1、November 2016 English price group 13No part of this translation may be reproduced without prior permission ofDIN Deutsches Institut fr Normung e. V., Berlin. Beuth Verlag GmbH, 10772 Berlin, Germany,has the exclusive right of sale for German Standards (DIN-Normen).ICS 71.100.50!%uc“2588264www.din.de
2、DIN EN 47Wood preservatives Determination of the toxic values against larvae of Hylotrupes bajulus (Linnaeus) (Laboratory method);English version EN 47:2016,English translation of DIN EN 47:2016-11Holzschutzmittel Bestimmung der Grenze der Wirksamkeit gegenber Larven von Hylotrupes bajulus (Linnaeus
3、) (Laboratoriumsverfahren);Englische Fassung EN 47:2016,Englische bersetzung von DIN EN 47:2016-11Produits de prservation du bois Dtermination du seuil defficacit contre les larves dHylotrupes bajulus (Linnaeus) (Mthode de laboratoire);Version anglaise EN 47:2016,Traduction anglaise de DIN EN 47:201
4、6-11SupersedesDIN EN 47:200506 andDIN EN 47 Corrigendum 1:200803www.beuth.deDocument comprises 25 pagesDTranslation by DIN-Sprachendienst.In case of doubt, the German-language original shall be considered authoritative.11.16 DIN EN 47:2016-11 2 A comma is used as the decimal marker. National forewor
5、d This document (EN 47:2016) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and wood-based products” (Secretariat: AFNOR, France). The responsible German body involved in its preparation was DIN-Normenausschuss Holzwirtschaft und Mbel (DIN Standards Committee Timber and Furni
6、ture), Working Committee NA 042-03-06 AA Spiegelausschuss zu CEN/TC 38 und ISO/TC 165/SC 1 Dauerhaftigkeit von Holz und Holzprodukten. Amendments This standard differs from DIN EN 47:2005-06 and DIN EN 47 Corrigendum 1:2008-03 as follows: a) the requirements for wood quality have been unified. Previ
7、ous editions DIN 52623: 1949-06 DIN 52165: 1955-02 DIN 52165-1: 1972-07 DIN EN 47: 1977-02, 1990-04, 2005-06 DIN EN 47 Corrigendum 1: 2008-03 EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 47 August 2016 ICS 71.100.50 Supersedes EN 47:2005English Version Wood preservatives - Determination of t
8、he toxic values against larvae of Hylotrupes bajulus (Linnaeus) - (Laboratory method) Produits de prservation du bois - Dtermination du seuil defficacit contre les larves dHylotrupes bajulus (Linnaeus) - (Mthode de laboratoire) Holzschutzmittel - Bestimmung der Grenze der Wirksamkeit gegenber Larven
9、 von Hylotrupes bajulus (Linnaeus) - (Laboratoriumsverfahren) This European Standard was approved by CEN on 2 January 2017. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard wit
10、hout any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the CEN-CENELEC Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other
11、 language made by translation under the responsibility of a CEN member into its own language and notified to the CEN-CENELEC Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, D
12、enmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey andUnited Kingdom. EUROPEAN COMMITT
13、EE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG CEN-CENELEC Management Centre: Avenue Marnix 17, B-1000 Brussels 2016 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 47:2016 E2 Contents Page Eur
14、opean foreword . 3 Introduction 4 1 Scope 5 2 Normative reference 5 3 Terms and definitions . 5 4 Principle . 5 5 Test materials . 6 6 Sampling . 8 7 Test specimens . 9 8 Procedure 10 9 Expression of results . 14 10 Test report 14 Annex A (informative) Example of a test report 16 Annex B (informativ
15、e) Technique for culturing Hylotrupes bajulus (Linnaeus) 18 Annex C (normative) Differentiation of heartwood and sapwood in Pinus species 21 Annex D (informative) Environmental, health and safety precautions within chemical/biological laboratory 22 Bibliography . 23 EN 47:2016 (E) DIN EN 47:2016-11
16、3 European foreword This document (EN 47:2016) has been prepared by Technical Committee CEN/TC 38 “Durability of wood and wood-based products”, the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical te
17、xt or by endorsement, at the latest by February 2017, and conflicting national standards shall be withdrawn at the latest by February 2017. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held respo
18、nsible for identifying any or all such patent rights. This document supersedes EN 47:2005. The significant technical difference between this document and EN 47:2005 is as follows: introduction of new harmonized specifications for wood quality. According to the CEN/CENELEC Internal Regulations, the n
19、ational standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, Former Yugoslav Republic of Macedonia, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia,
20、Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland, Turkey and the United Kingdom. EN 47:2016 (E) DIN EN 47:2016-11 4 Introduction This document describes a laboratory method of testing which gives a basis for the general asse
21、ssment of the effectiveness of a wood preservative against Hylotrupes bajulus by determination and comparison with different classes of larvae, of the concentration at which the product prevents their survival in totally impregnated wood of a susceptible species. In this respect it differs from the
22、method specified in EN 46-1 which is intended to determine whether a preservative applied to the surface is capable of preventing infestation of wood by these larvae. This laboratory method provides a criterion by which the value of a preservative can be assessed. In making this assessment the metho
23、ds by which the preservative may be applied should be taken into account. It is further recommended that results from this test should be supplemented by those from other appropriate tests and, above all, by comparison with practical experience. When products which are very active at very low concen
24、tration are used, it is very important to take suitable precautions to isolate and separate, as far as possible, operations involving chemical products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separate rooms, areas within rooms,
25、 extraction facilities, conditioning chambers and special training for personnel (see also Annex D for environmental, health and safety precautions). EN 47:2016 (E) DIN EN 47:2016-11 5 1 Scope This European Standard specifies a method for the determination of the toxic values of a wood preservative
26、against the larvae of Hylotrupes bajulus (Linnaeus), introduced into wood treated previously by full impregnation. This method is applicable to: water-insoluble chemicals which are being studied as active insecticides; organic formulations, as supplied or as prepared in the laboratory by dilution of
27、 concentrates; organic water-dispersible formulations as supplied or as prepared in the laboratory by dilution of concentrates; and water-soluble materials, for example salts. The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures. 2 Normative
28、 reference The following documents, in whole or in part, are normatively referenced in this document and are indispensable for its application. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) appli
29、es. EN ISO 3696, Water for analytical laboratory use Specification and test methods (ISO 3696) 3 Terms and definitions For the purposes of this document, the following terms and definitions apply. 3.1 representative sample sample having its physical or chemical characteristics identical to the volum
30、etric average characteristics of the total volume being sampled SOURCE: EN 1001-2:2005, 4.71 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to be tested) Note 1 to entry: Adapted from EN 10012:2005, 4.83. 4 Principle Impregnation of several sets of test
31、 specimens of susceptible wood species with a series of concentrations of the preservative. Introduction of Hylotrupes bajulus larvae of a given category into these test specimens and determination of their survival rate at fixed intervals of time. EN 47:2016 (E) DIN EN 47:2016-11 6 Comparison of th
32、e results with those obtained with untreated and solvent or diluent-treated control test specimens. Derivation of the toxic values of the product under test for the category of larvae in question. 5 Test materials 5.1 Biological material 5.1.1 Hylotrupes bajulus (Linnaeus) larvae Category 1 (obligat
33、ory test): larvae within a maximum of 3 days of hatching; Category 2 (optional additional test): larvae with masses in the range 50 mg to 150 mg. 5.1.2 Source of larvae The larvae shall preferably be obtained from cultures reared according to the method described in Annex B. Otherwise larvae in Cate
34、gory 2 can be taken from naturally infested wood, in which case they should be transferred into sapwood of pine and stored for at least 4 weeks under the rearing conditions specified in Annex B. Do not use the larvae in the test if they have not fed normally during this storage period. 5.1.3 Provisi
35、on of larvae Collect larvae in Category 1 from eggs laid by different females. Carefully cut out the larvae in Category 2 from the culture blocks and keep them separated from one another for 2 days to 3 days in the culturing chamber (5.3.1) to check that they are healthy. 5.1.4 Choice of larvae in C
36、ategory 2 Use only healthy larvae in the test. NOTE A healthy larva can be recognized by its ivory-white colour, its firm consistency and rounded appearance, and by the absence of wounds or bites which show up as dark marks. Healthy larvae react to the touch by vigorous movement and attempts to bite
37、. Reject any larvae which are shrunken or aged, which have recently moulted, or which are in a pre-pupal stage. 5.1.5 Number of larvae The number of larvae per treated and control test specimen shall be six of Category 1 or one of Category 2. Sort the larvae retained in Category 2 mentioned above. D
38、o not use larvae weighing more than 150 mg as they may pupate and therefore interfere with the test. For a single test, use a mixed batch of larvae of Category 1 and for Category 2, as far as possible, use larvae of similar masses. The number of larvae necessary is given in Table 1. EN 47:2016 (E) D
39、IN EN 47:2016-11 7 Table 1 Number of larvae and test specimens Type of test specimen Concentrations of preservatives Mass fraction Larvae in Category 1 Larvae in Category 2 Without radiography With radiography aNumber of test specimens Number of larvae Number of test specimens Number of larvae Numbe
40、r of test specimens Number of larvae Treated test specimens 1 5 30 10 10 7 7 _ 2 5 30 10 10 7 7 _ 3 5 30 10 10 7 7 _ 4 5 30 10 10 7 7 _ 5 5 30 10 10 7 7 etc. Untreated control test specimens 0 5 30 10 10 7 7 Solvent or diluent control testspecimens (including water) 0 5 30 10 10 7 7 Total for 5 conc
41、entrations 35 210 70 70 49 49 aThe use of radiography is only recommended in the case of tests with larvae in Category 2. 5.2 Products and reagents 5.2.1 Xylene, technical grade, mixed isomers. 5.2.2 Water, complying with grade 3 of EN ISO 3696. 5.2.3 Solvent or diluent, a volatile liquid that will
42、dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects. CAUTION Do not use benzene or other solvents which pose a health risk. 5.2.4 Cellulose or absorbent cotton wool and filter paper 5
43、.3 Apparatus 5.3.1 Culturing chamber, with air circulation, and controlled at (28 2) C and at relative humidity (70 5) %. 5.3.2 Conditioning chamber, well ventilated and controlled at (20 2) C and at relative humidity (65 5) %. NOTE The conditioning of test specimens can be carried out in the labora
44、tory work area (see 5.3.3) provided that this has the conditions specified for the conditioning chamber (see 5.3.2). 5.3.3 Laboratory work area, well ventilated, where treatment of the test specimens is carried out. EN 47:2016 (E) DIN EN 47:2016-11 8 CAUTION It is essential to follow safety procedur
45、es for handling flammable and toxic materials. Avoid excessive exposure of operators to solvents or their vapours. 5.3.4 Testing chamber, ventilated and air-conditioned, controlled at (22 2) C and at a relative humidity of (70 5) %. 5.3.5 Treatment vessels, of a material that does not react with the
46、 preservative under test, for example of glass for organic products and of polyethylene for salts containing fluorine. 5.3.6 Weights, to provide ballast for the test specimens. The weights shall not react with any materials with which they come into contact during the test. 5.3.7 Safety equipment an
47、d protective clothing, appropriate for the test product and the test solvent, to ensure the safety of the operator. 5.3.8 Vacuum vessel(s), fitted with stopcocks, capable of receiving the treatment vessels (5.3.5). 5.3.9 Vacuum pump, fitted with a pressure gauge and capable of maintaining a pressure
48、 of 700 Pa1). 5.3.10 Drying vessel(s), capable of holding sets of five test specimens (7.5), provided with a close-fitting cover and containing supports that will give minimum contact with treated test specimens to be placed on them. The vessels and supports shall be of a material that does not reac
49、t with the preservative under test, for example glass for organic compounds and polyethylene for products containing fluorine. 5.3.11 Drill and twist drills, approximately 3,0 mm to 4,5 mm in diameter, and a fine awl. In all cases, the number of bits shall be sufficient to drill holes to the size of the larvae available; in the case of larvae of Category 1, use a steel awl. 5.3.12 Ordinary laboratory equipment, including a balance c
