11269 2, ( ) KS I ISO 11348( ), ( ) KS I ISO 13829, umu KS I ISO 17616:2009 2 KS
4、 I ISO 15176, KS I ISO 15685, KS I ISO 15799, KS I ISO 15952, (Helicidae) KS I ISO 16240, / (AMES ) KS I ISO 16387, Enchytraeidae(Enchytraeus sp.) KS I ISO 17155, KS I ISO 17402, KS I ISO 20079, (Lemna minor) KS I ISO 20963, (Oxythyrea funesta) KS I ISO 22030, ISO 20665, Water quality Determination
5、of chronic toxicity to Ceriodaphnia dubia ISO 20666, Water quality Determination of the chronic toxicity to Brachionus calyciflorus in 48 h 3 . 3.1 3.1.1 , , TRIAD ( ) , , TRIAD ( , ), ( , ), ( , ) . 3.1.2 3.1.3 LID (lowest-ineffective-dilution value) (： 20 % ) LID 8 1： 8 . 1 . Umu-test KS I ISO 138
6、29 “DLi ” . KS I ISO 16240 “decisive Dmin ” . 2 A . 3.2 KS I ISO 17616:2009 3 3.2.1 , , , KS I ISO 11074 3.2.2 (soil material) , , , KS I ISO 17402 3.2.3 3.3 3.3.1 , , , (biocenoses) / KS I ISO 15799 3.3.2 / KS I ISO 15799： 2003 3.3.3 (contaminant) KS I ISO 15176 (3.3.4) . 3.3.4 (pollutant) , (3.3.3
7、) KS I ISO 15176 . KS I ISO 11074 3.4 3.4.1 KS I ISO 15176 KS I ISO 17616:2009 4 4 (test battery) . . , ( , , ) . . . . . . (off-site, on-site, in-situ) , , (： , , , ) , , . / , ： ( , ) 5 , , 5.1 . a) (5.2) b) (： ) (5.1) c) (5.2) 5.2 5.2.1 , ( 1 ) ( 2 ) (KS I ISO 15799 ) . 1 . . KS I ISO 17616:2009
8、5 , . , . 1 5.2.2 / , , ( 1 ) . ( 1 KS I ISO 15799 A ). 1 . , / . LID , . ( ) . , . / ( ) . . . . KS I ISO 17616:2009 6 1 LIDa, b b Vibrio fischeri KS I ISO 11348 LID 8 20 % 1 Daphnia magna KS I ISO 6341 LID 4 20 % Lemna minor KS I ISO 20079 25 % Pseudokirchneriellasubcaptitata KS I ISO 8692 LID 4 2
9、5 % Ceriodaphnia dubia KS I ISO 20665 20 % 30 % 1 Brachionus calicyflorus KS I ISO 20666 20 % 30 % Salmonella choleraesuis subsp. Choleraesuis KS I ISO 13829 umuC DLi3 Salmonella choleraesuis subsp. Choleraesuis KS I ISO 13829 1： 15umuC DLi3 Salmonella typhimurium TA 98 and TA 100 KS I ISO 16240 Dec
10、isive Dmin value2 aLID L/S 2： 1 . b . False positive . . . , . 5.2.3 , ( 2 ) . Umu-test 15 . . , , Ames-test . Salmonella typhimurium TA 98 TA 100 . KS I ISO 17616:2009 7 2 1 KS I ISO 11269 2 30 %, Gm SD 0.9Gcalc Gm： SD ： Gm Gcalc： (Gsoil Gcontrol)2 1 bEisenia fetida KS I ISO 11268 1 20 % 1 Oxythyre
11、a funesta KS I ISO 20963 20 % KS I I SO 17155 (QR) 0.3 QR0.2 0.3a 20 htpeak max 50 h (： ) . , 25 % . KS I ISO 15685 10 % . Am SD 0.9Acalc Am： Acalc： (Asoil Acontrol)21 a (： ) . , 25 % . KS I ISO 17616:2009 8 2 ( ) 1 Brassica rapa Avena sativa KS I ISO 22030 30 %, 50 % Eisenia fetida KS I ISO 11268 2
12、 50 % bFolsomia candida KS I ISO 11267 50 % bEnchytraeus sp. KS I ISO 16387 50 % b 1 Helix aspersa KS I ISO 15952 50 % ba . b . False positive . . 5.2.4 / ( ) KS I ISO 15799 A . qualification 2 . , , . , , pH . , . KS I ISO 17616:2009 9 A ( ) , . “ ” . “ (toxic)” (LID： , 20 %, 10 % . ： 2 ). , “ (non
13、toxic) . , (Vibrio fischeri LID8, Daphnia magna LID 4, umu-test DLi3, Salmonella microsome test decisive Dminvalue2) . “toxic” . , “Lufa 2.3” Vibrio fischeri LID 8 . 8 . 6 7 . KS I ISO 17616:2009 10 1 DECHEMA, 2001. Biologische Testverfahren fr Boden und Bodenmaterial. Ed. ：DECHEMAArbeitsgruppe Val
14、idierung biologischer Testmethoden fr Bden Wolfgang Dott.Frankfurt am Main, 61 pp. 2 HEIDEN, S., ERB, R., DOTT, W., EISENTRGER, A. (Ed.), 2001. Toxikologische Beurteilung von Bden Leistungsfhigkeit biologischer Testverfahren. Spektrum Akademischer Verlag, Heidelberg, Berlin, 249 pp. 3 EHRLICHMANN, H
15、 DOTT, W., EISENTRGER, A. (2000). Assessment of the water-extractable genetoxic potential of soil samples from contaminated sites. Ecotoxicology and Environmental Safety 46, pp. 73 80 4 EISENTRGER, A., RILA, J. P., HUND RINKE, K., RMBKE, J. (2004). Proposal of a testing strategy and assessment cri
16、teria for the ecotoxicological assessment of soil or soil materials. Journal of Soils and Sediments 4 (2), pp. 123 128 5 HUND RINKE, K., KRDEL, W., HENNECKE, D., ACHAZI, R., WARNECKE, D., WILKE, B. M., WINKEL, B., HEIDEN, St. (2002a)： Bioassays for the Ecotoxicological and Genotoxicological Assessme
17、nt of contaminated soils. Part II： Assessment of the habitat function of soils Tests with soil microflora and fauna. JSS J. Soils & Sediments 2 (2), pp. 83 90 6 HUND RINKE, K., KRDEL, W., HEIDEN, St., ERB, R. (eds.) (2002). kotoxikologische Testbatterien Ergebnisse eines DBU-gefrderten Ringtests. In
18、itiativen zum Umweltschutz 45. Erich Schmidt Verlag 7 MAXAM, G., RILA, J. P., DOTT, W., EISENTRGER, A. (2000). Use of Bioassays of WaterExtractable Ecotoxic Potential of Soils. Ecotoxicology and Environmental Safety 45, pp. 240 246 8 RILA, J. P, EISENTRGER, A. (2003). Application of bioassays for ri
19、sk characterisation and remediation control of soils polluted with nitroaromatics and PAHs. Water, Air, and Soil Pollution 148, pp. 223 242 9 RUTGERS, M., POSTMA, J. and FABER, J. (2000). Basic approach for site specific, land-use specific ecological risk assessment of soil contamination in practice
20、 Program Integrated Soil Res. Report No. 29, PGBO, Wageningen 10 KS I ISO 21268 1, , 1： / ( ) 2 L/kg 11 KS I ISO 21268 2, , 2： / ( ) 10 L/kg 12 HUND RINKE, K., KRDEL, W., HENNECKE, D., EISENTRGER, A., HEIDEN, St. (2002). Bioassays for the Ecotoxicological and Genotoxicological Assessment of contami
21、nated soils. Part I： Ecotoxicological and genotoxicological tests with aqueous soil extracts. JSS J. Soils & Sediments 2 (1), pp. 43 50 13 RILA, J.P., EISENTRGER, A. (2007). Influence of laboratory storage on the organic contaminant content and water-extractable ecotoxicological potential of soil sa
22、mples. Archives of Environmental Contamination and Toxicology, 52, 1, pp. 22 31 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ Korean Agency for Technology and Standards http:/www.kats.go.kr KS I ISO 17616:2009KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Soil quality Guidance on the choice and evaluation of bioassays for ecotoxicological characterization of soils and soil materials ICS 13.080.05
