1、 CAN/CGSB-4.2 No. 28.4-M91 Supersedes CAN/CGSB-4.2 Method 28.4 July 1977 Extended April 1997 Reaffirmed November 2013 National Standard of Canada ICS 59.080.01 Textile test methods Resistance to micro-organisms Fungus damage test Pure culture Qualitative Copyright Canadian General Standards Board Pr
2、ovided by IHS under license with CGSBNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-The CANADIAN GENERAL STANDARDS BOARD (CGSB), under whose auspices this standard has been developed, is a government agency within Public Works and Government Services Canada. CGSB
3、is engaged in the production of voluntary standards in a wide range of subject areas through the media of standards committees and the consensus process. The standards committees are composed of representatives of relevant interests including producers, consumers and other users, retailers, governme
4、nts, educational institutions, technical, professional and trade societies, and research and testing organizations. Any given standard is developed on the consensus of views expressed by such representatives. CGSB has been accredited by the Standards Council of Canada as a national standards-develop
5、ment organization. The standards that it develops and offers as National Standards of Canada conform to the criteria and procedures established for this purpose by the Standards Council of Canada. In addition to standards it publishes as National Standards of Canada, CGSB produces standards to meet
6、particular needs, in response to requests from a variety of sources in both the public and private sectors. Both CGSB standards and CGSB national standards are developed in conformance with the policies described in the CGSB Policy and Procedures Manual for the Development and Maintenance of Standar
7、ds. CGSB standards are subject to review and revision to ensure that they keep abreast of technological progress. CGSB will initiate the review of this standard within five years of the date of publication. Suggestions for their improvement, which are always welcome, should be brought to the notice
8、of the standards committees concerned. Changes to standards are issued either as separate amendment sheets or in new editions of standards. An up-to-date listing of CGSB standards, including details on latest issues and amendments, and ordering instructions, is found in the CGSB Catalogue at our Web
9、 site www.tpsgc-pwgsc.gc.ca/ ongc-cgsb along with more information about CGSB products and services. Although the intended primary application of this standard is stated in its Scope, it is important to note that it remains the responsibility of the users of the standard to judge its suitability for
10、 their particular purpose. The testing and evaluation of a product against this standard may require the use of materials and/or equipment that could be hazardous. This document does not purport to address all the safety aspects associated with its use. Anyone using this standard has the responsibil
11、ity to consult the appropriate authorities and to establish appropriate health and safety practices in conjunction with any applicable regulatory requirements prior to its use. CGSB neither assumes nor accepts any responsibility for any injury or damage that may occur during or as the result of test
12、s, wherever performed. Attention is drawn to the possibility that some of the elements of this Canadian standard may be the subject of patent rights. CGSB shall not be held responsible for identifying any or all such patent rights. Users of this standard are expressly advised that determination of t
13、he validity of any such patent rights is entirely their own responsibility. Further information on CGSB and its services and standards may be obtained from: The Manager Standards Division Canadian General Standards Board Gatineau, Canada K1A 1G6 The Standards Council of Canada (SCC) is the coordinat
14、ing body of the Canadian standardization network, which is composed of people and organizations involved in the development, promotion and implementation of standards. Through the collaborative efforts of Canadian standardization network members, standardization is helping to advance the social and
15、economic well-being of Canada and to safeguard the health and safety of Canadians. The networks efforts are overseen by SCC. The principal objectives of SCC are to foster and promote voluntary standardization as a means of advancing the national economy, supporting sustainable development, benefitin
16、g the health, safety and welfare of workers and the public, assisting and protecting the consumer, facilitating domestic and international trade, and furthering international cooperation in relation to standardization. An important facet of the Canadian standards development system is the use of the
17、 following principles: consensus; equal access and effective participation by concerned interests; respect for diverse interests and identification of those who should be afforded access to provide the needed balance of interests; mechanism for dispute resolution; openness and transparency; open acc
18、ess by interested parties to the procedures guiding the standards development process; clarity with respect to the processes; and Canadian interest consideration as the initial basis for the development of standards. A National Standard of Canada (NSC) is a standard prepared or reviewed by an SCC-ac
19、credited SDO and approved by the SCC according to NSC approval requirements. Approval does not refer to the technical content of the standard, as this remains the responsibility of the SDO. An NSC reflects a consensus of a number of capable individuals whose collective interests provide, to the grea
20、test practicable extent, a balance of representation of general interests, producers, regulators, users (including consumers) and others with relevant interests, as may be appropriate to the subject at hand. NSCs are intended to make a significant and timely contribution to the Canadian interest. Th
21、ose who have a need to apply standards are encouraged to use NSCs. These standards are subject to periodic review. Users of NSCs are cautioned to obtain the latest edition from the SDO that publishes the standard. The responsibility for approving standards as NSCs rests with: Standards Council of Ca
22、nada 270 Albert Street, Suite 200 Ottawa, Ontario K1P 6N7, CANADA How to order Publications: by telephone 819-956-0425 or 1-800-665-2472 by fax 819-956-5740 by mail CGSB Sales Centre Gatineau, Canada K1A 1G6 in person Place du Portage Phase III, 6B1 11 Laurier Street Gatineau, Quebec by email ncr.cg
23、sb-ongctpsgc-pwgsc.gc.ca on the Web www.tpsgc-pwgsc.gc.ca/ongc-cgsb Copyright Canadian General Standards Board Provided by IHS under license with CGSBNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-NATIONAL STANDARD OF CANADA CAN/CGSB-4.2 No. 28.4-M91 Supersedes CA
24、N/CGSB-4.2 Method 28.4 July 1977 Extended April 1997 Reaffirmed November 2013 Textile test methods Resistance to micro-organisms Fungus damage test Pure culture Qualitative CETTE NORME NATIONALE DU CANADA EST DISPONIBLE EN VERSIONS FRANAISE ET ANGLAISE. Published August 1991 by the Canadian General
25、Standards Board Gatineau, Canada K1A 1G6 Minister of Supply and Services Canada 1991 No part of this publication may be reproduced in any form without the prior permission of the publisher. Prepared by the Canadian General Standards Board Approved by the Copyright Canadian General Standards Board Pr
26、ovided by IHS under license with CGSBNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-CAN/CGSB-4.2 No. 28.4-M91 CGSB 2013 All rights reserved CANADIAN GENERAL STANDARDS BOARD Committee on Textile Test Methods and Terminology (Voting membership at date of reaffirmati
27、on) General interest category Batcheller, J. University of Alberta Carrick, D. Consultant Davie, N. Consultant Liu, S. University of Manitoba Man, T.M. Consultant Tait, C. National Defence/DSSPM Producer category Adam, C. Tencate Protective Fabrics Canada Boivin, D. E.I. DuPont Co. Lawson, L. Davey
28、Textile Solutions Leblanc, J.-M. Marv Holland Apparel Ltd. Schumann, E. Lincoln Fabrics Ltd. Taylor, V. Invista (Canada) Co. Regulator category Andersson, C. Health Canada User category Bourget, S. National Defence/QETE DEntremont, E. Royal Canadian Mounted Police Izquierdo, V. Textile Technologies
29、Centre Kohli, G. Sears Canada Inc. Larsen, A.-L. Exova Group Ltd. Litva, M. Canada Border Services Agency MacLeod, J. Public Works and Government Services Canada Tebbs, C. International Drycleaners Congress Secretary (non-voting) Grabowski, M. Canadian General Standards Board Acknowledgment is made
30、for the translation of this National Standard of Canada by the Translation Bureau of Public Works and Government Services Canada. Copyright Canadian General Standards Board Provided by IHS under license with CGSBNot for ResaleNo reproduction or networking permitted without license from IHS-,-,- CGSB
31、 2013 All rights reserved i CAN/CGSB-4.2 No. 28.4-M91 Supersedes CAN/CGSB-4.2 Method 28.4 July 1977 Extended April 1997 Reaffirmed November 2013 Preface to the National Standard of Canada This National Standard of Canada has been extended and reaffirmed by the CGSB Committee on Textile Test Methods
32、and Terminology. It is published without editorial changes. Copyright Canadian General Standards Board Provided by IHS under license with CGSBNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-Supersedes CAN/CGSB-4.2Method 28.4July 1977ExtendedApril 1997ReaffirmedNove
33、mber 2013Copyright Canadian General Standards Board Provided by IHS under license with CGSBNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-Autoclave: an autoclave capable of being operated at a steam pressure of 103 to 117 kPa (gauge) and an exhaust temperature of
34、121 to 123“C, these being the sterilizing conditions used throughout. Incubator: an incubator room or cabinet maintained at 30 f 2C. Petri dishes: 100 mm in diameter. Leaching Apparatus This shall consist of a reservoir of water at 24 f 3C from which water is passed to a suitable vessel containing t
35、he fabric to be leached. The sue of this vessel will depend on the sue of the sample to be leached. The rate of flow of water through the vessel should be such as to provide approximately four changes of water per hour. The water used must not contain any material that may react with any of the cons
36、tituents of the sample being tested (Note 2). Wherever possible, distilled water or demineralized water shall be used. The pH of the water shall not be less than 5.0 nor greater than 8.0. Low power binocular microscope (SOX). TEST SPECIMENS Fabrics - A minimum of five specimens (40 mm diameter circl
37、es or 50 x 50 mm squares) are taken from widely distributed areas of the sample (Note 3). Yarns - A minimum of five representative specimens, each approximately 500 mm long, are taken and wound into loops approximately 50 mm long. The specimens are leached for 24 h (Note 4). PROCEDURE Sterilize the
38、required number of 100 mm Petri dishes under the conditions specified in par. 3.4. Sterilize the salts-agar culture medium, pour it into the sterile Petri dishes, and allow to stand until the agar has hardened. Using a sterile loop of nichrome, tungsten or platinum wire, add scrapings from a ripe fr
39、uiting culture of the organism, 10 to 14 d old, and sufficient to cover a 150 mm Petri dish, to an Erlenmeyer flask containing 100 mL of sterile water. Shake the suspension of spore clusters in the flask with sterile glass beads until the spores are in suspension. In order to maintain a uniform susp
40、ension, the addition of a surface-active agent is recommended (Note 5). Inoculate the surface of the agar with approximately 1.0 mL of spore suspension. Place the specimen on the seeded agar. Using a pipette, inoculate the specimen with 0.2 to 0.5 mL of spore suspension. Note 2: An example of the us
41、e of an unsuitable type of water is to employ a water containing alkaline impurities to leach a sample carrying a phenolic type fungicide; depending on the degree of alkalinity, solubilization of the fungicide by the alkali in the water will occur through the formation of a soluble phenate, and the
42、loss thus occasioned would not represent a loss through water solubility of the original phenolic compound. Note 3: For specimens whose surfaces are not plane (e.g., rubber-lined fire hose), it is recommended that the size of the specimens be reduced to a point at which they are reasonably flat so a
43、s to maintain good contact with the agar surface in the inoculation and incubation procedure. Note 4: To facilitate wetting by the spore suspension, it is desirable that the inoculation of the specimens (section 5) be proceeded with immediately following leaching, before the specimens have become dr
44、y. Where drying has occurred, the specimens should be dampened by being thoroughly sprayed with distilled water and allowed to stand for 1 to 2 h, flat, on a non- absorbent surface in a closed container, to avoid dessication. Note 5: The product Aerosol OT (sodium di-octyl sulphosuccinate) at a conc
45、entration of 0.05% is suitable. Copyright Canadian General Standards Board Provided by IHS under license with CGSBNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-5.5 In order to ensure that the organism is capable of satisfactory performance, carry out a viability
46、check using sterile filter paper (e.g., Whatman No. 2) approximately 50 mm in diameter, inoculated and incubated as above. 5.6 Incubate the Petri dishes and contents for two weeks at 30 + 2C. Examine the specimen for fungal growth with a low power binocular microscope. Keep the specimens under obser
47、vation during that period, and note the presence or absence of growth, as well as the presence or absence of fruiting bodies. 5.7 If by the end of the incubation period there has been appreciable growth of the test organism, continue the test for a further period of one week, and note any further gr
48、owth occurring in this period. 6. REPORT Report the following: 6.1 The presence or absence of growth in the first two-week period, together with information on such further growth that may have occurred (par. 5.7). 6.2 The number of this method: CANJCGSB-4.2 No. 28.4-M91. Copyright Canadian General Standards Board Provided by IHS under license with CGSBNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-
