1、 Collection of SANS standards in electronic format (PDF) 1. Copyright This standard is available to staff members of companies that have subscribed to the complete collection of SANS standards in accordance with a formal copyright agreement. This document may reside on a CENTRAL FILE SERVER or INTRA
2、NET SYSTEM only. Unless specific permission has been granted, this document MAY NOT be sent or given to staff members from other companies or organizations. Doing so would constitute a VIOLATION of SABS copyright rules. 2. Indemnity The South African Bureau of Standards accepts no liability for any
3、damage whatsoever than may result from the use of this material or the information contain therein, irrespective of the cause and quantum thereof. ISBN 978-0-626-22078-5 SANS 5461:2008Edition 4SOUTH AFRICAN NATIONAL STANDARD Pesticides Rearing and handling of the rice weevil (Sitophilus oryzae (L.)
4、Published by SABS Standards Division 1 Dr Lategan Road Groenkloof Private Bag X191 Pretoria 0001Tel: +27 12 428 7911 Fax: +27 12 344 1568 www.sabs.co.za SABS SANS 5461:2008 Edition 4 Table of changes Change No. Date Scope Foreword This South African standard was approved by National Committee SABS S
5、C 1028F, Pesticides Laboratory facilities and rearing and handling of test animals, in accordance with procedures of the SABS Standards Division, in compliance with annex 3 of the WTO/TBT agreement. This document was published in December 2008. This document supersedes SABS SM 461:1977 (second revis
6、ion). SANS 5461:2008 Edition 4 1 Pesticides Rearing and handling of the rice weevil (Sitophilus oryzae (L.) 1 Scope This standard specifies a method for the rearing and handling of the rice weevil (Sitophilus oryzae (L.). 2 Normative references The following referenced documents are indispensable fo
7、r the application of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. Information on currently valid national and international standards can be obtained from the SABS Standa
8、rds Division. SANS 5808, Pesticides General conditions and equipment for the rearing and handling of test insects. 3 Rearing conditions Maintain the rearing conditions prescribed in SANS 5808. 4 Rearing equipment 4.1 Oviposition and rearing containers and lids, type A rearing containers and type A r
9、earing container lids as described in SANS 5808. 4.2 Sieves, two type A sieves as described in SANS 5808 of nominal aperture sizes 4,0 mm and 850 m, with the former placed on top of the latter. 5 Rearing medium Approximately 4,5 kg of maize, heat-sterilized at a temperature of 60 C 5 C for 30 min. 6
10、 Procedure 6.1 Place about 20 000 (160 mL) of freshly-emerged adult rice weevils on top of the rearing medium (see clause 5) in a rearing container (see 4.1). 6.2 After 14 d sieve the entire contents of the rearing container gently through both sieves (see 4.2) to separate the infested rearing mediu
11、m, the adults, and the debris. SANS 5461:2008 Edition 4 2 6.3 Return the infested rearing medium to the rearing container. Weevils will emerge after four weeks to eight weeks. 6.4 Rear new colonies by repeating the procedure in 6.1 to 6.3 (inclusive), but using 80 mL of the adults retained in 6.2 and 80 mL of the weevils obtained in 6.3 for each colony. Destroy the excess adults from 6.2. SABS
