2019高考生物二轮复习专题七鸭第Ⅱ课时基因工程与克隆技术课件20190220330.ppt

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第课时 基因工程与克隆技术(选修),串点成面自成体系,点点落实(一) 基因工程,一过基础,具体操作,方法,已知脱氧核苷酸序列的目的基因DNA,经过变性、复性和延伸,形成大量目的基因,PCR技术,以mRNA为模板,在_作用下人工合成,逆转 录法,已知核苷酸序列的_,直接利用DNA合成仪用化学方法合成,不需要模板,化学 合成,人工合成,从自然界已有的物种中分离,如从_中获取,直接分离,微生物细胞,动物细胞,植物细胞,原核细胞,_,受精卵、体细胞,受体细胞,_法 (用Ca2处理),_技术,_法、基因枪法、_法,常用方法,图解,说明,过程,72 左右时,_有最大活性,可使DNA新链由5端向3端延伸,_,温度下降到_左右,两种引物通过碱基互补配对与两条单链DNA结合,复性,当温度上升到_以上时,双链DNA解聚为单链,变性,二过易错,三过高考,03,点点落实(二) 克隆技术,一过基础,二过易错,三过高考,03,提能增分检测,课堂巩固落实,谢,观,看,THANK YOU FOR WATCHING,谢,

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