1、 KSKSKSKSKSKSKSK KSKSKS KSKSK KSKS KSK KS KS I ISO DIS 13308 (Ulva pertusa) KS I ISO DIS 13308:2011 2011 7 5 http:/www.kats.go.krKS I ISO DIS 13308:2011 : ( ) ( ) ( ) : (http:/www.standard.go.kr) ： ：2011 7 5 2011-0190 ： ： ( 02-509-7270) (http:/www.kats.go.kr). 10 5 , . KS I ISO DIS 13308:2011 i . ii
2、i iv 1 1 2 1 3 .1 4 2 5 .2 5.1 .2 5.2 .2 5.3 .3 6 3 6.1 . 3 6.2 (light-meter) 3 6.3 pH 3 6.4 (hole puncher).3 6.5 .3 6.6 .3 6.7 .3 6.8 .3 6.9 .4 7 4 7.1 4 7.2 .4 7.3 .4 7.4 .4 7.5 4 7.6 .5 7.7 .5 7.8 .6 7.9 6 8 8 9 8 10 .8 11 .9 11.1 .9 11.2 ECx 9 11.3 ECx 9 12 .9 A( ) (Ulva pertusa) .11 KS I ISO DI
3、S 13308:2011 ii B( ) .13 C( ) 15 D( ) 16 E( ) .17 F( ) 19 KS I ISO DIS 13308:2011 iii ISO(the International Organization for Standard) (ISO ) . ISO (ISO technical committees) . , . , ISO . , ISO (IEC) . ISO/IEC (Directives), 2 (Part 2) . . . 75 % . . ISO . ISO 2 ISO/TC 147, , SC 5, . , , , . KS I IS
4、O DIS 13308:2011 iv 2010 ISO DIS 13308 , . (Ulva pertusa) . , . , . , . , . , . . . (cell plate) , 3 , 96 . , . , . . TIE/TRE . ISO TIE/TRE . KS I ISO DIS 13308:2011 (Ulva pertusa) Water quality Toxicity test based on reproduction inhibition of the green macroalga Ulva pertusa 1 (Ulva pertusa) , . 2
5、 . . ( ) . KS I ISO 5667 16, 16 ： KS Q ISO/IEC 17025, ISO/TS 20281, Water quality Guidance on statistical interpretation of ecotoxicity data 3 . 3.1 . 3.2 35 . 3.3 , . 3.4 ECx x % KS I ISO DIS 13308:2011 2 3.5 3.6 , , 4 , ( ) ( ) . ( ) . 96 , . a) / b) 5 5.1 1 . , . 1 (OTTs 1964 ) (g/L) (NaCl) 21 (M
6、gSO47H2O) 6 (MgCl26H2O) 5 (CaCl22H2O) 1 (KCl) 0.8 (NaBr) 0.1 5.2 2 . KS I ISO DIS 13308:2011 3 2 (g/L) (NaNO3) 0.2 (NaHCO3) 0.2 (H3BO3) 0.06 (Na2SiO39H2O) 0.01 (Na2HPO4) 0.02 5.3 (Ulva pertusa) 5.3.1 1 (5.1 ). 10 g 10 L . 15 , 5 molm 2s 1 10 molm 2s 1 , 12： 12 h (3 4) . 4 . 6 . 6.1 , , 3 . 6.2 (ligh
7、t-meter), 6.3 pH 6.4 (hole puncher), 4 mm 10 mm 6.5 6.6 , 6.6.1 6.6.2 6.6.3 6.6.4 6.7 , 2.5 mL 24 6.8 , KS I ISO DIS 13308:2011 4 6.9 , 7 7.1 2 . 7.2 , . pH pH 0.3 , (HCl) (NaOH) . . pH 7.5 8.5 pH . pH pH . 7.3 5 (1.5 2.0 2 mg/L, 4 mg/L, 8 mg/L, 16 mg/L, 32 mg/L, 64 mg/L) 2 . ECX EC50 , . . , . pH .
8、 7.4 , , . 2 . , , . , 10 % 90 % . pH . 25 45 . 7.5 KS I ISO DIS 13308:2011 5 ( 4 ). . 7.6 24 4 2 mL . 4 2 mL . 1 . . . , (ISO/TS 20281 ). 7.7 . 80 molm 2s 1 100 molm 2s 1 12： 12 h LD 35 15 96 . 0.2 m 4 6 . , 4 000 lx 5 000 lx . 25 40 . 15 2 . 2 . . , 10 % . 3 . KS I ISO DIS 13308:2011 6 3 (EC50) (U
9、lva pertusa) 96 h (152) 25 35 (32 ) 4 000 lx 5 000 lx (80 molm 2s 1 100 molm 2s 1) 12 h , 12 h 24 ( 15 mm) 2.5 mL 4 ( 4 mm) 1 ( 10 mm) 5 + 4 50 mL 100 mL 80 % 7.8 96 . 7.9 . . , .: 0 % (- ), ( - ) 1/4 25 %, 1/4 1/2 50 %, 1/2 3/4 75 % 3/4 100 % . 16 . KS I ISO DIS 13308:2011 7 1 . . 2 16 . . . . . %
10、KS I ISO DIS 13308:2011 8 (creamier white) (ivory-white) , . 8 (CAS No.7440508) . 9 7 . 2 1 . (CAS No 7440508, JUNSEI, Japan) . 4 A . 4 EC(r)50 CV N mg/L % 7 11 0 0.097 0.033 34.51 7 4 3 0.117 0.023 19.34 10 80 % . . , (CAS No. 7440508) EC50 0.0980.033( 2 ) , 0.1060.008 ( 2 ) . 10 % KS I ISO DIS 133
11、08:2011 9 EC50 2 . 11 11.1 , . . , , . ECx . 11.2 ECx , . ECx . P 0.400 3 0.102 0.067 0.124 4 0.102 0.094 0.109 5 0.400 6 0.400 7 0.150 0.086 0.170 0.117 0.023 CV(%) 19.34 0.400 mg/L . KS I ISO DIS 13308:2011 13 B ( ) B.1 (Con： , T1 5： ) B.2 (R1 4) 16 (Con： , T1 5： ) KS I ISO DIS 13308:2011 14 B.3 (
12、R1 4) (Con： , T1 5： ). . . B.4 (R1 4) (Con： , T1 5： ). . . KS I ISO DIS 13308:2011 15 C ( ) . 4 (4 = 97 , 16 = 97, 62, 73, 75 ) 30 25 . . KS I ISO DIS 13308:2011 16 D ( ) g/100 mL 0 3.4 2 3.2 4 3.0 6 2.8 8 2.6 10 2.4 12 2.2 14 2.0 16 1.8 18 1.6 20 1.4 22 1.2 24 1.0 26 0.8 28 0.6 30 0.4 32 0.2 34 0.0
13、 KS I ISO DIS 13308:2011 17 E ( ) : (Verlaque 2002) (Stegenga, Kaaremans & Simons 2007) (Lpez 2007) : (Hayden & Waaland 2004) : (Silva, Basson & Moe 1996) (Silva, Basson & Moe 1996) (Silva, Basson & Moe 1996) : (Silva, Basson & Moe 1996) : KS I ISO DIS 13308:2011 18 (Tseng 1984, Jin et al. 2005) (Ya
14、bu & Tokida 1960, Hayden & Waaland 2004) (Lee & Kang 2001, Kim et al. 2004, Han et al. 2008) (Huang 2000) : (Silva, Basson & Moe 1996) (Silva, Meez & Moe 1987) (Ruangchuay, Lueangthuwapranit & Pianthumdee 2007) (Teo & Wee 1983, Silva, Basson & Moe 1996) : (Barr 2007) KS I ISO DIS 13308:2011 19 F ( )
15、 . Ulva . 7 . 2 1 . (CAS No 7440508, JUNSEI, Japan) . F.1 F. Ulva pertusa EC(r)50 CV 7 11 0 0.097 0.033 34.51 (16 grid-film) 7 4 3 0.117 0.023 19.34 ( Ulva pertusa) 153787 1 92 3(13) (02)26240114 (02)262401489 http:/ Korean Agency for Technology and Standards http:/www.kats.go.kr KS I ISO DIS 13308:2011KSKSKS SKSKS KSKS SKS KS SKS KSKS SKSKS KSKSKS Water qualityToxicity test based on reproduction inhibition of the green macroalga Ulva pertusa ICS 13.060.70
