1、Designation: E 237 02Standard Specification forLaboratory Glass Microvolumetric Vessels (VolumetricFlasks and Centrifuge Tubes)1This standard is issued under the fixed designation E 237; the number immediately following the designation indicates the year oforiginal adoption or, in the case of revisi
2、on, the year of last revision. A number in parentheses indicates the year of last reapproval. Asuperscript epsilon (e) indicates an editorial change since the last revision or reapproval.1. Scope1.1 This specification covers volumetric flasks and fourtypes of centrifuge tubes, widely used in microch
3、emistry.NOTE 1Specifications for several items listed below were developedby the Committee on Microchemical Apparatus, Division of AnalyticalChemistry, American Chemical Society.21.2 Product with a stated capacity not listed in this standardmay be specified Class A tolerance when product conforms to
4、the tolerance range of the next smaller volumetric standardproduct listed in Table 1.2. Referenced Documents2.1 ASTM Standards:E 438 Specification for Glasses in Laboratory Apparatus3E 542 Practice for Calibration of Laboratory VolumetricApparatus3E 671 Specification for Maximum Permissible ThermalR
5、esidual Stress in Annealed Glass Laboratory Apparatus3E 694 Specification for Laboratory Glass Volumetric Appa-ratus3E 920 Specification for Commercially Packaged LaboratoryApparatus3E 921 Specification for Export Packaged Laboratory Appa-ratus3E 1133 Practice for Performance Testing of Packaged Lab
6、o-ratory Apparatus for United States Government Procure-ments3E 1157 Specification for Sampling and Testing of ReusableLaboratory Apparatus33. Volumetric Flasks3.1 The volumetric flasks shall be of either Style I or StyleII of Fig. 1 and shall conform to the requirements of Fig. 1 andTable 1. Each f
7、lask shall be marked with the symbol “TC” (or“IN”), the capacity, “20C”, and a 16 mm2marking area. (Allin an optional location on the flask.)4. Centrifuge Tubes4.1 Centrifuge tubes of four types shall conform to therequirements prescribed in Tables 2-4 and Figs. 2-5. The typesshown fit into the meta
8、l shields supplied with commerciallyavailable centrifuges. Removal of the glass stoppers from thecentrifuge tubes shown in Fig. 3 and Fig. 4 is advisable beforecentrifugation. The four types of centrifuge tubes specified areas follows:4.1.1 Conical bottom, plain (Fig. 2).4.1.2 Conical bottom, stoppe
9、red (Fig. 3).4.1.3 Conical bottom, stoppered and graduated (Fig. 4).4.1.4 Cylindrical bottom, plain (Fig. 5).5. Sampling and Testing5.1 Refer to Specification E 1157.6. Calibration6.1 Flasks and graduated tubes shall be calibrated in accor-dance with Practice E 542 and conform to applicable require-
10、ments of Specification E 694. Glass shall be Type I, Class A ofSpecification E 438 and annealing shall be in accordance withSpecification E 671.7. Packaging7.1 Select from Specification E 920, Specification E 921, orPractice E 1133.8. Keywords8.1 glass, laboratory; microvolumetric; vessels1This spec
11、ification is under the jurisdiction of ASTM Committee E41 onLaboratory Apparatus and is the direct responsibility of Subcommittee E41.01 onApparatus.Current edition approved Oct. 10, 2002. Published November 2002. Originallypublished as E 237 64. Last previous edition E 237 94a(1998).2Committee on M
12、icrochemical Apparatus, Division of Analytical Chemistry,American Chemical Society, “Report on Recommended Specifications for Micro-chemical Apparatus. Volumetric Glassware: Flasks, Pipets, and Centrifuge Tubes,”Analytical Chemistry, Vol 28, 1956, p. 1993.3Annual Book of ASTM Standards, Vol 14.04.1C
13、opyright ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959, United States.TABLE 1 Volumetric Flask (see Fig. 1)Style Capacity, mLClass A/ Tolerance,6 mLA, ID, max/mm B, ID, ref/mm C, max/mm D, max/mm Stopper No.I 1 0.010 5.3 8.25 70 100 8II 1 0.010 5.3 95 125 8
14、I 2 0.015 6.4 10.75 70 100 8I 3 0.015 6.4 13.50 72 100 8I 4 0.020 7.3 14.00 75 100 8I 5 0.020 7.3 15.75 75 100 8I 10 0.020 8.25 18.00 110 135 9I 25 0.030 8.7 26.00 140 165 9NOTE 1The shape of the base may be either round or hexagonalFIG. 1 Volumetric Flasks (see Table 1)TABLE 2 Centrifuge Tubes, Con
15、ical Bottom, Plain (see Fig. 2)NominalCapacity,mLA,Height,mmB,ODCylindricalPortion, mmC,ODTopFinish,mmD, Lengthof Taper, mmE,ODatBottom, mm0.5 586 2 6.0 6 0.25 13.0 6 1.0 30 6 2 3.5 6 0.51616 2 8.256 0.25 13.0 6 1.0 30 6 2 3.5 6 0.5266 2 10.75 6 0.25 13.5 6 1.0 30 6 2 4.0 6 0.53746 2 10.75 6 0.25 13
16、.5 6 1.0 30 6 2 4.0 6 0.55 101 6 2 13.00 6 0.50 16.25 6 0.75 40 6 2 4.0 6 0.5TABLE 3 Centrifuge Tubes, Conical Bottom, Stoppered (see Fig. 3)NominalCapacity,mLA,Height,mmB,ODCylindricalPortion, mmC,ODTopFinish,mmD, Lengthof Taper,mmE,ODatBottom, mmF,Stopper No.0.5 66 6 2 6.06 0.25 13.0 6 1.0 30 6 2
17、3.5 6 0.5 detail G1696 2 8.25 6 0.25 13.0 6 1.0 30 6 2 3.5 6 0.5 detail H2806 2 10.75 6 0.25 13.5 6 1.0 30 6 2 4.0 6 0.5 93886 2 10.75 6 0.25 13.5 6 1.0 30 6 2 4.0 6 0.5 951156 2 13.0 6 0.50 13.5 6 1.0 40 6 2 4.0 6 0.5 9E237022TABLE 4 Volumetric Tolerances of Centrifuge Tube,Conical Bottom, Stoppere
18、d and Graduated, mLAt 0.1 60.01At 0.2 60.02At 0.3 60.03At 0.4 60.05Above 0.4 60.075NOTE 1All dimensions are in millimetres.NOTE 2Wall thickness of all sizes shall be approximately 1 mm.NOTE 3Interior of bottoms shall be rounded.FIG. 2 Centrifuge Tubes, Conical Bottom, Plain (see Table 2)NOTE 1All di
19、mensions are in millimetres.NOTE 2Wall thickness of all sizes shall be approximately 1 mm.NOTE 3Interior of bottoms shall be rounded.FIG. 3 Centrifuge Tubes, Conical Bottom, Stoppered (see Table 3)E237023ASTM International takes no position respecting the validity of any patent rights asserted in co
20、nnection with any item mentionedin this standard. Users of this standard are expressly advised that determination of the validity of any such patent rights, and the riskof infringement of such rights, are entirely their own responsibility.This standard is subject to revision at any time by the respo
21、nsible technical committee and must be reviewed every five years andif not revised, either reapproved or withdrawn. Your comments are invited either for revision of this standard or for additional standardsand should be addressed to ASTM International Headquarters. Your comments will receive careful
22、 consideration at a meeting of theresponsible technical committee, which you may attend. If you feel that your comments have not received a fair hearing you shouldmake your views known to the ASTM Committee on Standards, at the address shown below.This standard is copyrighted by ASTM International,
23、100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959,United States. Individual reprints (single or multiple copies) of this standard may be obtained by contacting ASTM at the aboveaddress or at 610-832-9585 (phone), 610-832-9555 (fax), or serviceastm.org (e-mail); or through the ASTM
24、 website(www.astm.org).NOTE 1All dimensions are in millimetres.NOTE 2Wall thickness shall be approximately 1 mm.NOTE 3Interior of bottom shall be rounded.FIG. 4 Centrifuge Tube, Conical Bottom, Stoppered andGraduatedNOTE 1Capacity approximately 2.6 mL.NOTE 2All dimensions are in millimetres.NOTE 3Wall thickness shall be approximately 1 mm.NOTE 4Interior of bottom shall be rounded.FIG. 5 Centrifuge Tube, Cylindrical Bottom, PlainE237024
