1、 September 2006DIN EN 14735 Corrigendum 1 ICS 13.030.01 Characterization of waste Preparation of waste samples for ecotoxicity tests Corrigendum 1 to English version of DIN EN 14735:2005-11 Charakterisierung von Abfllen Herstellung von Abfallproben fr kotoxikologische Untersuchungen Berichtigung 1 z
2、ur englischen Fassung DIN EN 14735:2005-11 Document comprises 5 pages No part of this standard may be reproduced without prior permission of DIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany, has the exclusive right of sale for German Standards (DIN-Normen). E
3、nglish price group 99 www.din.de www.beuth.de !,qC9“03.07 9783222This corrigendum should be filed together with the translation of the standard to which it refers and a corresponding note made on the translation. DIN EN 14735 Corr 1:2006-09 2 This Corrigendum is based on EN 14735:2005/AC:2006, issue
4、d by CEN. In the English version of DIN EN 14735:2005-11 the following corrections are to be made: Re Introduction: Delete the numbering of clauses: 0, 0.1 and 0.2. Replace the last sentence by: This European Standard describes the necessary steps to be performed before carrying out ecotoxicity test
5、s on wastes within the context of assessment of ecotoxic properties for classification purposes. Re subclause 3.1: Replace definition 3.1 by: 3.1 dilution medium liquid or solid used for the preparation of control vessels and the preparation of test mixtures Re subclause 3.8: Replace definition 3.8
6、by: 3.8 liquid sludge sludge that has the characteristic of a liquid as specified in the definition of a liquid waste (3.9) Re clause 5 Taking of laboratory sample: Replace the NOTE by: NOTE Sampling devices are described in a Technical Report under development (prCEN/TR 15310-2). Re clause 6 Transp
7、ort: Replace the last sentence by: A transport time of less than 48 h and/or low temperature conditions (4 2) C are appropriate to maintain the properties of laboratory samples. Re subclause 7.2 Waste sample: Replace the second paragraph by: A storage time of less than two months and low temperature
8、 conditions (4 2) C are appropriate to maintain the properties of waste samples. Re clause 8 Waste characterization: Replace the NOTE by: NOTE The method described in Annex A of ISO 14238:1997 has been found to be appropriate for most kinds of waste. DIN EN 14735 Corr 1:2006-09 3 Re subclause 11.2.1
9、 Leaching procedure Replace the third paragraph by: Ecotoxicological tests usually require several litres of eluate leading to an adjustment of mass of the test portion. When a large volume of eluate is required, it is possible to perform the leaching test in a vessel of appropriate capacity into wh
10、ich the corresponding number of test portions (a dry mass of (90 5) g) are introduced or to perform parallel leaching tests at the same time with an appropriate number of vessels of 1 l capacity each (see above). In that case, all the individual eluates shall be mixed in order to obtain the test sam
11、ple. When the leaching test is performed in one vessel, the size of the vessel shall be appropriate to minimize the headspace ( 5 % of the total capacity of the vessel). DIN EN 14735 Corr 1:2006-09 4 Re B.2.4 Coleoptera Acute test: Replace B.2.4 by the following: 1. Title of the test Effects of poll
12、utants on insect larvae (Oxythyrea funesta) Determination of acute toxicity using artificial soil substrate 2. Harmonization International 3. References ISO 20963; NF X 31-260 4. Principle Determination of the percent mortality of Cetoniinae larvae during growth period after 10 days 5. Test type Sta
13、tic acute 6. Test organism Third instar larvae of the species Oxythyrea funesta Breeding stocks Age 15 days, wet mass 100 mg to 200 mg Feeding Dried and finely ground cow-dung 7. Dilution medium Artificial soil Volume 300 g (dry weight) /container 8. Test conditions Test chamber Enclosures Temperatu
14、re (26 1) C pH 6,0 0,5 Light intensity/quality Darkness Photoperiod Soil moisture 50 % of total WHC 9. Number of replicates Three (10 larvae per replicate) 10. Test duration/incubation 10 days 11. Control Artificial soil 12. Validity criteria Control: mortality 80 % 13. Reference substance ISO 20963
15、: 2,4,5 trichlorophenol, 60 LC 50 180 mg/kg NF X31-260:HgCl2, 15 mg/kg LC 50 45 mg/kg (as Hg2+) 14. Statistics Multiple t-test, u-test, regression analysis 15. Test parameter(s) Mortality, growth (optional) 16. Endpoints LC 50, NOEC (ECx) 17. Application to wastes and water extracts from wastes: lim
16、itations and comments The test organism is not sensitive to modifications of the test mixture granulometry. DIN EN 14735 Corr 1:2006-09 5 B.3.2 Daphnia magna Inhibition of reproduction Replace B.3.2 by the following: 1. Title of the test Determination of long term toxicity of substances to Daphnia m
17、agna Straus (Cladocera crustacea) 2. Harmonisation International 3. References ISO 10706; OECD guideline n211 4. Principle Inhibition of reproduction and survival of Daphnia magna 5. Test type Chronic, semi-static 6. Test organism Daphnia magna from the second to the fifth brood obtained by acyclica
18、l parthenogenesis Breeding stock Daphnia magna Straus Age of test organism 24 h Feeding Unicellular algae (Chlorella sp., Pseudokirchneriella subcapitata or Scenedesmus subspicatus). 0,1 mg carbon/animal/day to 0,2 mg carbon/animal/day 7. Dilution medium ELENDT M4 or M7 synthetic medium; ASTM recons
19、tituted hard freshwater Volume 50 ml to 100 ml 8. Test conditions Test chamber size 50 ml to 100 ml beakers Temperature Within 18 C to 22 C, variations within less than 2 C pH 6,0 to 9,0 Light intensity/quality 1 200 lux Photoperiod 16 h light 9. Number of container, number of replicates 5 concentra
20、tions 10 replicates (one animal per vessel is recommended) 10. Test duration 21 days 11. Negative control dilution ELENDT M4 or M7; ASTM 12. Validity criteria Mortality of adults or living males 20 % in the control, mean number of living offspring per parent 60 in the control, coefficient of varianc
21、e for control fecundity 20 % 13. Positive control/reference toxicant, mean EC (and CV) The Daphnid culture may be controlled using K2Cr2O7(effect on mobility) 14. Statistics Dunnett or Williams test and regression 15. Test parameter Mortality of adults, inhibition of reproduction 16. End points ECx,
22、 NOEC 17. Application to wastes and water extracts from wastes: limitations and comments This test is mainly used for pure substances. Bioavailability of metals ions can be reduced by complexation with EDTA from the test medium (ELENDT M4 or M7). Turbid and coloured samples may disturb mother and ne
23、onates behaviour and counting. When the sample is likely to interfere with the test (protozoa, micro-organisms) a filtration or centrifugation of the sample can be performed. Other limitations: volume of water extract necessary to perform the test and test duration. Freshwater organisms may be affected by test mixtures that contain high amount of salts. This may occur when testing low dilutions of water extracts (e.g. dilutions factors 1/1, 1/2, 1/4).
