1、 KSKSKSKS SKSKSKS KSKSKS SKSKS KSKS SKS KS 2006 10 27 http:/www.kats.go.krKS I ISO 23753 1 1 ： Triphenyltetrazolium chloride(TTC) KS I ISO 23753 1： 2006 (2011 ) I ISO 23753 1： 2006 : ( ) ( ) KIST ( ) : (http:/www.standard.go.kr) ： ：2006 10 27 ：2011 12 26 2011-0622 ： ： ( 02- 509-7270) (http:/www.kats
2、.go.kr). 10 5 , . ICS 13.080.30 KS I ISO 1 ： Triphenyltetrazolium chloride(TTC) 23753 1： 2006(2011 ) Soil quality Determination of dehydrogenase activity in soils Part 1： Method using triphenyltetrazolium chloride(TTC) 2005 1 ISO 23753 1 Soil quality Determination of dehydrogenase activity in soils
3、Part 1： Method using triphenyltetrazolium chloride(TTC) . 1. 2,3,5 triphenyltetrazolium chloride(TTC) . ( ) (L, F, H ) ( ) . 2. . . KS M ISO 10381 6 ( , ) KS M ISO 10390 pH KS M ISO 11259 KS M ISO 11465 ( ) 3. TTC 25 16 . triphenylformazan(TPF) 485 nm . 1. 3 . 2. ( , ). 1 , . ( ) , 2. (II) TTC . 3.
4、( L, F, H , ) . (XVP) (XLP) . 4. I ISO 23753 1： 2006 2 4.1 KS M ISO 10381 6 . , 4 3 . KS M ISO 11465 . , . 4.2 c(HCl) 1 mol/L 4.3 (Tris) c 0.1 mol/L tris(hydroxymethyl)aminomethane 12.11 g 600 mL pH . a) (pH 6 ) 7.8 b) (pH 6 7) 7.6 c) (pH 7 ) 7.4 1 mol/L pH 1 000 mL . pH KS M ISO 10390 . 4.4 (TTC) T
5、TC 0.1 2 % . KS M ISO 11259 . 2,3,5 triphenyltetrazolium chloride (4.3). 4 1 . . a) , ： 0.1 % 0.5 % TTC b) , ： 0.6 % 1.0 % TTC c) ： 1.0 % 2.0 % TTC 4.5 4.6 Triphenylformazan(TPF) 4.6.1 TPF TPF 1 000 mg (4.5) 100 mL . 4.6.2 TPF (4.6.1) 1.0 mL (4.5) 100 mL . 4.6.3 TPF 4.6.2 0 mL, 1.0 mL, 2.0 mL, 5.0 m
6、L, 10.0 mL 5 (4.5) 30 mL . TPF 0 g/mL, 3.33 g/mL, 6.67 g/mL, 16.7 g/mL, 33.3 g/mL . TTC TPF . 5. 5.1 5.2 pH 5.3 2 cm 30 mL ( 5 g, 6. ). 5.4 (251) . 5.5 , , , 5.6 5.7 (90 100 ). 6. 4 (5.3) 5 g . 3 ( I ISO 23753 1： 2006 3 ) (4.4) 5 mL . 1 (4.3) 5 mL . 25 16 . triphenylformazan , (4.5) 25 mL 2 . , 1 2
7、. . 1 485 nm . 1 1： 1 . TTC . , TTC . 2 g 1.5 cm, 5 g 2 cm, 10 g 2.5 cm . L, F, H , 0.5 g 1 g 2 cm 4. 7. ( ) . tmVa=DM100)(bscs a： (g/g) cs ： TPF (g/mL) bs ： TPF (g/mL) V： ( )(mL) m： (g) DM： KS M ISO 11465 (%) t： ( 16 )(h) 8. . a) b) (4.1) c) d) pH (pH CaCl2) e) f) I ISO 23753 1： 2006 4 1 THALMANN,
8、H.： Zur Methodik der Bestimmung der Dehydrogenaseaktivitt im Boden mittels Triphenyltetrazoliu mchlorid(TTC). Landwirtsch. Forsch. 21, 1968, pp. 243 258 2 GLATHE, H. and THALMANN, H.： Microbial activity and its relationship to fertility characteristics of some agricultural soils, with particular ref
9、erence to dehydrogenase activity Part 2. Zbl Bakt II, 124, 1970, pp. 24 36 3 HLINGER, R.： Determination of dehydrogenase activity using TTC. In： Methods in soil biology(SCHINNER, F., HLINGER, R., KANDELER, E. and MARGESIN, R. eds.). Springer Verlag, Berlin/Heidelberg/New York, 2nd edn., 1993 4 WILKE
10、, B. M.： ber Sorption und Wirkung von Blei auf die biologische Aktivitt terrestrischer Humusformen. Z. Pflanzenernaehr. Bodenk. 145, 1982, pp. 52 65 1 ： Triphenyltetrazolium chloride(TTC) 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ KSKSKSSKSKS KSKS SKS KSKS SKSKS KSKSKSKorean Agency for Technology and Standards http:/www.kats.go.kr KS I ISO 23753 1： 2006 Soil qualityDeterminati on of dehydrogenase activity in soilsPart 1：Method using triphenyltetrazolium chloride(TTC) ICS 13.080.30
