1、BS EN 46-1:2009ICS 71.100.50NO COPYING WITHOUT BSI PERMISSION EXCEPT AS PERMITTED BY COPYRIGHT LAWBRITISH STANDARDWood preservatives Determinationof the preventiveaction against recentlyhatched larvae ofHylotrupes bajulus(Linnaeus)Part 1: Application by surfacetreatment (laboratory method)Copyright
2、European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-This British Standardwas published under theauthority of the StandardsPolicy and StrategyCommittee on 31 December2009 BSI 2009ISBN 978 0 580
3、 66779 4Amendments/corrigenda issued since publicationDate CommentsBS EN 46-1:2009National forewordThis British Standard is the UK implementation of EN 46-1:2009. Itsupersedes BS EN 46-1:2005 which is withdrawn.The UK participation in its preparation was entrusted to TechnicalCommittee B/515, Wood p
4、reservation.A list of organizations represented on this committee can be obtained onrequest to its secretary.This publication does not purport to include all the necessary provisionsof a contract. Users are responsible for its correct application.Compliance with a British Standard cannot confer immu
5、nityfrom legal obligations.Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN 46-1:2009EUROPEAN STANDARD NORME EUROPENNE EUROPISCHE NORM EN 46-1 November 2009 ICS 71.100.50 Su
6、persedes EN 46-1:2005English Version Wood preservatives - Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) - Part 1: Application by surface treatment (laboratory method) Produits de prservation du bois - Dtermination de laction prventive contre
7、les larves rcemment closes dHylotrupes bajulus (Linnaeus) - Partie 1: Application par traitement de surface (Mthode de laboratoire) Holzschutzmittel - Bestimmung der vorbeugenden Wirkung gegenber frisch geschlpften Larven von Hylotrupes bajulus (Linnaeus) - Teil 1 : Anwendung zur Oberflchenbehandlun
8、g (Laboratoriumsverfahren) This European Standard was approved by CEN on 10 October 2009. CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date li
9、sts and bibliographical references concerning such national standards may be obtained on application to the CEN Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the re
10、sponsibility of a CEN member into its own language and notified to the CEN Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary,
11、 Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania, Slovakia, Slovenia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMIT EUROPEN DE NORMALISATION EUROPISCHES KOMITEE FR NORMUNG Management Centre: A
12、venue Marnix 17, B-1000 Brussels 2009 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 46-1:2009: ECopyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking pe
13、rmitted without license from IHS-,-,-BS EN 46-1:2009EN 46-1:2009 (E) 2 Contents Page Foreword 4Introduction .51 Scope 62 Normative reference 63 Terms and definitions .64 Principle 75 Test materials .75.1 Biological material .75.2 Products and reagents 75.3 Apparatus .76 Sampling .87 Test specimens 8
14、7.1 Species of wood.87.2 Wood quality 87.3 Provision of test specimens .97.4 Dimensions of test specimens .97.5 Number of test specimens 98 Procedure .98.1 Preparation of the test specimens .98.1.1 Conditioning of the test specimens prior to sealing98.1.2 Sealing of the transverse faces . 108.1.3 Tr
15、eatment of the test specimens . 108.1.4 Drying and conditioning of the test specimens after treatment 128.2 Exposure of the test specimens to the insects . 128.3 Conditions and duration of the test 138.4 Examination of the test specimens . 138.4.1 Examination . 138.4.2 Validity of the test . 149 Exp
16、ression of results . 149.1 Evaluation of attack 149.2 Toxic values 1410 Test report . 15Annex A (informative) Example of a test report 17Annex B (informative) Technique for culturing Hylotrupes bajulus (Linnaeus) 19B.1 General . 19B.2 Obtaining parent beetles 19B.3 Mating 19B.4 Egg-Iaying 19B.5 Hatc
17、hing of eggs . 20B.6 Larval development 20B.7 Enemies and parasites . 21Annex C (informative) Differentiation of heartwood and sapwood in Pinus species . 22C.1 Principle . 22C.2 Reagents 22C.3 Apparatus 22Copyright European Committee for Standardization Provided by IHS under license with CENNot for
18、ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN 46-1:2009EN 46-1:2009 (E) 3 C.4 Procedure . 22Annex D (informative) Environmental, health and safety precautions within chemical/biological laboratory . 23Annex E (normative) Slow acting and deferred effect formulations
19、 Extending the test duration 24Bibliography 26Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN 46-1:2009EN 46-1:2009 (E) 4 Foreword This document (EN 46-1:2009) has been pre
20、pared by Technical Committee CEN/TC 38 “Durability of wood and wood-based products”, the secretariat of which is held by AFNOR. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by May 2010, and confli
21、cting national standards shall be withdrawn at the latest by May 2010. Attention is drawn to the possibility that some of the elements of this document may be the subject of patent rights. CEN and/or CENELEC shall not be held responsible for identifying any or all such patent rights. This document s
22、upersedes EN 46-1:2005. The standard EN 46 is composed of two parts: EN 46-1, Wood preservatives Determination of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 1: Application by surface treatment (laboratory method) EN 46-2, Wood preservatives Determinat
23、ion of the preventive action against recently hatched larvae of Hylotrupes bajulus (Linnaeus) Part 2: Ovicidal effect (laboratory method) EN 46 consists of two parts to enable preventive action of wood preservatives, against recently hatched larvae of Hylotrupes bajulus, which are intended to be app
24、lied by surface treatment; Part 1 is required to determine the larvicidial effect of preservatives and Part 2 is required to determine the ovicidal action of the preservatives after egg-laying of young females. Significant technical differences between this document and EN 46-1:2005 are as follows:
25、a) For treatment by dipping sealing of all faces except the one which is exposed to the insects added; b) New Annex E for extending the test duration for slow acting and deferred effect formulations added with the double number of test specimens and half number of insects; c) Requirements for the se
26、lection of test specimens specified with more details in 7.5; d) Preparation of test specimens aligned with EN 46-2 in 8.1; e) Annex D for treating test specimens by pipette deleted. Provisions included in 8.1.3.2. According to the CEN/CENELEC Internal Regulations, the national standards organizatio
27、ns of the following countries are bound to implement this European Standard: Austria, Belgium, Bulgaria, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Latvia, Lithuania, Luxembourg, Malta, Netherlands, Norway, Poland, Portugal, Romania,
28、 Slovakia, Slovenia, Spain, Sweden, Switzerland and the United Kingdom. Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN 46-1:2009EN 46-1:2009 (E) 5 Introduction This Europe
29、an Standard describes a laboratory method of testing which gives a basis for the assessment of the preventive action of a wood preservative, when applied as a surface treatment, against recently hatched larvae of Hylotrupes bajulus, whereas the method for determining the toxic values against Hylotru
30、pes bajulus (EN 47) provides a means of checking whether a preservative prevents attack by these larvae and prevents their survival within totally impregnated wood. This method makes it possible to determine whether recently hatched larvae are capable of boring through the treated surface of a susce
31、ptible wood species and of surviving in the untreated part of the test specimen. For this purpose, the procedure seeks to reproduce normal egg-laying conditions existing in cracks in wood, which provide the principal egg-laying sites. It takes account of the fact that, if larvae pass through the tre
32、ated surface, they will then tunnel in the direction of the least protected regions of the wood. This laboratory method provides one criterion by which the value of a preservative can be assessed. In making this assessment, the methods by which the preservative may be applied should be taken into ac
33、count. This test is of particular interest when applied to test specimens which have been subjected to an ageing procedure. It is further recommended that results from this test should be supplemented by those from other appropriate tests and, above all, by practical experience. When products which
34、are very active at low concentrations are used it is very important to take suitable precautions to isolate and separate, as far as possible, operations involving chemical products, other products, treated wood, laboratory apparatus and clothing. Suitable precautions should include the use of separa
35、te rooms, areas within rooms, extraction facilities, conditioning chambers and special training for personnel (see also Annex D for environmental, health and safety precautions). Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or n
36、etworking permitted without license from IHS-,-,-BS EN 46-1:2009EN 46-1:2009 (E) 6 1 Scope This European Standard specifies a method for the determination of the preventive action of a wood preservative against recently hatched larvae of Hylotrupes bajulus (Linnaeus) when the preservative is applied
37、 as a surface treatment to wood. This method is applicable to: water-insoluble chemicals which are being studied as active insecticides; organic formulations, as supplied or as prepared in the laboratory by dilution of concentrates; organic water-dispersible formulations as supplied or as prepared i
38、n the laboratory by dilution of concentrates; and water-soluble materials, for example salts. The method is applicable whether or not the test specimens have been subjected to appropriate ageing procedures. 2 Normative reference The following referenced documents are indispensable for the applicatio
39、n of this document. For dated references, only the edition cited applies. For undated references, the latest edition of the referenced document (including any amendments) applies. EN 73, Wood preservatives Accelerated ageing tests of treated wood prior to biological testing Evaporative ageing proced
40、ure EN 84, Wood preservatives Accelerated ageing of treated wood prior to biological testing Leaching procedure EN ISO 3696, Water for analytical laboratory use Specification and test methods (ISO 3696:1987) 3 Terms and definitions For the purposes of this document, the following terms and definitio
41、ns apply. 3.1 representative sample sample having its physical and/or chemical characteristics identical to the volumetric average characteristics of the total volume being sampled EN 1001-2:2005, 4.71 3.2 supplier sponsor of the test (person or company providing the sample of wood preservative to b
42、e tested) NOTE Adapted from EN 1001-2:2005, 4.83. Copyright European Committee for Standardization Provided by IHS under license with CENNot for ResaleNo reproduction or networking permitted without license from IHS-,-,-BS EN 46-1:2009EN 46-1:2009 (E) 7 4 Principle Depending on the test being carrie
43、d out either: on a set of test specimens of a susceptible wood species that is surface treated with a solution of the preservative; or if toxic values are to be determined, on several sets of test specimens of a susceptible wood species that are surface treated with a series of solutions in which th
44、e concentration of preservative is ranged in a given progression. The treated test specimens are exposed to recently hatched larvae of Hylotrupes bajulus. The resulting attack is observed and compared with those in untreated control test specimens. If the preservative has been prepared in the labora
45、tory by dilution of a concentrate or by dissolution of a solid, the resulting attack is also compared to that in solvent or diluent treated control test specimens. 5 Test materials 5.1 Biological material 5.1.1 Hylotrupes bajulus (Linnaeus) larvae, within three days of hatching. 5.1.2 Source of larv
46、ae. Obtain the larvae from cultures reared, e.g. as described in Annex B. 5.1.3 Provision of larvae. Collect larvae from eggs laid by different females. 5.1.4 Choice of larvae. Use a mixed batch of these larvae for the test. Use ten larvae per treated test specimen or control test specimen. 5.2 Prod
47、ucts and reagents 5.2.1 Paraffin wax, for fixing the glass plate and for sealing the end faces of test specimens to be treated with solutions in all cases in which water is the continuous phase. NOTE Paraffin wax with a setting point of 52 C to 54 C has been found to be suitable. 5.2.2 Gelatin, for
48、sealing the end faces of test specimens to be treated with solutions in which an organic solvent is the continuous phase. 5.2.3 Water, complying with grade 3 of EN ISO 3696. 5.2.4 Solvent or diluent, a volatile liquid that will dissolve or dilute the preservative but does not leave a residue in the wood at the end of the post-treatment conditioning period that has a toxic effect on the insects. CAUTION Do not use benzen
